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为明确深圳某海马养殖基地线纹海马(Hippocampus erectus)突发性死亡病因,本研究从患病死亡线纹海马病灶组织分离到一株优势菌SZVA20190621,随后对分离菌的形态特征、生理生化特性、回归感染、16S r RNA基因进化及药物敏感性进行研究。结果显示,该菌葡萄糖产气、甘露醇、覃糖、蔗糖、氧化酶活性和MR等生理生化鉴定为阳性;与NCBI上溶藻弧菌(Vibrio alginolyticus) KT986151具有98.16%的同源性;回归感染实验表明菌株SZVA20190621对线纹海马具有强致病性,LD50为3.73×105CFU/mL。综上结果,鉴定该菌为致病性溶藻弧菌。药敏结果显示,该菌株对头孢唑林、头孢噻吩、哌拉西林、大观霉素、阿米卡星、克拉霉素、克林霉素及呋喃妥因耐药;对氨曲南、头孢西丁、头孢呋辛、头孢曲松、卡那霉素、红霉素中度敏感;对头孢他啶、头孢噻肟、头孢哌酮、头孢吡肟、链霉素、妥布霉素、庆大霉素、复方新诺明、氯霉素等17种抗生素高度敏感。本研究为线纹海马养殖中溶藻弧菌病的防控提供一定的参考依据。
Abstract：In order to det ermi ne the cause of Hippocampus erectus suddenly death, we isolated a dominant bacterium SZVA20190621 from the diseased part of it, and then studied the morphological characteristics, physiological and biochemical characteristics, regression infection, 16 S rRNA gene evolution and drug sensitivity. The results showed that the strain produced glucose, mannitol, Trehalose anhydrous sucrose, oxidase activity and MR test were positive for physiological and biochemical identification; had 98.16% homology with Vibrio alginolyticus KT986151 on NCBI. Regression infection test showed that the strain SZVA20190621 was pathogenic to Hippocampus erectus, and its LD50 was 3.73×105 CFU/mL. In summary, the strain was identified as a pathogenic Vibrio alginolyticus. The results of drug susceptibility test showed that the strain was resistant to cefazolin,cefalotin, piperacillin, spectinomycin, amikacin, clarithromycin, clindamycin and furadantin; moderately sensitive to aztreonam, cefoxitin, cefatriaxone, kanamycin and erythromycin; highly sensitive to 17 different antibiotics such as ceftazidime, cefotaxime, cefoperazone, cefepime, streptomycin, tobramycin, gentamycin, bactrim and chloramghenicol. This study will provide some reference basis for the prevention and control of disease induced by Vibrio alginolyticus in Hippocampus erectus culture.
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基本信息:
DOI：10.13417/j.gab.040.001679
中图分类号:S858.94
引用信息:
[1]雷美华,喻大鹏,邓国华,等.线纹海马源溶藻弧菌的分离、鉴定及药敏分析[J].基因组学与应用生物学,2021,40(04):1679-1686.DOI:10.13417/j.gab.040.001679.
基金信息:
深圳市事业单位年度专项预算资金项目(008030); 深圳市科技计划项目(JCYJ20180306173022502); 国家自然科学基金项目(U1401211)共同资助
2019-12-23
2019-12-23
2019-12-23