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采用水提醇沉法得到党参粗多糖(COP),采用Sevag法除去蛋白成份,接着通过Sephacry1 S-200HR及Sephadex G-25凝胶柱色谱分离得到均一多糖COP-1。凝胶渗透色谱法(HPGPC)测定其纯度和平均分子量。高效液相色谱法(HPLC)确定其单糖的组成。红外光谱及核磁推测COP-1结构。结果表明COP-1平均分子量约为2.1×103 Da,且均由阶D-(2→1)呋喃果糖组成。
Abstract：Crude Codonopsis pilosula polysaccharide was gained using water extraction-alcohol precipitation,and then Sevag method was used to remove protein.The main fraction of Codonopsis pilosula polysaccharide,namely COP-1,was isolated by Sephacryl S- 200 HR and Sephadex G- 25 column chromatography.High Performance Gel Permeation Chromatography(HPGPC) was used to determinate the purity and molecular weight of COP-1.High Performance Liquid Chromatography(HPLC) was used to analyze the monosaccharide composition of COP-1.Its structure was elucidated by FT-IR and NMR(1H-NMR,13C-NMR,HMBC and HSQC).The molecular weight of COP-1 was about 2.1×103 Da,and the mainly constitution of it was β-D-(2→1) fructose.
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基本信息:
DOI：10.13417/j.gab.035.001294
中图分类号:R284
引用信息:
[1]刘章泉,姚晓东,肖世基,等.一种水溶性党参多糖的分离纯化及结构分析[J].基因组学与应用生物学,2016,35(06):1294-1299.DOI:10.13417/j.gab.035.001294.
基金信息:
遵义医学院博士启动资金协助项目(ZMKD2013-006);; 贵州省自然科学基金(QKH-LH[2014]7563)共同资助
2016-06-25
2016-06-25