| 679 | 7 | 119 |
| 下载次数 | 被引频次 | 阅读次数 |
本研究利用分子标记技术，分析所收集的澳洲坚果(Macadamia spp.)种质的遗传多样性，构建其指纹图谱，旨在为澳洲坚果遗传资源保护、良种选育和品种鉴定提供技术保障。基于转录组测序结果，开发出了12个特异性强、多态性高的表达序列标签-简单重复序列(expressed sequence tags-simple sequence repeat, EST-SSR)分子标记。对51份澳洲坚果种质进行SSR荧光标记毛细管电泳检测，分析种质的遗传多样性，进行系统聚类及指纹图谱构建。结果表明，12对EST-SSR分子标记引物共检测到72个等位基因，平均每对引物扩增出6个等位基因，多态性信息含量(polymorphic information content, PIC)平均值为0.632,标记多态性高。在遗传相似系数为0.422时，可以将51份澳洲坚果种质分成6类；采用引物代码和多态性片段长度组合法构建了51份澳洲坚果种质的分子指纹图谱。本研究新开发的12个EST-SSR分子标记，可用于澳洲坚果种质资源的亲缘关系分析和品种鉴定。
Abstract：In this study, the genetic diversity of Macadamia spp. germplasm collected was analyzed by molecular marker technology, and construct the fingerprint, so as to provide technical support for the protection of macadamia genetic resources research, breeding of improved varieties and variety identification of macadamia. Based on the results of transcriptomic sequencing, 12 expressed sequence tags-simple sequence repeat(EST-SSR) molecular markers with strong specificity and high polymorphism were deve-loped. 51 macadamia germplasm samples were detected by capillary electrophoresis with SSR fluorescent markers to analyze the genetic diversity of germplasm, perform systematic clustering and construct fingerprint. The results showed that a total of 72 alleles were detected by 12 pairs of EST-SSR primers, and an average of 6 alleles were amplified from each primers. The average polymorphic information content(PIC) was 0.632, indicating high polymorphism of these EST-SSR markers. When the genetic similarity coefficient was 0.422, 51 macadamia germplasms could be divided into 6 categories. The molecular fingerprints of 51 macadamia germplasms were constructed by using primer code and polymorphism fragment length. In this study, 12 EST-SSR markers were developed, which could be used for genetic relationship analysis and variety identification of macadamia germplasm resources.
陈晓明，李魁鹏，陈博雯，等，2018.马尾松转录组SSR序列特征分析及其分子标记开发.分子植物育种，16(19):6407-6414.[CHEN X M,LI K P,CHEN B W,et al.,2018.Characterization analysis of SSR sequences in the transcriptome of Pinus massoniana and its molecular marker development.Molecular Plant Breeding,16(19):6407-6414.]
陈兴彬，何龙燕，肖复明，等，2020.基于转录组测序的陈山红心杉EST-SSR开发及应用.中南林业科技大学学报，40(8):120-127.[CHEN X B,HE L Y,XIAO F M,et al.,2020.Development and application of EST-SSR markers in Chenshan red-heart Chinese fir based on transcriptome sequencing.Journal of Central South University of Forestry & Technology,40(8):120-127.]
邓绍勇，祝必琴，李康琴，等，2022.基于EST-SSR标记的栀子品种亲缘关系分析及指纹图谱构建.中草药，53(9):2795-2802.[DENG S Y,ZHU B Q,LI K Q,et al.,2022.Genetic relationship analysis and fingerprint construction of Gardenia jasminoides cultivars based on EST-SSR markers.Chinese Traditional and Herbal Drugs,53(9):2795-2802.]
费勤娇，丁洪昌，张鹏，等，2021.基于转录组信息的坛紫菜EST-SSR标记的开发及新品系SW-81的种质鉴定.上海海洋大学学报，30(3):464-474.[FEI Q J,DING H C,ZHANG P,et al.,2021.Development of EST-SSR marker based on transcriptome and the research of germplasm identification for the new strain (SW-81) of Pyropia haitanensis.Journal of Shanghai Ocean University,30(3):464-474.]
高源，王昆，王大江，等，2016.利用TP-M13-SSR标记构建苹果栽培品种的分子身份证.园艺学报，43(1):25-37.[GAO Y,WANG K,WANG D J,et al.,2016.Molecular ID establishment of apple cultivars by TP-M13-SSR.Acta Horticulturae Sinica,43(1):25-37.]
郭俊，朱婕，谢尚潜，等，2020.油梨转录组SSR分子标记开发与种质资源亲缘关系分析.园艺学报，47(8):1552-1564.[GUO J,ZHU J,XIE S Q,et al.,2020.Development of SSR molecular markers based on transcriptome and analysis of genetic relationship of germplasm resources in avo-cado.Acta Horticulturae Sinica,47(8):1552-1564.]
郭艳春，张力岚，陈思远，等，2021.黄麻应用核心种质的DNA分子身份证构建.作物学报，47(1):80-93.[GUO Y C,ZHANG L L,CHEN S Y,et al.,2021.Establishment of DNA molecular fingerprint of applied core germplasm in jute(Corchorus spp.).Acta Agronomica Sinica,47(1):80-93.]
韩志强，任勇谕，夏宇飞，等，2019.毛白杨多态SSR引物库和种质资源指纹图谱库构建.北京林业大学学报，41(7):10-18.[HAN Z Q,REN Y Y,XIA Y F,et al.,2019.Construction of polymorphic SSR primer library and germplasm resource fingerprint database of Populus tomentosa.Journal of Beijing Forestry University,41(7):10-18.]
李冰，杨祥燕，蔡元保，等，2022.分子标记在澳洲坚果遗传育种中的应用研究进展.农业研究与应用，35(4):39-43.[LI B,YANG X Y,CAI Y B,et al.,2022.Research progress on application of molecular marker technology to Macadamia genetic breeding.Agricultural Research and App-lication,35(4):39-43.]
李益，马先锋，唐浩，等，2018.柑橘品种鉴定的SSR标记开发和指纹图谱库构建.中国农业科学，51(15):149-159.[LI Y,MA X F,TANG H,et al.,2018.SSR mar-kers screening for identification of citrus cultivar and construction of DNA fingerprinting library.Scientia Agricultura Sinica,51(15):149-159.]
李志强，贺熙勇，吴超，等，2018.分子标记在澳洲坚果研究中的应用.热带农业科技，41(2):46-49.[LI Z Q,HE X Y,WU C,et al.,2018.Application of molecular marker techniques in Macadamia research.Tropical Agricultural Science & Technology,41(2):46-49.]
李志强，吴超，贺熙勇，等，2022.基于SSR标记的澳洲坚果种质资源DNA指纹图谱的构建.果树学报，39(11):2028-2035.[LI Z Q,WU C,HE X Y,et al.,2022.Construction of DNA fingerprint of macadamia germplasm based on SSR markers.Journal of Fruit Science,39(11):2028-2035.]
梁燕，韩传明，周继磊，等，2022.山东核桃良种SSR指纹图谱及分子身份证的构建：基于毛细管电泳分析.中国农学通报，38(15):113-121.[LIANG Y,HAN C M,ZHOU J L,et al.,2022.Construction of SSR fingerprint and molecular identity card of Shandong elite Julans regia cultivars:based on capillary electrophoresis analysis.Chinese Agricultural Science Bulletin,38(15):113-121.]
毛秀红，郑勇奇，孙百友，等，2017.基于SSR的刺槐无性系遗传多样性分析和指纹图谱构建.林业科学，53(10):80-89.[MAO X H,ZHENG Y Q,SUN B Y,et al.,2017.Genetic diversity and fingerprints of Robinia pseudoacacia clones based on SSR markers.Scientia Silvae Sinicae,53(10):80-89.]
任二芳，牛德宝，刘功德，等，2020.澳洲坚果仁营养成分分析与其加工副产物的综合利用研究.食品研究与开发，41(6):194-199.[REN E F,NIU D B,LIU G D,et al.,2020.Study on analysis of nutritional components of Macadamia nuts and comprehensive utilization of its processing by-products.Food Research and Development,41(6):194-199.]
任重，汪贵斌，杨晓明，等，2020.基于SSR分子标记的枸杞遗传多样性研究.经济林研究，38(3):95-103.[REN Z,WANG G B,YANG X M,et al.,2020.Genetic diversity of Chinese wolfberry (Lycium ruthenicum L.) based on SSR molecular markers.Non-wood Forest Research,38(3):95-103.]
时小东，朱学慧，盛玉珍，等，2016.基于转录组序列的楠木SSR分子标记开发.林业科学，52(11):71-78.[SHI X D,ZHU X H,SHENG Y Z,et al.,2016.Development of SSR markers based on transcriptome sequence of Phoebe zhennan.Scientia Silvae Sinicae,52(11):71-78.]
宋家明，李欣窈，张诗慧，等，2022.基于转录组数据的海南油茶SSR分子标记的开发与评价.分子植物育种，20(20):6791-6801.[SONG J M,LI X Y,ZHANG S H,et al.,2022.Development and evaluation of SSR markers based on transcriptome sequencing of Camellia in Hainan Province.Molecular Plant Breeding,20(20):6791-6801.]
孙泽硕，2022.基于表型性状和SSR标记的樱花品种遗传多样性分析及指纹图谱构建，硕士学位论文.郑州：河南农业大学.[SUN Z S,2022.Genetic diversity analysis and fingerprint construction of oriental cultivars of flowering cherry based on phenotypic traits and SSR makers,Thesis for M.S.Zhengzhou:Henan Agricultural University.]
位明明，黄肖，李维国，等，2022.利用SSR荧光标记构建37个中国橡胶树栽培品种指纹图谱.热带作物学报，43(8):1565-1576.[WEI M M,HUANG X,LI W G,et al.,2022.Using the SSR fluorescent labeling to establish SSR fingerprints for 37 cultivars in rubber tree(Hevea brasiliensis).Chinese Journal of Tropical Crops,43(8):1565-1576.]
魏秀清，李亮，熊亚庆，等，2022.基于SSR分子标记的福建百香果品种(系)鉴定及指纹图谱构建.福建农业学报，37(10):1288-1297.[WEI X Q,LI L,XIONG Y Q,et al.,2022.Identification and fingerprint construction of Fujian passion fruit varieties (lines) based on SSR molecular markers.Fujian Journal of Agricultural Sciences,37(10):1288-1297.]
余兴华，杨艳梅，李玉祥，等，2022.基于八角转录组序列的SSR分子标记引物开发.西南农业学报，35(6):1261-1268.[YU X H,YANG Y M,LI Y X,et al.,2022.Development of SSR molecular markers based on transcriptome sequencing of Illicium verum.Southwest China Journal of Agricultural Sciences,35(6):1261-1268.]
张利达，唐克轩，2010.植物EST-SSR标记开发及其应用.基因组学与应用生物学，29(3):534-541.[ZHANG L D,TANG K X,2010.Development of plant EST-SSR markers and its application.Genomics and Applied Biology,29(3):534-541.]
张振，张含国，莫迟，等，2015.红松转录组SSR分析及EST-SSR标记开发.林业科学，51(8):114-120.[ZHANG Z,ZHANG H G,MO C,et al.,2015.Transcriptome sequencing analysis and development of EST-SSR markers for Pinus koraiensis.Scientia Silvae Sinicae,51(8):114-120.]
朱阅，郭亚静，冯程，等，2021.星油藤转录组SSR序列特征分析及其分子标记开发.分子植物育种，19(9):2985-2992.[ZHU Y,GUO Y J,FENG C,et al.,2021.Characteristic analysis of SSR sequences and development of molecular markers based on Plukenetia volubilis transcriptome.Molecular Plant Breeding,19(9):2985-2992.]
ARNAUD-HAOND S,STOECKEL S,BAILLEUL D,2020.New insights into the population genetics of partially clonal organi-sms:when seagrass data meet theoretical expectations.Mol.Ecol.,29(17):3248-3260.
BOTSTEIN D,WHITE R L,SKOLNICK M,et al.,1980.Construction of a genetic linkage map in man using restriction fragment length polymorphisms.Am.J.Hum.Genet.,32(3):314-331.
HARDNER C M,PEACE C,LOWE A J,et al.,2009,Genetic resources and domestication of macadamia,Hortic.Rev.,USA:33.
KALIA R K,RAI M K,KALIA S,et al.,2011.Microsatellite markers:an overview of the recent progress in plants.Euphytica,177(3):309-334.
LI T Q,LIU X F,LI Z H,et al.,2018.Development of novel EST-SSR markers for Rhododendron longipedicellatum (Ericaceae) and cross-amplification in two congeners.Appl.Plant Sci.,6(6):e01162.
LIN J S,ZHANG W P,ZHANG X T,et al.,2022.Signatures of selection in recently domesticated macadamia.Nat.Commun.,13(1):242.
LU C R,ZOU C S,ZHANG Y P,et al.,2015.Development of chromosome-specific markers with high polymorphism for allotetraploid cotton based on genome-wide characterization of simple sequence repeats in diploid cottons (Gossypium arboreum L.and Gossypium raimondii Ulbrich).BMC Genom.,16(1):55.
MACHADO NETO N B,MORYIA A T,2010.Variability in Macadamia integrifolia by RAPD markers.Crop Breed.Appl.Biotechnol.,10(3):266-270.
NIU Y F,LI G H,NI S B,et al.,2022.The chromosome-scale reference genome of Macadamia tetraphylla provides insights into fatty acid biosynthesis.Front Genet,13:835363.
NOCK C J,BATEN A,MAULEON R,et al.,2020.Chromosome-scale assembly and annotation of the Macadamia genome (Macadamia integrifolia HAES 741).G3 (Bethesda).,10(10):3497-3504.
PEACE C P,ALLAN P,VITHANAGE V,et al.,2005.Gene-tic relationships amongst macadamia varieties grown in South Africa as assessed by RAF markers.S.Afr.J.Plant Soil,22(2):71-75.
PEAKALL R,SMOUSE P E,2012.GenAlEx 6.5:genetic analy-sis in Excel.Population genetic software for teaching and research:an update.Bioinform.(Oxf.Engl.),28(19):2537-2539.
RANKETSE M,HEFER C A,PIERNEEF R,et al.,2022.Genetic diversity and population structure analysis reveals the unique genetic composition of South African selected macadamia accessions.Tree Genet.Genomes,18(2):1-12.
R?DER M,WENDEHAKE K,KORZUN V,et al.,2002.Construction and analysis of a microsatellite-based database of European wheat varieties.Theor.Appl.Genet.,106(1):67-73.
SCOTT K D,EGGLER P,SEATON G,et al.,2000.Analysis of SSRs derived from grape ESTs.Theor.Appl.Genet.,100(5):723-726.
SHARMA P,MURIGNEUX V,HAIMOVITZ J,et al.,2021.The genome of the endangered Macadamia jansenii displays little diversity but represents an important genetic resource for plant breeding.Plant Direct,5(12):e364.
SONG Y P,JIANG X B,ZHANG M,et al.,2012.Differences of EST-SSR and genomic-SSR markers in assessing genetic diversity in poplar.For.Stud.China,14(1):1-7.
STEIGER D L,MOORE P H,ZEE F,et al.,2003.Genetic relationships of macadamia cultivars and species revealed by AFLP markers.Euphytica,132(3):269-277.
STEPHENSON R,2005,Macadamia:domestication and commercialization,Chronica Horticulture,45(2):11-15.
UNTERGASSER A,CUTCUTACHE I,KORESSAAR T,et al.,2012.Primer3:new capabilities and interfaces.Nucleic.Acids Res.,40(15):e115.
WANG P,MO Y,WANG Y,et al.,2022.Macadamia germplasm and genomic database (MacadamiaGGD):a comprehensive platform for germplasm innovation and functional genomics in Macadamia.Front.Plant Sci.,13:1007266.
WU J,CAI C F,CHENG F Y,et al.,2014.Characterisation and development of EST-SSR markers in tree peony using transcriptome sequences.Mol.Breed.,34(4):1853-1866.
XIA C,JIANG S,TAN Q,et al.,2022.Chromosomal-level genome of macadamia (Macadamia integrifolia),Tropical Plants,1:1-9.
基本信息:
DOI：10.13417/j.gab.042.001172
中图分类号:S664
引用信息:
[1]黄健婷,莫怿,陶亮,等.基于EST-SSR分子标记的澳洲坚果分子指纹图谱构建[J].基因组学与应用生物学,2023,42(11):1172-1188.DOI:10.13417/j.gab.042.001172.
基金信息:
广西大学科研启动基金项目(A3360051008,A3360051020)资助
2023-03-16
2023-03-16
2023-03-16