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植物内源激素是植物体内合成的调节植物生长发育及生物、非生物因素应答的一系列痕量有机化合物,因此,对植物激素的准确定量尤为重要。在这篇文章中,我们一方面就多年来的植物激素检测方法作了简单总结与概述,并对存在的问题及今后的发展方向提出了个人见解,以期为植物激素检测研究提供参考依据;另一方面,液相色谱与质谱的联用是当今世界激素检测应用最广泛、认可度最高的方法,该方法具有较高的灵敏度与选择性,省时省力,我们预测用液相色谱串联质谱法同时检测多种植物激素将是未来发展的趋势。
Abstract：The ability to measure plant hormones quantitatively is crucial as plant endogenous hormones, a series of trace organic compounds synthesized in plants, regulate plant growth, development and response to biotic and abiotic cues. In this paper, on the one hand, we reviewed and summarized the detection methods over the years and discussed the challenges and prospects in order to providing a reference index for the future study. The liquid chromatography with mass spectrometry(LC-MS) is widely used to the detection of plant hormones in today's world. This method has high sensitivity and high selectivity, and can save time and workload. So as a result, we argue that it will be a tendency of the future to detect a variety of plant hormones at the same time by using liquid chromatography with mass spectrometry(LC-MS).
Badenoch-Jones J.,Parker C.W.,and Letham D.S.,1987,Use of isopentenyladenosine and dihydrozeatin riboside antibodies for the quantification of cytokinins,Journal of Plant Growth Regulation,6(3):159-182
Bai Y.,Du F.Y.,and Liu H.W.,2010,Recent development in determination of plant hormones,Shengming Kexue(Chinese Bulletin of Life Sciences),22(1):36-44(白玉,杜甫佑,刘虎威,2010,植物激素检测技术研究进展,生命科学,22(1):36-44)
Boerjan W.,Genetello C.,Van Montagu M.,and Inze D.,1992,A new bioassay for auxins and cytokinins,Plant Physiology,99(3):1090-1098
Brenner M.L.,1981,Modern methods for plant growth substance analysis,Annual Review of Plant Physiology,32:511-538
Cen W.J.,Fan X.S.,Zhu P.C.,Qin S.,and Wang J.,2013,Simultaneous determination of five effective components in Yiqiyangxue oral liquid by ultra performance liquid chromatography with tandem mass spectrometry,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),32(4):557-561(岑卫健,范晓苏,朱平川,覃莎,王锦,2013,UPLC-MS/MS法同时测定益气养血口服液中的5种有效成分,基因组学与应用生物学,32(4):557-561)
Chen K.S.,Xu C.J.,Li F.,Chen Q.J.,and Zhang S.L.,2003,An improved HPLC method for analyzing endogenous IAA and ABA in fruit tissues,Guoshu Xuebao(Journal of Fruit Science),20(1):4-7(陈昆松,徐昌杰,李方,陈青俊,张上隆,2003,HPLC法检测果实组织中内源IAA,ABA方法的改进,果树学报,20(1):4-7)
Chen X.M.,and Wang S.S.,1992,Quantitative analysis of ABA,IAA and NAA in plant tissues by HPLC,Zhiwu Shengli Xuebao(Plant Physiol.J.),28(5):368-371(陈雪梅,王沙生,1992,HPLC法定量分析植物组织中ABA,IAA和NAA,植物生理学报,28(5):368-371)
Chen Y.F.,and Zhou X.,1995,Quantitative measurement for cytokinins using TLC and ELISA,Zhiwu Shengli Xuebao(Plant Physiol.J.),31(4):289-291(陈以峰,周燮,1995,薄板层析和酶联免疫吸附法连用测定细胞分裂素,植物生理学报,31(4):289-291)
Chiwocha S.D.,Abrams S.R.,Ambrose S.J.,Cutler A.J.,Loewen M.,Ross A.R.,and Kermode A.R.,2003,A method for profiling classes of plant hormones and their metabolites using liquid chromatography-electrospray ionization tandem mass spectrometry:an analysis of hormone regulation of thermodormancy of lettuce(Lactuca sativa L.)seeds,Plant J.,35(3):405-417
Daie J.,and Wyse R.,1982,Adaptation of the enzyme-linked immunosorbent assay(ELISA)to the quantitative analysis of abscisic acid,Analytical Biochemistry,119(2):365-371
Davis G.C.,Hein M.B.,and Chapman D.A.,1986,Evaluation of immunosorbents for the analysis of small molecules:isolation and purification of cytokinins,J.Chromatogr.,366:171-189
Deng W.H.,and Zhang J.Q.,2015,Determination of four endogenous phytohormones in Artemisia ordosica leaves by ultra-performance liquid chromatography-tandem mass spectrometry,Zhiwuxue Yanjiu(Botanical Research),4:1-7(邓文红,张俊琦,2015,UPLC-MS/MS测定油篙叶片中4种内源植物激素,植物学研究,4:1-7)
Du L.M.,and Xu Q.Q.,2000,Separation and determination of three plant internal hormones by gas chromatography,Sepu(Chinese Journal of Chromatography),18(1):67-69(杜黎明,许庆琴,2000,气相色谱法分离和测定3种植物内源激素,色谱,18(1):67-69)
Durgbanshi A.,Arbona V.,Pozo O.,Miersch O.,Sancho J.V.,and Gomez-Cadenas A.,2005,Simultaneous determination of multiple phytohormones in plant extracts by liquid chromatography-electrospray tandem mass spectrometry,J.Agric.Food Chem.,53(22):8437-8442
Ge L.,Peh C.Y.,Yong J.W.,Tan S.N.,Hua L.,and Ong E.S.,2007,Analyses of gibberellins by capillary electrophoresis-mass spectrometry combined with solid-phase extraction,J.Chromatogr.A,1159(1):242-249
Gupta V.,Kumar M.,Brahmbhatt H.,Reddy C.R.K.,Seth A.,and Jha B.,2011,Simultaneous determination of different endogenetic plant growth regulators in common green seaweeds using dispersive liquid-liquid microextraction method,Plant Physiol.Biochem.,49(11):1259-1263
Hou S.,Zhu J.,Ding M.,and Lv G.,2008,Simultaneous determination of gibberellic acid,indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography-electrospray tandem mass spectrometry,Talanta,76(4):798-802
Hoyerova K.,Gaudinova A.,Malbeck J.,Dobrev P.I.,Kocabek T.,Solcova B.,and Kaminek M.,2006,Efficiency of different methods of extraction and purification of cytokinins,Phytochemistry,67(11):1151-1159
Hradecka V.,Novak O.,Havlicek L.,and Strnad M.,2007,Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography-mass spectrometry,J.Chromatogr.B,847(2):162-173
Huang D.P.,Xu H.,and Jiang Y.F.,2011,Determination of four endogenous hormones in honey floral bud of Guangxi by UPLC,Dazhong Keji(Popular Science&Technology),(10):184-185(黄岛平,徐慧,蒋艳芳,2011,超高效液相色谱法测定广西蜜梨花芽中4种植物激素,大众科技,(10):184-185)
Kampbell D.H.,and Vandegrift S.A.,1998,Analysis of dissolved methane,ethane,and ethylene in ground water by a standard gas chromatographic technique,J.Chromatogr.Science,36(5):253-256
Kohn J.,and Wilchek M.,1982,A new approach(cyano-transfer)for cyanogen bromide activation of Sepharose at neutral pH,which yields activated resins,free of interfering nitrogen derivatives,Biochem.Biophys.Res.Commun.,107(3):878-884
Kojima M.,Kamada-Nobusada T.,Komatsu H.,Takei K.,Kuroha T.,Mizutani M.,and Sakakibara H.,2009,Highly sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry:an application for hormone profiling in Oryza saliva,Plant Cell Physiol,50(7):1201-1214
Li C.X.,Li J.,Xiao L.T.,Guo L.,and Yu R.Q.,2003,Amperometric immunosensor based on mercapto carboxylic acid self-assembled monolayer for phytohormone indoleacetic acid assay,Huaxue Xuebao(Acta Chimica Sinica),61(5):790-794(李春香,李劲,萧浪涛,国励,俞汝勤,2003,基于巯基自组装单层膜的植物生长激素吲哚乙酸电化学免疫传感器的研究,化学学报,61(5):790-794)
Li C.X.,2003,Study on the photohormoneβ-Indole acetic acid electrochemical biosensor and other biosensor,Thesis for M.S.,Hunan University,Supervisor:Shen G.L.,pp.1-57(李春香,2003,植物生长激素β-吲哚乙酸等电化学生物传感器的研究,硕士学位论文,湖南大学,导师:沈国励,pp.1-57)
Li J.,Wu Z.Y.,Xiao L.T.,Zeng G.M.,Huang G.H.,Shen G.L.,and Yu R.Q.,2002,A novel piezoelectric biosensor for the detection of phytohormone,beta-indole acetic acid,Anal.Sci.,18(4):403-407
Li J.,Xiao L.T.,Zeng G.M.,Huang G.H.,Shen G.L.,and Yu R.Q.,2003,Amperometric immunosensor based on polypyrrole/poly(m-pheylenediamine)multilayer on glassy carbon electrode for cytokinin N 6-(△2-isopentenyl)adenosine assay,Anal.Biochem.,321(1):89-95
Li J.,2003,Studies on electrochemical biosensor techniques for endo-phytohormone determination,Dissertation for Ph.D.,Hunan University,Supervisor:Shen G.L.,pp.1-10(李劲,2003,植物内源激素等物质的电化学生物传感技术研究,博士学位论文,湖南大学,导师:沈国励,pp.1-10)
Li J.,Xiao L.T.,Zeng G.M.,Huang G.H.,Shen G.L.,and Yu R.Q.,2005,Immunosensor for rapid detection of gibberellin acid in the rice grain,J.Agric.Food Chem.,53(5):1348-1353
Li Y.W.,and Xiao L.T.,2007,A review on the recent status and development of phytohormonal analysis technology,Shengming Kexue Yiqi(Life Science Instruments),5(12):10-14(李雨薇,肖浪涛,2007,植物激素检测技术的现状和发展,生命科学仪器,5(12):10-14)
Liu X.,Ma L.,Lin Y.W.,and Lu Y.T.,2003,Determination of abscisic acid by capillary electrophoresis with laser-induced fluorescence detection,J.Chromatogr.A,1021(1):209-213
Lopez-Carbonell M.,Gabasa M.,and Jauregui O.,2009,Enhanced determination of abscisic acid(ABA)and abscisic acid glucose ester(ABA-GE)in Cistus albidus plants by liquid chromatography-mass spectrometry in tandem mode,Plant Physiol.Biochem.,47(4):256-261
Lu Q.M.,Zhang L.,Chen T.W.,Lu M.L.,and Chen G.N.,2009,Content change of endogenous plant hormones under salt stress by liquid chromatography-tandem mass spectrometry,Zhongguo Kexue(B Ji:Huaxue)(Science in China.Series B,Chemistry),39(8):785-792(卢巧梅,张兰,陈天文,卢明华,陈国南,2009,液相色谱-串联质谱分析盐胁迫下植物激素的含量变化,中国科学(B辑:化学),39(8):785-792)
Ma Y.N.,2011,Quantitative analysis of 37 phytohormones in Oryza sativa by high-performance liquid chromatography-mass spectrometry,Thesis for M.S.,Chinese Academy of Agricultural Sciences,Supervisor:Zhu Z.W.,pp.1-82(马有宁,2011,液相串联质谱测定水稻37种内源激素方法的研究,硕士学位论文,中国农业科学院,导师:朱智伟,pp.1-82)
Ma Z.,Ge L.,Lee A.S.,Yong J.W.H.,Tan S.N.,and Ong E.S.,2008,Simultaneous analysis of different classes of phytohormones in coconut(Cocos nucifera L.)water using highperformance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction,Anal.Chim.Acta,610(2):274-281
Maldiney R.,Leroux B.,Sabbagh I.,Sotta B.,Sossountzov L.,and Miginiac E.,1986,A biotin-avidin-based enzyme immunoassay to quantify three phytohormones:auxin,abscisic acid and zeatin-riboside,Journal of Immunological Methods,90(2):151-158
Mi J.,Zhou M.,Ding L.,Li Y.J.,Liu C.Y.,Ma Y.J.,and Chen H.,2011,Solid-phase extraction-flow injection chemiluminescence determination of indoleacetic acid in Arabidopsis,Fenxi Shiyanshi(Chinese Journal of Analysis Laboratory),30(8):44-47(米娟,周敏,丁兰,李玉杰,刘彩云,马永钧,陈慧,2011,固相萃取-化学发光法测定植物中的吲哚乙酸,分析试验室,30(8):44-47)
Muller A.,Duchting P.,and Weiler E.W.,2002,A multiplex GC-MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds,and its application to Arabidopsis thaliana,Planta,216(1):44-56
Muller M.,and Munne-Bosch S.,2011,Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry,Plant Methods,7(1):37
Nakajima M.,Yamaguchi I.,Nagatani A.,Kizawa S.,Murofushi N.,and Furuya M.,1991,Monoclonal antibodies specific for non-derivatized gibberellins I preparation of monoclonal antibodies against GA4 and their use in immunoaffmity column chromatography,Plant&Cell Physiology,32:515-521
Niu Q.,Zong Y.,Qian M.,Yang F.,and Teng Y.,2014,Simultaneous quantitative determination of major plant hormones in pear flowers and fruit by UPLC/ESI-MS/MS,Analytical Methods,6(6):1766-1773
Pan X.,and Wang X.,2009,Profiling of plant hormones by mass spectrometry,J.Chromatogr.B,877(26):2806-2813
Pan X.Q.,Welti R..,and Wang X.M.,2010,Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography-mass spectrometry,Nat.Protoc,5(6):986-992
Shankou W.S.Y.,Chen D.F.,and Chen B.S.,1989,Immunoassay of plant hormones,Shandong Linye Keji(Journal of Shandong Forestry Science and Technology),(2):68-74(山口五十鹰,陈得富,程炳篙,1989,植物激素的免疫测定,山东林业科技,(2):68-74)
Shen S.H.,and Zhu Z.Q.,1999,Advances in research of novel plant growth regulating substance-hormone like peptides',Zhiwu Xuebao(Chinese Bulletin of Botany),16(6):648-652(沈世华,朱至清,1999,新型植物生长调节物质-激素性多肤的研究进展,植物学报,16(6):648-652)
Tang S.G.,Xia Y.X.,and Pei Y.,1991,Quantitative analysis of plant hormones with indirect enzyme-linked immunosorent assay(ELISA),Xinandaxue Xuebao(Journal of Southwest Agricultural University),13(2):183-186(唐尚格,夏玉先,裴炎,1991,间接酶联免疫法测定植物内源激素,西南大学学报,13(2):183-186)
Tarkowska D.,Novak O.,Flokova K.,Tarkowski P.,Tureckova V.,Gruz J.,and Strnad M.,2014,Quo vadis plant hormone analysis?Planta,240(1):55-76
Tong J.H.,Li Y.W.,Huang Z.G.,Tian M.,and Ding J.H.,2009,Simultaneous determination of several phytohormones in cotton root by using high performance liquid chromatography,Xiandai Shengwuyixue Jinzhan(Progress in Modern Biomedicine),9(13):2476-2479(童建华,李雨薇,黄志刚,田梅,丁君辉,2009,高效液相色谱法同时检测棉花根中的多种植物激素,现代生物医学进展,9(13):2476-2479)
Von Schwartzenberg K.,Nunez M.F.,Blaschke H.,Dobrev P.I.,Novak O.,Motyka V.,and Strnad M.,2007,Cytokinins in the bryophyte Physcomitrella patens:analyses of activity,distribution,and cytokinin oxidase/dehydrogenase overexpression reveal the role of extracellular cytokinins,Plant Physiology,145(3):786-800
Wang B.Q.,and Niu J.S.,2007,A determination method for wheat grain gibberellins,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),23(1):334-337(王保勤,牛吉山,2007,一种小麦子粒赤霉素含量测定方法的建立,中国农学通报,23(1):334-337)
Wang L.,2014,Determination of plant hormones by matrix solid-phase dispersion-high performance liquid chromatography-tandem mass spectrometry,Dissertation for Ph.D.,University of Science and Technology of China,Supervisor:Guan Y.F.,pp.1-100(王璐,2014,基于基质固相分散萃取-高效液相色谱-串联质谱的植物激素分析,博士学位论文,中国科学技术大学,导师:关亚风,pp.1-100)
Wang Y.H.,Tan H.R.,Gao L.P.,Si X.Y.,and Shao L.,2008,Separation and determination of plant hormones by high-performance capillary electrophoresis,Jiguang Shengwu Xuebao(Acta Laser Biology Sinica),17(6):840-844(王艳红,檀华蓉,高丽萍,司雄元,邵黎,2008,高效毛细管电泳分离多种植物激素的方法研究,激光生物学报,17(6):840-844)
Weiler E.W.,1984,Immunoassay of plant growth regulators,Annual Review of Plant Physiology,35(1):85-95
Wu Q.,Wu D.,Duan C,Shen Z.,and Guan Y.,2012,Hollow fiber-based liquid-liquid-liquid micro-extraction with osmosis:Ⅱ.application to quantification of endogenous gibberellins in rice plant,J.Chromatogr.A,1265:17-23
Wu S.R.,Chen W.F.,and Zhou X.,1988,Enzyme linked immunosorbent assay for endogenous plant hormones,Zhiwu Shengli Xuebao(Plant Physiol.J.),5:53-57(吴颂如,陈婉芬,周燮,1988,酶联免疫法(EIISA)测定内源植物激素,植物生理学报,5:53-57)
Xu H.,Jia L.,and Liu Y.L.,2009,Determination of phytohormones in corns by capillary liquid chromatography,Fenxi Shiyanshi(Chinese Journal of Analysis Laboratory),28(2):30-33(徐卉,贾丽,柳亚玲,2009,毛细管液相色谱法分离植物内源激素,分析试验室,28(2):30-33)
Xue Q.H.,Li D.,and Tang L.,2000,Improvment of fluorescence method for the determination of gibberellin,Xibei Nongye Daxue Xuebao(Journal of Northwest Sci-Tech University of Agriculture and Forestry),28(4):34-39(薛泉宏,李东,汤莉,2000,赤霉素荧光测定法改进研究,西北农业大学学报,28(4):34-39)
Yin T.S.,Chen S.Y.,Yu Q.Y.,and Tang Y.H.,1990,Rapid spectrophotometriec determination of gibberellins,Fengxi Huaxue(Analytical Chemistry),18(10):966-969(印天寿,陈世勇,于群英,唐玉华,1990,赤霉素的快速分光光度测定法的研究,分析化学,18(10):966-969)
Yutaka M.,1968,Gibberellin-like activity of(-)-Kaurene,(-)-Kauren-19-ol and(-)-Kauren-19-oic acid in leaf sheath elongation of'Tan-ginbozu'dwarf of Oryza sativa,Botanical Magazine Tokyo,81(956):100-102
Zhang S.H.,Xi J.,and He Z.K.,2005,Chemiluminescence determination of indoleacetic acid with Ru(phen)_(2_+)~3-Ce(Ⅳ)system,Fengxi Kexue Xuebao(Journal of Analytical Science),21(4):387-389(张韶虹,郗娟,何治柯,2005,Ru(phen)_(2_+)~3-Ce(Ⅳ)化学发光体系检测吲哚乙酸的研究,分析科学学报,21(4):387-389)
Zhang X.C.,Shangguan Z.P.,and Gao S.M.,2005,Regulation mechanism of nitric oxide to plant growth and development,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),25(4):812-818(张绪成,上官周平,高世铭,2005,NO对植物生长发育的调控机制,西北植物学报,25(4):812-818)
Zhou Y.M.,and Xin X.,2011,Determination of plant hormone residues in vegetables and fruits by high performance liquid chromatography,Shipin Kexue(Food Science),31(18):301-304(周艳明,忻雪,2011,高效液相色谱法测定果蔬中7种植物激素的残留量,食品科学,31(18):301-304)
基本信息:
DOI：10.13417/j.gab.035.001258
中图分类号:Q946.885
引用信息:
[1]丁广文,李华,何桥.植物内源激素检测技术的研究进展[J].基因组学与应用生物学,2016,35(05):1258-1265.DOI:10.13417/j.gab.035.001258.
基金信息:
中央高校基本科研业务费专项资金(XDJK2015C164);; 重庆市基础科学与前沿技术研究(cstc2014jcyjA80006)共同资助
2016-05-25
2016-05-25