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为探究ATP合酶F0部分的orfB基因与植物雄性不育性的关系,本实验以3个烟草雄性不育系(MS中烟90,ms云烟85和MSK326)及其同型保持系为供试材料,提取其花蕾总DNA,利用PCR特异引物扩增目的基因orfB,并对目的基因进行测序和对比分析。结果显示:3个保持系的orfB基因序列完全一致,且与GenBank数据库中收录的烟草orfB基因序列吻合;3个不育系的目的基因序列也完全一致,但与保持系相比不育系目的基因在第363碱基位点发生A→C的突变,导致该位点密码子编码的Lue(亮氨酸)变异为Phe(苯丙氨酸)。因此推测烟草的orfB基因可能与其雄性不育性的形成有关。
Abstract：To research the correlation between subunit gene orfB of F0-ATPase and plant male sterility,three different species of tobacco male sterility lines(MS Zhongyan 90,MS Yunyan85,MS K326) and their corresponding maintainer lines were worked as tested materials in the experiment.Extracting the total DNA of the bud,the experiment used PCR specific primer to amplify target gene orfB,What's more,sequencing and contrastive analysis were carried out to the target gene.The results showed:orfB gene sequences of maintainer lines were on all fours in the three tested materials,and they were consistent with orfB gene sequences of tobacco that were included in the GenBank database.As well,target gene sequences of male sterility lines were in full accord,but at the side of the maintainer lines,the 363 base sites in orfB gene sequence of male sterility lines were changed,in particular,the change of base A→C site turned Lue(leccine) that was encoded by the codon in 363 base site into Phe(phenylalanine).Therefore,it can infer that tobacco orfB gene may be connected with the form of cytoplasmic male sterility in tobacco.
Ba Q.S.,Zhang G.S.,Li G.P.,and Fu Z.L.,2015,Research advances on the relationship between mitochondrion and cytoplasmic male sterility in wheat,Mailei Zuowu Xuebao(Journal of Triticeae Crops),35(1):16-21(巴青松,张改生,李桂萍,傅兆麟,2015,小麦线粒体与细胞质雄性不育关系的研究进展,麦类作物学报,35(1):16-21)
Bergman P.,Edqvist J.,Farbos I.,and Glimelius K.,2000,Male-sterile tobacco displays abnormal mitochondrial atpl transcript accumulation and reduced floral ATP/ADP ratio,Plant Molecular Biology,42(3):531-544
Das S.,Sen S.,Chakraborty A.,Chakraborti P.,Maiti M.,Basu A.,Basu D.,and Sen S.,2010,An unedited 1.1kb mitochondrial orfB gene transcript in the Wild Abortive Cytoplasmic Male Sterility(WA-CMS)system of Oryza sativa L.subsp.Indica,Bmc.Plant Biology,10(1):76-78
Dieterich J.H.,Braun H.P.,and Schmitz U.K.,2003,Alloplasmic male sterility in Brassica napes(CMS'Toumefortii-Stiewe')is associated with a special gene arrangement around a nove1 atp9 gene,Molecular Genetics and Genomics,269(6):723-731
Duan J.Q.,Du G.H.,Li J.Y.,Liang X.N.,and Liu F.H.,2008,Cloning and expression of atp6 and atp9 genes from ramie(boehmeria nivea(L.)Gaud.)and their relation ship with cytoplasmic male sterility,Yichuan(Hereditas),30(11):1487-1498)(段继强,杜光辉,李建永,梁雪妮,刘飞虎,2008,苎麻atp6和atp 9基因的克隆表达及与细胞质雄性不育的相关性,遗传,30(11):1487-1498)
Han Y.F.,Zhang L.Y.,Hu J.M.,Zhang G.S.,Li Y.X.,Sheng Y.,Wei F.,Niu N.,and Ma S.C.,2010,Editing sites in transcript of mitochondrial atp6 gene of male sterile line with Aegilops kotschyi cytoplasm in wheat,Zuowu Xuebao(Acta Agronomica Sinica),36(12):2179-2184(韩艳芬,张龙雨,胡俊敏,张改生,李亚鑫,盛英,位芳,牛娜,马守才,2010,黏类小麦细胞质雄性不育线粒体atp 6基因转录本编辑位点作物学报,36(12):2179-2184)
Han Y.Y.,Ma Y.Q.,Li D.Z.,and Xu Z.Q.,2015,The influences of Arabidopsis Pri-miRl56a on flowering time of Nicotiana tabacum,Jiyinzuxue yu Yingyong Shengwuxue(Genomics and Applied Biology),34(2):350-356(韩瑶瑶,马燕勤,李典珍,徐子勤,2015,拟南芥Pri-miR156a基因对烟草开花时间的影响,基因组学与应用生物学,34(2):350-356)
Heazlewood J.L.,Whelan J.,and Millar A.H.,2003,The prodnets of the mitochondrial orf25 and orfB genes are F_0 components in the plant F,Fo-ATP synthase,FEBS Letters,540(1):201-205
Hou J.H.,Tian Z.H.,He X.Q.,Zhang H.Z.,and Shang J.W.,2003,Difference of some physiological property of cytoplasmic male sterile line and maintainer line in sugerbeet,Huabei Nongxuebao(Acta Agriculture Boriali-sinica),18(3):32-35(侯建华,田自华,贺学勤,张惠忠,邵金旺,2003,甜菜胞质雄性不育系与保持系某些生理特性的差异,华北农学报,18(3):32-35)
Kim D.H.,and Kim B.D.,2006,The organization of mitochondrial atp6 gene region in male fertile and CMS lines of pepper(Capsicum annuum L.),Current Genetics,49(1):59-67
Liu Q.Y.,Zhu T.Y.,Zhao T.,and Huang H.Q.,2009,Aberrant mitochondrial gene ORF25 may cause tobacco male sterility,Jiangxi Nongye Daxue Xuebao(Acta Agriculturae Universitatis Jiangxiensis),31(1):54-57(刘齐元,朱滕义,赵婷,黄海泉,2009,异常线粒体基因ORF25可能导致烟草雄性不育,江西农业大学学报,31(1):54-57)
Lv J.H.,and Deng M.H.,2014,Relationship between male sterility and energy metabolism in plants,Lajiao Zazhi(Journal of China Capsicum),12(1):1-6(吕俊恒,邓明华,2014,植物雄性不育与能量代谢的关系,辣椒杂志,12(1):1-6)
LV Y.W.,Zhang Y.J.,Lv Y.W.,Wang R J.,Yang Z.S.,and Wang Y.J.,2016,Transformation of Mytilus gallop rovincialis foot protein type 5(Mgfp-5)gene in tobacco,Jiyinzuxue yu Yingyong Shengwuxue(Genomics and Applied Biology),35(1):172-182(吕玉伟,张雨靖,吕亚维,王睿劫,杨泽嫡,王英娟,2016,贻贝粘蛋白Mgfp-5基因在烟草中的转化,基因组学与应用生物学,35(1):172-182)
Mohr S.,Schulte-Kappert E.,Odenbach W.,Oettler G.,and Kuck U.,1993,Mitochondrial DNA of cytoplasmic male-sterile Triticum timopheevi:rearrangement of upstream sequences of the atp6 and orf25 genes,Theoretical and Applied Genetics,86(2):259-268
Mouras A.,Suharsono.,Hemould M.,Zabaleta A.,and Araya A.,1999,Inheritance of an induced male-sterile trait in transgenic plants expressing an engineered unedited atp9 mitochondrial gene,Theor.Appl.Genet.,98(3-4):614-621
Nakajima Y.,Yamamoto T.,Muranaka T.,and Oeda K.,2001,A novel orfB-related gene of carrot mitochondrial genomes that is associated with homeotic cytoplasmic male sterility(CMS),Plant Molecular Biology,46(1):99-107
Sabar M.,Gagliardi D.,Balk J.,and Leaver C.J.,2003,ORFB is a subunit of F_1F_0-ATP synthase:insight into the basis of cytoplasmic male sterility in sunflower,EMBO Reports,4(4):381-386
Sabar M.,Paepe R.D.,and Kouchkovsky Y.,2000,ComplexⅠimpairment,respiratory compensations,and photosynthetic decrease in nuclear and mitochondrial male sterile mutants of Nicotiana sylvestris,Plant Physiology,124(3):1239-1250
Shi Z.,Cheng D.Y.,Luo C.F.,Liu Q.H.,Shi S.Z.,and Cui J.,2012,The study of RNA editing sites of the atp6 gene transcripts in suger-beet mitochondria,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),10(4):428-432(施真,程大友,罗成飞,刘巧红,史淑芝,崔杰,2012,甜菜线粒体atp6基因转录本的RNA编辑位点研究,分子植物育种,10(4):428-432)
Yang J.H.,Zhang M.F.,and Yu J.Q.,2009,Mitochondrial nad2gene is co-transcripted with CMS-associated orfB gene in cytoplasmic male-sterile stem mustard(Brass ica juncea),Molecular Biology Reports,36(2):345-351
Yao Y.Q.,Zhang G.S.,Liu H.W.,and Wang JM.,2002,Studies on correlation between intine and K-type cytoplasmic male sterility in wheat,Xibei Zhiwu Xuebao(Acta Botanica Boreal-Occidentalia Sinica),22(2):333-337(姚雅琴,张改生,刘宏伟,王军卫,2002,K型小麦花粉粒内壁及ATP酶活性与雄性不育的相关性,西北植物学报,22(2):333-337)
Yi F.Y.,Shi F.L.,Gao C.P.,Xiong M.,Ye RE,and Cai L.Y.,2014,A comparative analysis of physiological and biochemical characteristic between alfalfa male sterile plants and male fertile plants,Zhongguo Caodi Xuebao(Chinese Journal of Grassland),6(6):60-65(伊风艳,石凤翎,高翠萍,熊梅,晔薷罕,蔡丽艳,2014,首蓿雄性不育株与可育株生理生化特性的比较,中国草地学报,36(6):60-65)
Yu S.Z.,Zhang L.,Zhang J.,and Zhao J.H.,2015,The research progress of genomics in Nicotiana Species,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(7):1541-1548(余世洲,张磊,张洁,赵杰宏,2015,烟属物种基因组研究进展,基因组学与应用生物学,34(7):1541-1548)
Zhang J.,Zhu Q.D.,Ju L.,Zhang G.S.,Yu Y.A.,Niu N.,Wang J W.,and Ma S.C.,2015,Organization changes of actin cytoskeleton and callose with the expression analysis of related genes in physiological male sterile wheat,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),48(14):2687-2696(张姣,朱启迪,巨岚,张改生,于永昂,牛娜,王军卫,马守才,2015,小麦生理型雄性不育系微丝骨架和脱肌质的变化与其相关基因的表达分析,中国农业科学,48(14):2687-2696)
Zhang Z.,Liang Y.,Ding H.F.,and Zhang HM.,2012,Study on cloning and expression of atp9 gene in carrot and its relationship to cytoplasmic male sterility,Beifang Yuanyi(Northern Horticulture),(24):140-144(张智,梁毅,丁海凤,张洪伟,2012,胡萝卜atp 9基因的克隆表达及细胞质雄性不育的相关性研究,北方园艺,(24):140-144)
Zhao T.,Zhu T.Y.,Liu Q.Y.,Zhang M.L.,and Jiang H.Y.,2009,SNP in tobacco mitochondrial gene atp6 and its correlation with CMS,Zuowu Xuebao(Acta Agronomica Sinica),35(9):1655-1661赵婷,朱滕义,刘齐元,张美良,蒋海燕,2009,烟草线粒体基因atp 6的SNP及其与CMS的相关性,作物学报,35(9):1655-1661)
Zhou B.,Sha W.,Zhang M.J.,Zhang C.L.,and Suo L.,2015,Cloning and expression analysis of ATP synthaseⅡsubunit gene RcatpG in Racomitrium canescens,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(7):1435-1442(周伯,沙伟,张梅娟,张春蕾,索荔,2015,砂藓ATP合酶Ⅱ亚基基因的克隆及表达分析,基因组学与应用生物学,34(7):1435-1442)
Zhou W.,Liu Q.Y.,Chen X.F.,Liu F.H.,Cao H.,and Liu C.Q.,2007,Studies on atp9 mRNA related to cytoplasmic male sterility in tobacco,Guangxi Zhiwu(Guihaia),27(1):114-120(周玮,刘齐元,陈雪峰,刘飞虎,曹槐,刘次全,2007,与烟草细胞质雄性不育相关的线粒体基因atp9的mRNA研究,广西植物,27(1):114-120)
Zhou W.,Liu Q.Y.,Zhu T.Y.,Fang D.D.,Zhu X.W.,and Cheng Y.Q.,2012,Bioinformatics analysis of atp6 gene related to tobacco male sterility,HeNong XueBao(Journal of Nuclear Agricultural Sciences),26(1):0054-0059(周玮,刘齐元,朱腾义,范东东,朱肖文,程元强,2012,雄性不育烟草atp6基因育性相关生物信息学分析,核农学报,26(1):0054-0059)
Zhu T.Y.,Fan D.D.,Zhao T.,Liu Q.Y.,Wang J.G.,Zhu X.W.,and Cheng Y.Q.,2010,SNP detection in tobacco(Nicotiana tabacum)mitochondrial gene coxⅡand its association with CMS,Henong Xuebao(Journal of Nuclear Agricultural Sciences),24(4):720-727(朱腾义,范东东,赵婷,刘齐元,王建革,朱肖文,程元强,2010,烟草线粒体基因coxⅡ的SNP检测及其与CMS的相关性分析,核农学报,24(4):720-727)
基本信息:
DOI：10.13417/j.gab.035.001464
中图分类号:Q943.2
引用信息:
[1]陶瑶,王瑜,吴凌敏,等.细胞质雄性不育烟草orfB基因的PCR扩增与序列分析[J].基因组学与应用生物学,2016,35(06):1464-1470.DOI:10.13417/j.gab.035.001464.
基金信息:
国家自然科学基金项目(31260350;31301388);; 江西省教育厅科技计划项目(GJJ13275)共同资助
2016-06-25
2016-06-25