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低温胁迫是制约水稻产量和地理分布的重要非生物因素。bHLH转录因子家族在植物逆境响应中发挥关键作用。本团队前期研究发现，自然变异的OsbHLH150通过调控ABA合成基因OsNCED3的表达正调控水稻耐冷性。在此基础上，本研究发现OsbHLH150还直接结合ABA降解基因OsABA8ox2启动子上的E-box元件并激活其表达，表明该转录因子同时参与低温下ABA的合成与降解代谢。进一步分析显示，不同OsbHLH150单倍型中OsNCED3的转录水平与蛋白丰度不完全一致，提示转录后调控参与耐冷性差异的形成。此外，ABA含量与耐冷存活率并非完全正相关，表明ABA是耐冷性的必要条件但非唯一决定因素。本研究揭示了OsbHLH150介导的ABA代谢调控网络的复杂性，为水稻耐冷分子育种提供了重要基因资源。
Abstract：Cold stress is a major abiotic factor limiting rice yield and geographical distribution. The bHLH transcription factor family plays critical roles in plant stress responses. Our previous study revealed that naturally varied OsbHLH150 positively regulates cold tolerance by modulating the expression of the ABA biosynthesis gene OsNCED3. In this study， we further demonstrate that OsbHLH150 directly binds to the E-box element in the promoter of the ABA catabolism gene OsABA8ox2 and activates its expression， indicating that OsbHLH150 participates in both ABA biosynthesis and catabolism under cold stress. Moreover， the inconsistency between OsNCED3 transcript levels and protein abundance across different haplotypes suggests the involvement of post-transcriptional regulation in cold tolerance divergence. Additionally， the lack of a perfect correlation between ABA content and survival rate implies that ABA accumulation is necessary but not sufficient for cold tolerance. Collectively， this study uncovers the complexity of OsbHLH150-mediated ABA metabolic regulation and provides valuable genetic resources for cold-tolerant rice breeding.
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基本信息:
中图分类号:S511;Q943.2
引用信息:
[1]孙津梁,周子琦,李明裕,等.一个自然变异的基因OsbHLH150介导调控冷胁迫下ABA的合成和降解代谢提高水稻耐冷性[J].基因组学与应用生物学().
基金信息:
广西中央引导地方专项（桂科-ZY23055027）; 广西科技计划（桂科-AD25069107）; 广西自然科学基金（2024GXNSFAA010473）共同资助
2026-08-26
2026-08-26
2026-08-26