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白刺花种子是高活力硬实种子,其种皮厚且坚韧,限制水分的渗入和种胚内气体交换,从而抑制种子的呼吸作用,并对种胚的发育存在机械束缚,若不经处理,发芽率极低。实验采用不同浓度(0 mg/L,50 mg/L,100 mg/L,200 mg/L,300 mg/L,500 mg/L)的赤霉素(GA)对白刺花种子进行不同时间的处理,测定种子质量、长、宽、厚、种皮厚度、吸水位点、萌发率、根长等生理生化指标,以探讨GA对白刺花种子萌发及根生长状况的影响。结果显示白刺花种子种皮较厚且坚硬,吸水位点不只在种脐,在打磨条件下用200 mg/L的GA3处理种子萌发率最高,达到95%,根生长量最大,达到0.05 cm。
Abstract：Seed of Sophora davidii is hard and of high vigor, of which the seed coat is thick and tough, limiting the penetration of water and exchange of gas in embryo, thereby inhibiting respiration of seeds and mechanically restraining the growth of embryo. If not treated, the germination rate would be very low. In this study, the Sophora davidii seeds were treated by gibberellin(GA) with different concentrations(0 mg/L, 50 mg/L, 100 mg/L, 200 mg/L,300 m/L, 500 mg/L) for different times. Then, we measured the length, width, thickness, testa thickness, water absorption sites, germination rate, root length and other physiological and biochemical indexes of seeds, so as to discuss the effect of GA on seed germination and root formation condition of Sophora davidii. The result showed that seed of Sophora viciifolia had thick and hard coat, and its water absorption sites were not only on hilum. After polishing, seeds treated with 200 mg/L GA3 had the highest germination rate, which reached 95%. Also, its root growth was the longest, reaching 0.05 cm.
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基本信息:
DOI：10.13417/j.gab.036.003119
中图分类号:Q945.34;S793.9
引用信息:
[1]刘杰,向锐,罗充,等.GA对白刺花种子萌发的影响[J].基因组学与应用生物学,2017,36(08):3119-3124.DOI:10.13417/j.gab.036.003119.
基金信息:
贵州省科技计划项目(黔科合LH字(2016)7210号);; 贵州省植物生理与发育调控重点实验室建设;; 贵州省重点实验室建设项目(黔科合计Z字(2011)4005);; 教育部喀斯特山地生物多样性保护与可持续利用创新团队;; 长江学者和创新团队发展计划项目(IRT1227);; 贵州师范大学博士科研启动项目(11904-0514012)共同资助
2017-03-13
2017-03-13
2017-03-13