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利用转录组筛选宁夏地方品种静原鸡肉品风味的相关差异基因,了解差异基因对肉品风味转录调控的影响。采集15只静原鸡胸肌和腿肌,利用RNA-seq技术,通过GO、KEGG富集和蛋白网络互作等生物信息学分析筛选调控肉品风味相关的差异表达基因。结果表明:静原鸡胸肌、腿肌中显著差异表达基因总数为2 098个,其中,与腿肌相比,胸肌有1 146个基因显著上调(P<0.05),952个基因显著下调(P<0.05)。GO功能注释中磷代谢占生物学过程最多,肌动蛋白细胞骨架和肌动蛋白肌球蛋白复合体极显著富集在细胞组分中(P<0.01),激酶活性占分子功能最多。KEGG功能注释发现差异基因显著富集到糖酵解和糖异生途径、心肌收缩、紧密接头、甘油磷脂代谢途径等通路中,最终筛选出PGM1、PFKM、PFKL、GPI和ETNPPL等5个可能与静原鸡肉品风味相关的显著上调基因。以上研究结果为阐明其与肉品风味的关系及进一步研究静原鸡肉品风味相关调控基因提供理论依据。
Abstract：The transcriptome was used to screen the differential gene related to the flavor of Ningxia local variety Jingyuan chicken meat, and to understand the influence of differential genes on the transcriptional regulation of meat flavor. 15 chest muscles and leg muscles of Jingyuan chicken were collected, and differentially expressed genes related to meat flavor were screened by bioinformatics analysis such as GO, KEGG enrichment and protein network interaction using RNA-seq technology. The results showed that the total number of differentially expressed genes in the chest muscles and leg muscles of Jingyuan chicken was 2 098. Compared with leg muscle, 1 146 genes were significantly up-regulated(P<0.05) and 952 genes were significantly down-regulated(P<0.05) in chest muscles. Phosphorus metabolism in the GO function annotation accounted for the most biological processes. The actin cytoskeleton and actin myosin complex were highly enriched in the cellular components(P<0.01), and the kinase activity accounted for the most molecular function. The KEGG function annotation revealed that the differential genes were significantly enriched in the pathways of glycolysis and gluconeogenesis, myocardial contraction, tight junctions, and glycerophospholipid metabolic pathways, and finally selected 5 possible significant up-regulation of genes related to chicken flavour such as PGM1, PFKM, PFKL, GPI, and ETNPPL. The above results provide a theoretical basis for elucidating its relationship with meat flavor and further research on the regulation of genes related to flavor of chicken meat.
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基本信息:
DOI：10.13417/j.gab.040.002038
中图分类号:S831.2
引用信息:
[1]虎红红,母童,马正旭,等.基于RNA-seq技术对静原鸡不同部位肉质相关差异基因的筛选[J].基因组学与应用生物学,2021,40(Z1):2038-2046.DOI:10.13417/j.gab.040.002038.
基金信息:
本研究由宁夏自然科学基金项目(2019AAC03070)资助
2019-11-07
2019-11-07
2019-11-07