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为阐明槐豆木虱(Cyamophila willieti)雌雄成虫基因表达差异及其化学感受机制，本研究通过转录组测序分析雌雄成虫基因表达谱，并聚焦气味结合蛋白(odorant binding proteins,OBPs)基因家族的功能特征。本研究通过转录组测序，构建了槐豆木虱雌雄成虫转录组数据库，对其unigenes的功能注释、分类、差异表达、 GO富集等进行分析，并挖掘槐豆木虱雌雄成虫中潜在的OBPs家族基因，分析其理化性质、保守基序、系统发育及表达量差异。结果显示，雌雄成虫间存在7 230个差异表达基因(differentially expressed genes, DEGs),显著富集于信号转导和脂类代谢通路。共鉴定出22个OBPs基因，其中CwOBP21在雄虫中显著高表达，CwOBP8在雌虫中特异性表达。OBPs蛋白(如CwOBP7和CwOBP12)的理化性质分析表明，其分子量范围为10.2～21.5 kDa,与多种昆虫OBPs一致，符合OBPs的结构特征。系统进化分析显示，CwOBPs与近缘物种存在功能分化，部分基因可能通过水平转移或结构域重组参与嗅觉适应性演化。性别特异性OBPs的表达差异揭示了雌雄成虫在寄主定位、交配行为中的潜在分工，例如雄虫高表达基因可能调控性信息素识别，而雌虫特异基因可能与产卵行为相关。本研究阐明了槐豆木虱OBPs家族的性别偏好性表达模式，为靶向嗅觉干扰的性别特异性防控技术提供了关键分子靶标，对降低化学农药依赖、推动生态防控策略具有重要理论价值。
Abstract：To elucidate the gene expression differences between female and male adults of Cyamophila willieti and their chemical sensing mechanisms, this study utilized transcriptome sequencing for comparative analysis of gene expression profiles in both sexes, with a focus on the functional divergence of the odorant binding proteins(OBPs) gene family. This study constructed a transcriptome database of female and male C. willieti, performed functional annotation, classification, differential expression, and GO enrichment analysis of unigenes, and identified and characterized potential OBPs gene family, analyzed its physicochemical properties, conserved motifs, phylogenetic relationships, and expression level differences. Comparative analysis with the closely related Diaphorina citri revealed distinct features of C. willieti. 7 230 differentially expressed genes(DEGs) between male and female adult insects were identified, significantly enriched in signal transduction and lipid metabolism pathways. A total of 22 OBPs genes were identified. Notably, CwOBP21 was significantly highly expressed in males, while CwOBP8 showed female-specific expression. Analysis of the physicochemical properties of OBPs, such as CwOBP7 and CwOBP12 indicates that their molecular weight ranges from 10.2 to 21.5 kDa, consistent with various insect OBPs and in line with the structural characteristics of OBPs. Phylogenetic analysis indicates that CwOBPs exhibit functional divergence compared to their orthologs in closely related species. Some genes may have contributed to olfactory adaptation through mechanisms such as horizontal gene transfer or domain recombination. Differential expression of sex-specific OBPs suggests a potential beha-vioral differentiation between female and male adults in host location and mating beha-viors. For instance, genes highly expressed in males may regulate pheromone recognition, while female-specific genes are likely asso-ciated with oviposition behavior. This study cla-rifies the unique gender-preferred expression patterns of the OBPs family in C. willieti and provides key molecular targets for developing sex-specific control strategies based on olfactory interference. These findings hold significant theoretical value for reducing dependence on chemical pesticides and promoting ecological pest management.
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基本信息:
DOI：10.13417/j.gab.044.000908
中图分类号:S433.3
引用信息:
[1]杨清川,叶婷玉,古力努尔·吐尔洪,等.槐豆木虱雌雄成虫转录组及气味结合蛋白基因家族分析[J].基因组学与应用生物学,2025,44(09):908-922.DOI:10.13417/j.gab.044.000908.
基金信息:
兵团财政科技计划项目南疆农业有害生物综合治理兵团重点实验室创新能力提升建设项目(2023ZYYD-3)资助