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白花除虫菊(Tanacetum cinerariifolium)是全球唯一集约化种植的杀虫园艺作物，从其头状花序提取的天然除虫菊酯广泛应用于有机农业和家居害虫防控，具有极高的商业价值。本研究通过Illumina HiSeq 2500测序平台对白花除虫菊叶绿体基因组进行了测序，并对其结构特征及系统进化关系进行了分析。结果表明，白花除虫菊叶绿体基因组为典型的四分体结构，基因组大小为150 171 bp,包括84个蛋白质编码基因、 37个转运RNA基因和8个核糖体RNA基因；密码子偏好性分析发现其叶绿体基因组中密码子末端偏好以A和U结尾，其中亮氨酸的密码子是最常用的，其使用UUA密码子的频率最高，RSCU值为1.88。在其叶绿体基因组中鉴定到简单重复序列(simple sequence repeat, SSR)位点共41个，包含37个单核苷酸重复序列、 4个复合型核苷酸重复序列；基于菊科(Asteraceae)39个叶绿体基因组序列构建的系统发育树表明，其叶绿体基因组高度保守，除近缘红花除虫菊(T.coccineum)外，除虫菊类与同为菊科的植物蒿子杆(Ismelia carinata)亲缘较近。本研究结果能为白花除虫菊分子标记的开发、遗传多样性，以及为进一步研究菊蒿属(Tanacetum)植物的保护生物学和种群遗传学奠定基础。
Abstract：Tanacetum cinerariifolium is the sole insecticidal horticultural crop cultivated commercially worldwide due to its high commercial value of natural pyrethrins extracted from flower heads, widely used in organic agriculture and household pest control. In this study, the chloroplast genome of T. cinerariifolium was sequenced using the Illumina HiSeq 2500 sequencing platform, and its structural features and systematic evolution were analyzed. The results showed that T. cinerariifolium has a typical chloroplast quadripartite structure with a genome size of 150 171 bp, including 84 protein-coding genes, 37 transfer RNA genes and 8 ribosomal RNA genes. Codon preference analysis revealed an A/U-bias at the third codon position in its chloroplast genome, with the codon for leucine being the most frequently used. A total of 41 simple sequence repeat(SSR) loci were identified in its chloroplast genome, including 37 single nucleotide repeats and 4 compound nucleotide repeats. Based on a phylogenetic tree constructed from the chloroplast genome sequences of 39 Asteraceae species, the chloroplast genome of T. cinerariifolium was highly conserved, and it showed a close relationship with Ismelia carinata, except for its closely related Tanacetum coccineum. These findings could provide a basis for developing molecular markers, genetic diversity, and further studies on the conservation biology and population genetics of Tanacetum.
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基本信息:
DOI：10.13417/j.gab.043.000063
中图分类号:S68
引用信息:
[1]吕薇,付瀚森,李伽文,等.白花除虫菊叶绿体基因组特征及系统发育研究[J].基因组学与应用生物学,2024,43(01):63-77.DOI:10.13417/j.gab.043.000063.
基金信息:
国家自然科学基金项目(32160718); 贵州省科技计划一般项目[ZK[2022]301]; 黔师新苗[2022]19号; 贵州省教育厅普通高等学校青年科技人才成长项目[KY[2022]170]共同资助
2023-09-14
2023-09-14
2023-09-14