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虫草蝠蛾（Hepialus armoricanus）是冬虫夏草（Ophiocordyceps sinensis）的寄主昆虫，光照作为关键环境因子对其虫卵孵化的调控机制尚不明确。以虫草蝠蛾虫卵、黑暗孵化组和光照孵化组的幼虫为材料，利用Illumina HiSeq平台进行转录组测序。通过组装和注释筛选差异表达基因（differentially expressed genes， DEGs），并利用GO、KEGG数据库进行功能富集分析，重点关注与光照响应相关的基因和通路。差异表达分析显示，光照显著影响关键基因表达：与黑暗条件相比，光照组孵化的幼虫其细胞色素b5（cytochrome b5）和细胞色素c（cytochrome c）表达上调，而细胞色素P450（cytochrome P450）表达下调；几丁质合成通路相关基因，如几丁质酶基因、海藻糖酶基因、几丁质合成酶基因多上调表达；与昆虫发育密切相关的保幼激素和蜕皮激素通路基因也呈现差异表达。这些变化表明光照主要通过调节虫卵内能量代谢（细胞色素通路）、表皮结构形成（几丁质代谢）和发育激素信号等生物学过程影响孵化进程。本研究揭示了光照通过差异调控细胞色素家族、几丁质代谢及昆虫激素相关基因表达，进而影响虫草蝠蛾虫卵孵化的分子机制，为理解光照调控昆虫发育提供了分子基础。
Abstract：Hepialus armoricanus is the host insect of Ophiocordyceps sinensis， yet the regulatory mechanism of light， a key environmental factor， on its egg hatching remains unclear. In this study， transcriptome sequencing was performed on the Illumina HiSeq platform using eggs of Hepialus armoricanus and larvae from dark-hatched and light-hatched groups as experimental materials. Differentially expressed genes (DEGs) were screened through assembly and annotation， followed by functional enrichment analysis using GO and KEGG databases， with a focus on genes and pathways associated with light responses. Differential expression analysis revealed that illumination significantly affected key gene expression: compared with dark conditions， larvae hatched under light conditions showed upregulated expression of cytochrome b5 and cytochrome c， whereas cytochrome P450 expression was downregulated; genes related to the chitin biosynthesis pathway， such as chitinase genes， trehalase genes， and chitin synthase genes were predominantly upregulated; genes involved in the juvenile hormone and ecdysone signaling pathways， which are closely related to insect development， also exhibited differential expression. These findings suggest that light primarily influences the hatching process by modulating energy metabolism (cytochrome pathways)， cuticle formation (chitin metabolism)， and developmental hormone signaling during embryonic development. This study reveals the molecular mechanisms by which light affects Hepialus armoricanus egg hatching through differential regulation of the cytochrome gene family， chitin metabolism， and insect hormone-related gene expression， providing a molecular basis for understanding light-mediated regulation of insect development.
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基本信息:
中图分类号:S567.35
引用信息:
[1]陈鑫鹏,徐菲菲,高继海,等.基于转录组的光照调控虫草蝠蛾虫卵孵化相关差异表达基因分析[J].基因组学与应用生物学().
基金信息:
四川省科技厅基础研究项目（2023JDR0053）资助
2026-07-28
2026-07-28
2026-07-28