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双组分信号转导系统(two-component system, TCS)是细菌感知和响应环境的普遍机制,由组氨酸激酶(histidine kinase, HK)和应答调控蛋白(response regulator, RR)组成,它们能够使机体根据环境条件的改变做出适当的细胞反应。嗜根考克氏菌(Kocuria rhizophila)是一种抗生素活性测试的常用质量控制菌株,但目前有关它的TCS研究尚不多见。为研究TCSs在细菌生存适应中的潜在作用,采用生物信息学的方法对已完成基因组测序的8个嗜根考克氏菌菌株的TCSs进行数量统计、结构分析以及功能预测。研究结果显示,嗜根考克氏菌中共存在13种TCSs,且HKs和RRs都是模块化蛋白质,参与外界压力应答、细胞壁合成、酶活性、物质代谢与运输等生命活动。本研究关于TCSs的研究将有助于系统的理解嗜根考克氏菌以及相关物种,为构建该菌的TCS调控网络提供依据,并可推动其在工业上的应用。
Abstract：The two-component system(TCS) is a universal mechanism for bacterial sensing that enable the organism to respond appropriately to changes in different conditions. It consists of histidine kinase(HK) and response regulator(RR). Kocuria rhizophila is a quality control strain commonly used for antibiotic activity testing,but there is little known on TCS. Due to clarify their potential roles in the bacterial adaptation for the survival, the TCSs of eight strains of Kocuria rhizophila which have been sequenced were analyzed quantitatively, structurally and functionally by bioinformatics. The results showed that there were 13 kinds of TCSs in Kocuria rhizophila,which participated in external pressure response, cell wall synthesis, enzyme activity, material metabolism and transportation. What's more, HKs and RRs were modular proteins. Detailed knowledge on two-component signal transduction therefore will contribute to the systemic understanding of Kocuria rhizophila and related species, a basis for the construction of TCS regulatory network and the promotion of its application in industrial.
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基本信息:
DOI：10.13417/j.gab.039.003524
中图分类号:Q933
引用信息:
[1]陈宝花,邹婷婷,黄运红,等.嗜根考克氏菌双组分信号转导系统的生物信息学分析[J].基因组学与应用生物学,2020,39(08):3524-3531.DOI:10.13417/j.gab.039.003524.
基金信息:
国家自然科学基金项目(31360018);; 江西省重点研发项目(20161BBF60076);; 江西师范大学研究生创新基金(YJS2018081)共同资助
2020-08-25
2020-08-25