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日本青鳉(Oryzias latipes)性腺体细胞衍生因子(gonadal soma-derived factor,gsdf)缺失导致生殖细胞囊性分裂过度增殖和精母细胞中心粒复制异常。为探究gsdf信号如何影响细胞增殖和中心粒复制，本研究利用实时荧光定量PCR(real time fluorogenic quantitative PCR)和免疫荧光检测等方法，分析了极光激酶A(aurora kinase A,aurka)在野生型(wild-type, WT)和gsdf缺失型日本青鳉性腺中的差异表达。实时荧光定量PCR显示日本青鳉aurka基因虽然在多个组织中均有表达，但主要集中在精巢表达，且与野生型相比，在gsdf缺失型精巢中的表达量显著升高。免疫荧光检测显示日本青鳉抗Aurka信号在卵巢中定位于减数分裂前期双线期卵母细胞细胞核，在精巢中定位于精原细胞和精母细胞核周质表达。与野生型相比，gsdf缺失型XY巨大精巢的免疫反应信号上升，且gsdf缺失型XY巨大精巢中有大量A型精母细胞(type A spermatocytes, SpcA)染色体联会异常。综上，日本青鳉gsdf信号对雄性减数分裂前期生殖细胞具有负调控机制，Gsdf(TGF-β)-Aurka信号参与调控雄性生殖细胞增殖和G2/M期检查点滞留。本研究为雄性配子发生发育的分子调控机制提供了新的线索。
Abstract：Loss of gonadal soma-derived factor(gsdf) leads to extensive cystic division and abnormality of centriole replication in germ cells of Japanese medaka(Oryzias latipes).To investigate the role of gsdf involved in germ cell division and centriole replication, the differential expression of aurora kinase A(aurka) in the gonads of wild-type(WT) and gsdf-deficient Japanese medaka was analyzed by real time fluorogenic quantitative PCR and immunofluorescence detection. The real time fluorogenic quantitative PCR results showed that although the aurka gene was expressed in multiple tissues of Japanese medaka, it was mainly concetrated in the testis, and the expression level was significantly increased in gsdf deficient testes than that in wild-type testes. Immunofluorescence analysis showed that the anti-Aurka signals of Japanese medaka were mainly localized in the nuclei of female oocytes during the premeiotic diplotene stage in the ovary, and localized in the cytoplasm of spermatogonia and spermatocytes in the testis. Compared to the wild type, the immune response signal of gsdf-deficient XY giant testes increased, and there were a large number of type A spermatocytes(SpcA) with abnormal chromosome synapses in gsdf-deficient testes. In summary, the gsdf signal in Japanese medaka negatively regulates the premeiotic germ cells in males, and the Gsdf(TGF-β)-Aurka signaling pathway is involved in regulating male germ cell proliferation and G2/M checkpoint retention.This study provides new insights into the molecular regulatory mechanisms of male gametogenesis and development.
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基本信息:
DOI：10.13417/j.gab.044.000878
中图分类号:S917.4
引用信息:
[1]陈娟,李晨杰,余世琦,等.日本青鳉Gsdf-Aurka信号调控减数分裂前期生殖细胞雌雄分化的潜在分子机制[J].基因组学与应用生物学,2025,44(09):878-888.DOI:10.13417/j.gab.044.000878.
基金信息:
国家重点研发计划项目(2018YFD0900601)资助