| 204 | 5 | 29 |
| 下载次数 | 被引频次 | 阅读次数 |
本研究利用基因挖掘技术从球孢白僵菌(Beauveria bassiana)基因组中获得聚酮合酶(PKS)基因,并对这些基因序列进行生物信息学分析预测其功能,同时检测这些基因在不同培养基培养条件下的表达情况。结果显示:球孢白僵菌中含有13个PKS (Bdass1～13)基因,结构域分析显示球孢白僵菌中有还原型PKS 8个,非还原型PKS 2个,杂合型NRPS/PKS 3个;聚类分析显示Bdass8参与卵孢白僵菌素生物合成,Bdass5可能参与洛伐他汀九酮体的生物合成、Bdass4可能参与伏马菌素的生物合成、Bdass11可能参与phenolthiocerol的生物合成;非还原型PKS中Bdass7和Bdass10可能参与分生孢子色素的合成,Bdass1、Bdass2、Bdass3、Bdass6、Bdass9、Bdass12、Bdass13分别与其他未知聚酮合酶形成独立的分支;不同的PKS基因在不同培养基培养条件下其表达情况差异非常显著,如Bdass8、Bdass10和Bdass11在5种培养基上均强烈表达,Bdass4、Bdass6在5种培养基上微弱表达,Bdass1、Bdass5、Bdass11、Bdass12、Bdass13仅在几种培养基上表达,Bdass2仅在INO培养上微弱表达,Bdass3、Bdass7在5种培养基上均不表达。该研究为球孢白僵菌中PKS基因的功能鉴定奠定基础。
Abstract：The present text has got polyketides synthase(PKS) genes from the genome of Beauveria bassiana with the method of genome-mining and analyzed these genes to predict their function by the bioinformatic analysis,simultaneously tested the condition of these gene expression in the different medium. The results showed that there were 13 PKS gene in the genome of B. bassiana, including 8 reducing PKS(R-PKS), 2 non-reducing PKS(NR-PKS) and 3 hybrid NRPS/PKS; the phylogenetic tree revealed that Bdass8 involved in the biosynthesis of tenellin, Bdass5 might involve the biosynthesis of lovastatin ninoketone, Bdass4 could involve the biosynthesis of fumonisin, and Bdass11 might involve the biosynthesis of phenolthiocerol among the reducing PKS; and among the nonreducing PKS Bdass7 and Bdass10 could involve the biosynthesis of conidial yellow pigment; additionally,Bdass1, Bdass2, Bdass3, Bdass6, Bdass9, Bdass12, Bdass13 clustered with some unkown product that they involved together, respectively; the gene expression had significant difference when the different PKS genes were cultivating in the different medium, for example, Bdass8, Bdass10 and Bdass11 was strongly expressed cultivating in the 5 medium, Bdass4, Bdass6 was slightly expressed in all medium, Bdass1, Bdass5, Bdass11, Bdass12 and Bdass13 was expressed in some special medim, Bdass2 was only expressed in the INO, Bdass3 and Bdass7 were never expressed in all medium. The study took some basis for the PKS gene function of B. bassiana.
Adane K.,Moore D.,and Archer S.A.,1996,Preliminary studieson the use of Beauveria bassiana to control Sitophilus ze amais(Coleoptera:Curculionidae)in the laboratory,J.Stored Prod.Res.,32(2):105-113
Amnuaykanjanasin A.,Phonghanpot S.,Sengpanich N.,Cheevadhanarak S.,and Tanticharoen M.,2009,Insect-specific polyketide synthases(PKSs),potential PKS-nonribosomal peptide synthetase hybrids,and novel PKS clades in tropical fungi,Appl.Environ.Microbiol.,75(11):3721-3732
Blin K.,Medema M.H.,Kazempour D.,Fischbach M.A.,Breitling R.,Takano E.,and Weber T.,2013,antiSMASH 2.0-a versatile platform for genome mining of secondary metabolite producers,Nucleic Acids Res.,41:W204-W214
Campos R.A.,Arruda W.,Boldo J.T.,da Silva M.V.,de Barros N.M.,de Azevedo J.L.,Schrank A.,and Vainstein M.H.,2005,Boophilus microplus infection by Beauveria amorpha and Beauveria bassiana:SEM analysis and regulation of subtilisin-like proteases and chitinases,Curr.Microbiol.,50(5):257-261
Charnley A.K.,2003,Fungal pathogens of insects:Cuticle degrading enzymes and toxins,Advances in Botanical Research,40(5):241-321
Cox R.J.,2007,Polyketides,proteins and genes in fungi:Programmed nano-machines begin to reveal their secrets,Org.Biomol.Chem.,5(13):2010-2026
Eley K.L.,Halo L.M.,Song Z.,Powles H.,Cox R.J.,Bailey A.M.,Lazarus C.M.,and Simpson T.J.,2007,Biosynthesis of the2-pyridone tenellin in the insect pathogenic fungus Be auveria bassiana,Chem.Bio.Chem.,8(3):289-297
Gillespie J.P.,Bailey A.M.,Cobb B.,and Vilcinskas A.,2000,Fungi as elicitors of insect immune responses,Arch.Insect Biochem.Physiol.,44(2):49-68
Kinoshita Y.,Yamamoto Y.,Kurokawa T.,and Yoshimura I.,2001,Influence of nitrogen sources on usnic acid production in a cultured mycobiont of the lichen Usnea hirta(L.)Wigg,Biosci.Biotechnol.Biochem.,65(8):1900-1902
Kirimura K.,Watanabe S.,and Kobayashi K.,2016,Heterologous gene expression and functional analysis of a typeⅢpolyketide synthase from Aspergillus niger NRRL 328,Biochem.Biophys.Res.Commun.,473(4):1 106-11 10
Kroken S.,Glass N.L.,Taylor J.W.,Yoder O.C.,and Turgeon B.G.,2003,Phylogenomic analysis of typeⅠpolyketide synthase genes in pathogenic and saprobic ascomycetes,Proc.Nati.Acad.Sci.USA,100(26):15670-15675
Liu D.D.,Duan Y.X.,and Chen L.J.,2007,Determine nematicidal of ferment liquid of Snf907-76 and isolation and purification nematocide,Nongyao(Agrochemicals),46(9):644-646(刘丹丹,段玉玺,陈立杰,2007,Snf 907-76发酵液杀线虫活性测定及活性物质分离纯化,农药,46(9):644-646)
Liu W.C.,Yang F.,Zhang R.,Shi X.,Lu X.H.,Luan Y.S.,Xiu Z.L.,and Dong Y.S.,2016,Production of polyketides with anthelmintic activity by the fungus Talaromyces wortmannii using one strain-many compounds(OSMAC)method,Phytochem.Lett.,18:157-161
Marnix H.M.,Blin K.,Cimermancic P.,de Jager V.,Zakrzewski P.,Fischbach M.A.,Weber T.,Takano E.,and Breitling R.,2011,antiSMASH:Rapid identification,annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences,Nucleic.Acids.Res.,39(2):W339-W346
Rohlfs M.,and Churchill A.C.,2011,Fungal secondary metabolites as modulators of interactions with insects and other arthropods,Fungal Genet.Biol.,48(1):23-34
Sato M.,Dander J.E.,Sato C.,Hung Y.S.,Gao S.S.,Tang M.C.,Hang L.,Winter J.M.,Garg N.K.,Watanabe K.,and Tang Y.,2017,Collaborative biosynthesis of maleimide-and succinimide-containing natural products by fungal polyketide megasynthases,J.Am.Chem.Soc.,139(15):53 17-5320
Song X.B.,Peng A.T.,Cheng B.P.,Ling J.F.,Chen X.,and Zhang L.H.,2017,Isolation and identification of a Be auveria bassiana strain infecting Diaphorina citri,Zhiwu Baohu(Plant Protection),43(4):139-144(宋晓兵,彭埃天,程保平,凌金锋,陈霞,张炼辉,2017,一株侵染柑橘木虱的球孢白僵菌的分离及鉴定,植物保护,43(4):139-144)
Wang G.Q.,Zhou E.H.,Luo W.G.,and Liu X.S.,2015,Efficacy trial of Beauveria bassiana against whitefly in greenhouse,Shengwu Zhaihai Kexue(Biological Disaster Science),38(3):217-220(王国庆,周尔槐,罗稳根,刘新生,2015,白僵菌防治温室大棚白粉虱药效试验,生物灾害科学,38(3):217-220)
Wang Y.,Wen B.,Ma J.,Zhang Y.H.,and Wu Y.F.,2017,Con trol effects of Be auveria bassiana on 4 conservatory pests in frigid regions,Shengwu Zhaihai Kexue(Biological Disaster Science),40(4):284-287(王月,文斌,马军,张跃华,吴云飞,2017,球孢白僵菌(Beauveria bassian)对寒地温室4种害虫防控研究,生物灾害科学,40(4):284-287)
Wang Y.,Wang J.,Cheong Y.H.,and Hur J.S.,2014,Three new non-reducing polyketide synthase genes from the lichenforming fungus Usnea longissima,Mycobiology,42(1):34-40
Weber T.,Blin K.,Duddela S.,Krug D.,Kim U.H.,Bruccoleri R.,Lee S.Y.,Fischbach M.A.,Muller R.,Wohlleben W.,Brertling R.,Takano E.,and Medema M.H.,2015,antiSMASH 3.0-a comprehensive resource for the genome mining of biosynthetic gene clusters,Nucleic Acids Res.,43(W1):W237-W243
Xiao G.,Ying S.H.,Zheng P.,Wang Z.L.,Zhang S.,Zhang S.W.,Xie X.Q.,Shang Y.,Leger R.J.,Zhao G.P.,Wang C.,and Feng M.G.,2012,Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana,Sci.Rep.,2:483
Yuan C.,Guo Y.H.,Wang H.Y.,Ma X.J.,Jiang T.,Zhao J.L.,Zou Z.M.,and Ding G.,2016,Allelopathic polyketides from an endolichenic fungus Myxotrichum sp.by using OSMAC strategy,Sci.Rep.,6:19350
Yuan X.L.,Zhao N.,Chen J.,Wang J.,Yang Y.M.,and Wang Y.,2017,Bioinformatics analysis of NRPS gene diversity in Trichoderma harzianum,Xibu Linye Kexue(Journal of West China Forestry Science),(4):7-12,17(原晓龙,赵能,陈剑,王娟,杨宇明,王毅,2017,哈茨木霉基因组中基因多样性的生物信息学分析,西部林业科学,(4):7-12,17)
Zuloaga F.O.,Salariato D.L.,and Scataglini A.,2018,Molecular phylogeny of Panicum sensu stricto(Poaceae,Panicoideae,Paniceae)and insights into its biogeography and evolution,PLoS One,13(2):e0191529
基本信息:
DOI：10.13417/j.gab.039.000606
中图分类号:S476.12
引用信息:
[1]原晓龙,李云琴,王毅.球孢白僵菌中聚酮合酶基因多样性分析[J].基因组学与应用生物学,2020,39(02):606-613.DOI:10.13417/j.gab.039.000606.
基金信息:
国家自然科学基金项目(31860177);; 云南省应用基础研究计划面上项目(2016FB055)共同资助
2020-02-25
2020-02-25