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从南海海绵Dysidea avara中分离筛选得到一株产弱碱性脲酶菌株Bacillus atrophaeus C89,为探究其在海洋环境重金属离子检测中的应用,在人工海水体系中研究Hg2+、Cu2+、Pb2+和Cd2+四种重金属离子对脲酶活性的影响。研究发现四种重金属离子的浓度与脲酶抑制率呈显著的正相关性,当仅有单一重金属离子存在时,各重金属离子对脲酶活性的抑制效应顺次为Hg2+>Cu2+>Cd2+>Pb2+;当有多种重金属离子同时存在时,联合抑制效应与抑制率较高的重金属离子接近,且不同组合存在不同的协同或拮抗效应,Hg2+、Cu2+、Cd2+呈现协同效应,而Pb2+表现出一定的拮抗效应。与刀豆脲酶对比发现,海洋微生物脲酶在p H和盐度等方面具有更高的耐受性,更适用于海洋环境重金属离子的检测。
Abstract：Bacillus atrophaeus C89,isolated from the South China Sea sponge Dysidea avara,is able to produce weak-alkaligenous urease.In order to explore its application in the detection of heavy metal ion in marine environments,the effects of Hg2+,Cu2+,Pb2+and Cd2+on the activity of urease in artificial seawater system were investigated.It was found that the four heavy metal ion concentration was positively related to urease rate.If the artificial seawater system contained a sole heavy metal ion,the inhibition rate was:Hg2+>Cu2+>Cd2+>Pb2+.In the case of various heavy metal ion in the artificial sea water,the combined inhibitory effect was close to the heavy metal ion with high inhibition rate,and there were different synergistic or antagonisticinteractions between different combinations.Hg2+,Cu2+and Cd2+presented synergistic effect,while Pb2+presented antagonistic effect.By the comparison with Jack bean urease,the marine microbial urease showed higher tolerance than Jack bean urease in p H and salinity.It is more suitable for the detection of heavy metal ions in the marine environment.
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基本信息:
DOI：10.13417/j.gab.037.003407
中图分类号:Q936;X55
引用信息:
[1]金结平,张风丽.重金属离子对海洋微生物脲酶活性影响的研究[J].基因组学与应用生物学,2018,37(08):3407-3413.DOI:10.13417/j.gab.037.003407.
2018-08-24
2018-08-24