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2017, 05, v.36 1935-1945
长江水系中华绒螯蟹野生和人工繁殖大眼幼体的遗传多样性分析
基金项目(Foundation): 上海市科委工程技术中心能力提升项目(No.16DZ2281200);; 崇明县科技攻关项目(No.CK2015-21);; 上海高校水产学高峰学科建设项目(No.2015-62-0908)共同资助
邮箱(Email):
DOI: 10.13417/j.gab.036.001935
发布时间: 2017-05-25
出版时间: 2017-05-25
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摘要:

长江水系中华绒螯蟹是我国最重要的河蟹养殖群体,通常认为长江野生大眼幼体在成蟹阶段具有一定的生长优势,但是长江流域河蟹增殖放流和养殖群体逃逸可能对其野生大眼幼体遗传多样性造成一定的影响,尚未见相关报道。因此,本研究利用29对微卫星标记对河蟹长江水系野生和人工繁殖大眼幼体的亲缘关系及遗传多样性进行了分析。两群体大眼幼体均表现出较高的杂合水平,两个种群Ho分别为0.729 9和0.750 0,无显著差异(p>0.05)。两群体总近交系数(Fit)为0.171 2,群体内近交系数(Fis)为0.165 9,群体间分化系数(Fst)为0.006 4;分子方差分析(AMOVA)显示,99.7%的遗传变异来自种群内个体间,0.3%来源于种群间的变异,遗传分化程度较低;各位点的基因流Nm平均值为38.739 9,说明两种群基因交流频繁;群间平均遗传距离仅为0.106 7。瓶颈效应和遗传结构分析显示,两个群体近期经历过瓶颈效应,群体间间遗传混杂,不能分出有效理论种群数。综上所述,长江口河蟹野生大眼幼体的SSR遗传多样性稍高于人工繁殖群体,两者的遗传分化程度较低,这暗示两群体间存在基因交流。

Abstract:

The Chinese mitten crab of Yangtze River is the most important breeding population in China. In general, the wild megalopa from the Yangtze River has a growth advantage in the culture stage of adult E. sinensis,while the artificial propagation and releasing of cultured E. sinensis may cause a certain impact on the genetic diversity of wild population of megalopa, but there are limited available information. Therefore, this study selected29 microsatellite locus with high polymorphism for the analysis of genetic relationship and genetic diversity of the wild and cultured megalopa of E. sinensis. Two populations both showed high levels of heterozygosity(Ho=0.729 9and 0.750 0) with no significant difference(p>0.05); the total inbreeding coefficient(Fit) of the two populations was 0.171 2, the inbreeding coefficient(Fis) was 0.165 9, and the populations differentiation factor(Fst) was 0.006 4;analysis of molecular variance(AMOVA) implied that 99.7% of the genetic variation was within population,0.3% variati on was between populations, and the genetic differentiation was low; the average gene flow(Nm)value of each site was 38.739 9, which indicated that two groups exchanged genes frequently; average genetic distance between the groups was only 0.106 7; bottleneck effect and genetic structure analysis revealed that the two groups experienced a bottleneck effect in recent years, and the genetic diversity among populations could not distinguish the effective population. In conclusion, SSR genetic diversity of wild megalopa in Yangtze River was higher than that of the cultured populations, and the genetic differentiation of the two was low, suggesting that there was genetic communication between the two populations.

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基本信息:

DOI:10.13417/j.gab.036.001935

中图分类号:S917.4

引用信息:

[1]肖起珍,刘青,吴旭干,等.长江水系中华绒螯蟹野生和人工繁殖大眼幼体的遗传多样性分析[J].基因组学与应用生物学,2017,36(05):1935-1945.DOI:10.13417/j.gab.036.001935.

基金信息:

上海市科委工程技术中心能力提升项目(No.16DZ2281200);; 崇明县科技攻关项目(No.CK2015-21);; 上海高校水产学高峰学科建设项目(No.2015-62-0908)共同资助

发布时间:

2017-05-25

出版时间:

2017-05-25

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