| 333 | 0 | 19 |
| 下载次数 | 被引频次 | 阅读次数 |
野油菜黄单胞菌ATCC 33913基因组中Xcc0383、Xcc1678被标注丙二酸单酰-ACP甲基转移酶(BioC)编码基因,从KEGG数据库检索Xcc0383和Xcc1678的氨基酸序列,运用生物信息学在线分析软件对这2个基因编码的蛋白进行了理化性质、蛋白结构、亲水性、跨膜结构、信号肽、磷酸化位点及蛋白相互作用网络的预测分析。结果表明2个蛋白理化性质相近,但Xcc0383为亲水性蛋白,Xcc1678位疏水性蛋白。2个蛋白都具有甲基转移酶功能结构域,不存在跨膜结构域和信号肽,磷酸化位点和翻译后修饰位点的数量和位置类似。Xcc0383和Xcc1678二级结构中组成最多的是α-螺旋,分别占46.36%和51.69%,三级结构预测结果与二级结构一致。蛋白相互作用预测后发现,Xcc0383与BioH、BioF、BioA等负责生物素合成的蛋白关系最为密切,而Xcc1678与其毗邻的糖基转移酶关系密切。氨基酸序列同源比对显示Xcc0383与大肠杆菌(E.coli)的BioC相似性较高。Xcc0383编码蛋白负责在野油菜黄单胞菌体内合成生物素,而Xcc1678作为甲基转移酶负责为不同的受体分子提供甲基基团。
Abstract：Two genes in Xanthomonas campestris ATCC 33913, Xcc0383 and Xcc1678, were annotated as malonyl-ACP methyltransferase. We analyzed and predicted the physicochemical properties, structures, hydrophilicity,transmembrane domain, signal peptide, phosphorylation sites and protein interaction network of Xcc0383 and Xcc1678 by using bioinformatics analysis tool. The results showed that the two proteins had similar physicochemical properties, but Xcc0383 was a hydrophilic protein, with Xcc1678 a hydrophobic protein. Transmembrane domain and signal peptide did not exist in both of them, and the number and location of phosphorylation and post-translational modification sites were similar. The secondary structures of Xcc0383 and Xcc1678, α-helix was the most important component, accounting for 46.36% and 51.69%, respectively, and the tertiary structure prediction results are consistent with the secondary structure. The protein interaction network prediction showed that Xcc0383 was most closely related to the proteins responsible for biotin synthesis, such as BioH, BioF, and BioA,etc. Xcc1678 was closely related to its adjacent glycosyltransferase. Combined with the results of protein sequence alignment, Xcc0383 shared higher sequence identity with the E. coli BioC, compared with Xcc1678. Therefore, it is speculated that the Xcc0383 was responsible for the synthesis of biotin in Xanthomonas campestris, while Xcc1678 acts as a methyltransferase responsible for providing methyl groups for other receptors.
Cronan J.E.,2014,Biotin and lipoic acid:synthesis,attachment,and regulation,EcoSal Plus,doe:10.1128/ecosalplus.3.6.3.5.
Cryle M.J.,and Schlichting I.,2008,Structural insights from a P450 carrier protein complex reveal how specificity is achieved in the P450(Biol)ACP complex,Proc.Natl.Acad.Sci.USA,105(41):15696-15701
Fan S.H.,2014,Functional identification and catalytic mechanism of BioF in biotin synthesis pathway of Mycobacteria,Dissertation for Ph.D.,Shandong University,Supervisors:Zhang X.E.,and Chen G.J.,pp.15-16(范尚华,2014,分枝杆菌生物素合成途径中BioF的功能鉴定和基于结构的催化机制研究,博士学位论文,山东大学,导师:张先恩,陈冠军,pp.15-16)
Feng Y.J.,Napier B.A.,Manandhar M.,Henke S.K.,Weiss D.S.,and Cronan J.E.,2014,A Francisella virulence factor catalyses an essential reaction of biotin synthesis,Mol.Microbiol.,91(2):300-314
Kubota T.,Shimono J.,Kanameda C.,and Izumi Y.,2007,The first thermophilic alpha-oxoamine synthase family enzyme that has activities of 2-amino-3-ketobutyrate CoA ligase and 7-keto-8-aminopelargonic acid synthase:Cloning and overexpression of the gene from an extreme thermophile,Thermus thermophilus,and characterization of its gene product,Bioscience Biotechnology and Biochemistry,71(12):3033-3040
Liu X.,Liu K.W.,and Yu D.,2004,Reviews on biotin,Sichuang Shipin yu Fajiao(Sichuan Food and Fermentation),(2):15-16(刘鑫,刘克武,喻东,2004,生物素的研究进展,四川食品与发酵,(2):15-16
Napier B.A.,Meyer L.,Bina J.E.,Miller M.A.,Sjostedt A.,and Weiss D.S.,2012,Link between intraphagosomal biotin and rapid phagosomal escape in Francisella,Proc.Natl.Acad.Sci.USA,109(44):18084-18089
Ren X.M.,Wang X.L.,Shi H.Y.,Zhang X.M.,Chen Z.C.,Malhi K.K.,Ding C.,and Yu S.Q.,2018 Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis,bacterial morphology and virulence,Scientific Reports,8(1):1198
Shapiro M.M.,Chakravartty V.,and Cronan J.E.,2012,Remarkable diversity in the enzymes catalyzing the last step in synthesis of the pimelate moiety of biotin,PLoS ONE,7(11):e49440
Singh S.,Dey S.S.,Bhatia R.,Batley J.,and Kumar R.,2018,Molecular breeding for resistance to black rot Xanthomonas campestris pv.campestris(Pammel)Dowson in Brassicas:recent advances,Euphytica,214(10):2-17
Vicente J.G.,and Holub E.B.,2013,Xanthomonas campestris pv.campestris(cause of black rot of crucifers)in the genomic era is still a worldwide threat to brassica crops,Molecular Plant Pathology 14(1):2-18
Wu J.H.,Zhang Y.Q.,and Tang Y.,2016,Application of data mining in bioinformatics-bibliometrics perspective,Shengwu Xinxixue(Chinese Journal of Bioinformatics),14(4):249-253(吴金华,张艳秋,唐毅,2016,数据挖掘在生物信息学中的应用--文献计量学视角,生物信息学,14(4):249-253
Yang Z.J.,and Qian J.,2018,Bioinformatics analysis of glucokinase gene of Xanthomonas campestris pv.campestris,Kaili Xueyuan Xuebao(Journal of Kaili University),36(3):70-73(杨正久,钱静,2018,野油菜黄单胞菌葡萄糖激酶的生物信息学分析,凯里学院学报,36(3):70-73
Yu J.,Niu C.,Wang D.C.,Li M.,Teo W.S.,Sun G.,Wang J.P.,Liu J.,and Gao Q.A.,2011,MMAR_2770,a new enzyme involved in biotin biosynthesis,is essential for the growth of Mycobacterium marinum in macrophages and zebrafish,Microbes and Infection,13(1):33-41
Yuan Q.L.,Fu Y.R.,and Yi Z.J.,2018,Bioinformatic analysis of the LepB protein from Mycobacterium tuberculosis and prediction of its function,Zhongguo Bingyuan Shengwuxue Zazhi(Journal of Pathogen Biology),13(8):814-818(袁秋露,付玉荣,伊正君,2018,结核分枝杆菌LepB蛋白的功能预测及生物信息学分析,中国病原生物学杂志,13(8):814-818)
Zlitni S.,Ferruccio L.F.,and Brown E.D.,2013,Metabolic suppression identifies new antibacterial inhibitors under nutrient limitation,Nature Chemical Biology,9(12):796-804
基本信息:
DOI：10.13417/j.gab.039.005091
中图分类号:S432.4
引用信息:
[1]陈群一,吴晓妍,陈博,等.野油菜黄单胞菌BioC蛋白的生物信息学分析[J].基因组学与应用生物学,2020,39(11):5091-5099.DOI:10.13417/j.gab.039.005091.
基金信息:
国家自然科学基金项目(No.31601601; 31671987)资助
2020-11-25
2020-11-25