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山葵树叶中含有丰富的矿物质和维生素,体外研究证实山葵树叶提取物具有较好的体外抗氧化活性。然而,山葵树叶提取物在体内的抗氧化和抗疲劳作用尚不明确。本研究分别按照100 mg/kg·bw、200 mg/kg·bw和500 mg/kg·bw的剂量对SD大鼠灌胃山葵树叶提取液4周。研究显示,山葵树叶提取物可以剂量依赖性方式提高大鼠的力竭游泳时间(p<0.05)。山葵树叶提取物可显著降低力竭游泳大鼠血清Lac和BUN水平并升高NEFA水平。山葵树叶提取物可提高大鼠的肝糖原和肌糖原含量。山葵树叶提取物可提高大鼠血清和组织SOD、GSH-Px和CAT水平并降低MDA水平。山葵树叶提取物可上调大鼠肝组织中ORM1、CCR5的m RNA和蛋白水平。本研究表明山葵树叶提取物可有效提高力竭游泳大鼠的抗疲劳能力。山葵树叶提取物的抗疲劳作用与减少乳酸堆积、增加脂肪动员、减少糖原分解、提高抗氧化活性有关。此外,山葵树叶提取物的抗疲劳作用与上调ORM1和CCR5的表达有关。
Abstract：The leaves of Moringa oleifera Lam. are rich in minerals and vitamins. In vitro studies have confirmed that the extract of Moringa oleifera Lam. leaves has good antioxidant activity in vitro. However, the anti-oxidation and antifatigue effects of Moringa oleifera Lam. leaf extract in vivo are not clear. In this study, SD rats were orally administered with Moringa oleifera Lam. leaf extract for 4 weeks at a dose of 100 mg/kg·bw, 200 mg/kg·bw, 500 mg/kg·bw.Studies showed that the extract of Moringa oleifera Lam. leaves increased the exhaustive swimming time of rats in a dose-dependent manner(p<0.05). Moringa oleifera Lam. leaf extract significantly reduced serum Lac and BUN levels and increased NEFA levels in exhausted swimming rats. Moringa oleifera Lam. leaf extract increased liver glycogen and muscle glycogen content in rats. Moringa oleifera Lam. leaf extract increased serum and tissue SOD, GSH-Px and CAT levels and decreased MDA levels. Moringa oleifera Lam. leaf extract up-regulated the m RNA and protein levels of ORM1 and CCR5 in rat liver tissue. This study shows that the extract of Moringa oleifera Lam. leaves can effectively improve the anti-fatigue ability of exhausted swimming rats. The anti-fatigue effect of Moringa oleifera Lam. leaf extract is related to reducing lactic acid accumulation, increasing fat mobilization, reducing glycogen decomposition, and increasing antioxidant activity. In addition, the anti-fatigue effect of Moringa oleifera Lam. leaf extract is related to up-regulation of ORM1 and CCR5 expression.
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基本信息:
DOI：10.13417/j.gab.039.000921
中图分类号:R285.5
引用信息:
[1]孟书樵,韩姗姗.山葵树叶提取物在力竭运动大鼠中的抗疲劳机制研究[J].基因组学与应用生物学,2020,39(02):921-928.DOI:10.13417/j.gab.039.000921.
2020-02-25
2020-02-25