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近年来随着环境与气候的变化，人和动物体不断出现新的病原微生物，对人和动物的健康与生产造成重大影响。常规病原分离鉴定技术难于及早发现病原，加之对其致病性认识的不足，常导致不能有效预防和控制。二代测序技术的发展与测序效率的提高，使得微生物宏基因组学研究成为新的研究热点之一。基于二代测序技术的宏基因组学能够通过大量的基因测序、序列拼接实现群体微生物的鉴定，为疾病诊断和病原微生物的鉴定提供了可靠依据。本文就宏基因组学技术在动物传染病原鉴定、耐药性及遗传变异方面的研究进行综述。
Abstract：Novel pathogenic microorganisms continuously appeared with changes in the environment and climate in recent years, which impact on health of humans and animals. Conventional isolation and identification for pathogens were difficult to find them at early stage. Furthermore, these infectious diseases could not be prevented and controlled effectively for insufficient knowledge to characters and pathogenicity of pathogens. With the improvement of next-generation sequencing technology in efficiency, microbial metagenomics research was becoming one of hotspots in etiology and clinical epidemiology. Metagenomics based on next-generation sequencing would identify target or unknown microorganisms through a high-throughput gene sequencing and assembling to microbiota. These provided a reliable basis for pathogens identification and clinical diagnosis. The procedures and methods of metagenomics on pathogens identification, antibiotic resistance and genetic variation were reviewed in this article.
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基本信息:
DOI：10.13417/j.gab.040.003475
中图分类号:S852.6
引用信息:
[1]敬晓棋,李娟,郝婷婷,等.宏基因组学技术在动物传染病原挖掘中的应用与进展[J].基因组学与应用生物学,2021,40(Z4):3475-3480.DOI:10.13417/j.gab.040.003475.
基金信息:
国家自然科学基金项目（31960710; 32060810）; 榆林市产学研项目（CXY-2020-011）; 榆林学院高学历人才启动金（14GK38）共同资助
2021-05-28
2021-05-28
2021-05-28