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连作障碍严重危害牡丹的生长发育,根际微生物环境可直接反映由连作障碍带来的变化,探究连作状态下不同种植年限‘凤丹’牡丹根际微生物环境的演替规律及菌体群落多样性的变化,对‘凤丹’牡丹产业可持续发展具有重要意义。本研究选用Illumina高通量测序技术,对洛阳地区2年、4年、5年、10年、32年生‘凤丹’牡丹根际真菌群落进行测序分析,研究真菌18S rDNA V5可变区的丰富度和多样性指数。测序后从5组不同种植年限根际土中共得到1 069 322条Clean reads,序列过滤拼接后可得534 659条Tags;5组土壤样品共含有3 419个OTUs,涵盖了5门、17纲、18目、175科、299属、376种菌群;科水平上‘凤丹’牡丹根际优势菌群为Incertae sedis (24.90%)、Sordariomycetes (15.25%)、Sordariaceae (12.11%)、Filobasidiales (7.48%)以及Rhizophlyctidaceae (6.21%);属水平上‘凤丹’牡丹根际优势菌群为Tetracladium (20.81%)、Incertae sedis(13.98%)、Sordaria (12.12%)和Filobasidiaceae (7.48%);Alpha多样性分析和Beta多样性分析均表明,不同种植年限‘凤丹’牡丹根际土菌群群落多样性为10年生>5年生>32年生>4年生>2年生。本研究发现连作状态下不同种植年限对‘凤丹’牡丹根际真菌群落多样性有显著影响,菌群群落多样性呈先升高后降低的趋势;不同种植年限中均具有特异性强的优势菌群;Gracilipodida、Acanthocystidae和Freshwater Opisthokonta三类真菌群落随种植年限的增加而出现,这些特异性菌群证明连作障碍对根际土壤真菌群落的影响。以上结果将为解析‘凤丹’牡丹连作障碍机制提供理论基础。
Abstract:The succession of tree peony continuous cropping has seriously jeopardized its growth and development.The rhizosphere soil microbial environment can directly reflect the changes brought about by continuous cropping obstacles. The change of community diversity has important theoretical significance for the sustainable development of the Paeonia ostii 'Feng Dan' industry. In this study, Illumina high-throughput sequencing technology was used to sequence and analyze the communities of 2-year-old, 4-year-old, 5-year-old, 10-year-old and 32-year-old Paeonia ostii 'Feng Dan' rhizosphere soil in Luoyang, and the richness and diversity of the variable region of the 18 S rDNA V5 were studied. After sequencing, We obtained 1 069 322 Clean reads in the rhizosphere soil of 5 different planting years, and 534 659 Tags were obtained after sequential filtration and splicing. 5 soil samples contained 3 419 OTUs, covering 5, 17 Classes, 18 orders, 175 families, 299 genera and 376 species of fungal flora; the predominant flora in the rhizosphere soil flora of Paeonia ostii 'Feng Dan' on the family level was:Incertae Sedis(24.90%), Sordariomycetes(15.25%), and Sordariaceae(12.11%), Filobasidiales(7.48%) and Rhizophlyctidaceae(6.21%); The dominant flora in the rhizosphere soil flora of Paeonia ostii 'Feng Dan' in the genus level was: Tetracladium(20.81%), Incertae Sedis(13.98%), Sordaria(12.12%) and Filobasidiaceae(7.48%).Alpha diversity analysis and Beta diversity analysis showed that the diversity of the flora in the rhizosphere soil of Paeonia ostii 'Feng Dan' in different planting years was: 10-year-old >5-year-old >32-year-old >4-year-old >2-year-old. This study frond that planting years had a significant effect on the microbial community diversity in rhizosphere soil of Paeonia ostii 'Feng Dan', and the community diversity of the bacterial community first increased and then decreased; The dominant microflora with strong specificity was found in different planting years. In the process of continuous cropping, the three fungal communities of Gracilipodida, Acanthocystidae and Freshwater Opisthokonta only appeared with the increase of planting time. These specific bacterial groups can directly explain the effect of continuous cropping obstacles on rhizosphere soil fungal communities. The above results will provide a theoretical basis for the analysis of the continuous barrier mechanism of Paeonia ostii 'Feng Dan'.
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基本信息:
DOI:10.13417/j.gab.039.001672
中图分类号:S565.9;S154.3
引用信息:
[1]李昱莹,刘曙光,廉小芳,等.油用牡丹‘凤丹’不同种植年限根际真菌群落多样性变化研究[J].基因组学与应用生物学,2020,39(04):1672-1685.DOI:10.13417/j.gab.039.001672.
基金信息:
河南省高等学校重点科研项目(19A180002);; 国家自然科学基金项目(U1804233)
2019-03-19
2019-03-19
2019-03-19