| 395 | 18 | 51 |
| 下载次数 | 被引频次 | 阅读次数 |
乙烯是重要的植物激素,在植物生长发育和胁迫响应中具有重要的调节功能。1-氨基环丙烷-1-羧酸氧化酶(ACO)是植物乙烯生物合成途径中的一个关键酶。根据转录组数据,从菠萝中成功克隆到1个编码ACO的基因Ac ACO1,测序结果证实该基因全长1 094 bp,包含一个957 bp的开放阅读框,编码318个氨基酸。AcACO1蛋白具有典型的植物ACO结构特征,含有DIOXN和2OG-FeⅡ_Oxy两个保守结构域。1 850 bp的AcACO1启动子区域含有众多应答激素和环境胁迫信号元件。实时荧光定量分析结果表明,乙烯利、低温和赤霉素均能诱导AcACO1的表达。
Abstract：Ethylene is an important hormone regulating the growth, development and stress response of plants.1-amino cyclopropane-1-carboxylic acid oxidase(ACO) is a decisive enzyme in plant ethylene biosynthesis. In this study, a gene named AcACO1 in Ananas comosus coding ACC oxidase was cloned accoding to transcriptome data. The full-length c DNA of AcACO1 was 1 094 bp, containing a 957 bp ORF, which encoded 318 amino acids.AcACO1 protein had two typical conserved motifs DIOXN and 2 OG-Fe Ⅱ_Oxy in plant ACC oxidase. Several regulatory elements related to hormone and stress responses were found in 1 850 bp putative promoter region of AcACO1. The Real-time RT-PCR results indicated that the expression of AcACO1 was significantly induced by ethylene, low temperature and gibberellin(GA3).
Bhugaloo R.A.,2003,Pineapple:effect of different flower induction times on crop cycle length and yield,Food and Agricultural Research Council,23:83-90
Botella J.R.,Cavallaro A.S.,Cazzonelli C.I.,Subhadrabandhu S.,and Chairidchai P.,2000,Towards the production of transgenic pineapple to control flowering and ripening,Acta Horticulturae,529(529):115-122
Cazzonelli C.I.,Cavallaro A.S.,and Botella J.R.,1998,Cloning and characterisation of ripening-induced ethylene biosynthetic genes from non-climacteric pineapple(Ananas comosus)fruits,Functional Plant Biology,25(5):513-518
Cunha and Da G.A.P.,2005,Applied aspects of pineapple flowering,Bragantia,64(4):499-516
Jafari Z.,Haddad R.,Hosseini R.,and Garoosi G.,2012,Cloning,identification and expression analysis of ACC oxidase gene involved in ethylene production pathway,Mol.Biol.Rep.,40(2):1341-1350
Li Z.Y.,Yi Z.L.,Cong H.Q.,and Xu L.,2012,Regulation of Ca2+regulators on the Ca M gene expression during ethylene-induced flowering in Guzmania“Amaranth”,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),31(1):35-39(李志英,易籽林,丛汉卿,徐立,2012,Ca2+调节剂在乙烯催花中对观赏凤梨Ca M基因表达特性的调节作用,基因组学与应用生物学,31(1):35-39)
Light K.M.,Wisniewski J.A.,Vinyard W.A.,and Kieberemmons M.T.,2016,Perception of the plant hormone ethylene:known-knowns and known-unknowns,J.Biol.Inorg.Chem.,21(5-6):1-14
Lin Z.,Zhong S.,and Grierson D.,2009,Recent advances in ethylene research,J.Exp..Bot.,60(12):3311-3336
Liu C.Y.,Rui-Hua L.,Jun L.I.,Zhao A.C.,Wang X.L.,Diane U.,Wang X.H.,Wang C.H.,Yu Y.S.,Han S.M.,Lu C.,and Yu M.D.,2014,Characterization and expression profiles of Ma ACS and Ma ACO genes from mulberry(Morus alba L.),Journal of Zhejiang University(Science B),15(7):611-623
Liu Y.,Liu C.H.,Fan C.,Wu J.F.,Dai H.F.,and Chen J.R.,2012,Influence of exogenous GA3 application on growth and chemical quality of pineapple fruits,Guangdong Nongye Kexue(Guangdong Agricultural Sciences),39(12):42-43(刘岩,刘传和,凡超,吴洁芳,戴宏芬,陈锦瑞,2012,赤霉素对菠萝果实生长发育及品质的影响,广东农业科学,39(12):42-43)
Nagtong T.,Thanonkeo S.,Klanrit P.,and Thanonkeo P.,2009,Cloning and characterization of 1-aminocyclopropane-1-carboxylate oxidase gene from orchid(Dendrobium spp.),World Applied Sciences Journal,7(1):11-18
Pan G.,and Lou C.F.,2008,Isolation of an 1-aminocyclopropane-1-carboxylate oxidase gene from mulberry(Morus alba L.)and analysis of the function of this gene in plant development and stresses response,J.Plant Physiol.,165(11):1204-1213
Shi H.Y.,and Zhang Y.X.,2012,Cloning,expression and regulation of ACC oxidase gene in higher plants,Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),40(1):39-40(石海燕,张玉星,2012,高等植物ACC氧化酶基因的克隆表达及其调控,安徽农业科学,40(1):39-40)
Shi Q.Y.,Wang S.,and Zhang R.L.,2013,Impact of extraneous ethylene,naphthylacetic acid and gibberellin to flowering of three kinds of air plant of Tillandsia,Jiangsu Linye Keji(Journal of Jiangsu Forestry Science and Technology),40(2):11-13(史清云,王姗,张荣良,2013,外源乙烯利、萘乙酸和赤霉素对3种空气凤梨开花性状的影响,江苏林业科技,40(2):11-13)
Tang X.,Gomes A.,Bhatia A.,and Woodson W.R.,1994,Pistil-specific and ethylene-regulated expression of 1-aminocyclopropane-1-carboxylate oxidase genes in petunia flowers,Plant Cell,6(9):1227-1239
Trusov Y.,and Botella J.R.,2006,Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple(Ananas comosus(L.)Merr.),J.Exp.Bot.,140(9):1714-1720
Wang F.,Cui X.,Sun Y.,and Dong C.H.,2013,Ethylene signaling and regulation in plant growth and stress responses,Plant Cell Reports,32(7):1099-1109
Wu J.Y.,Li C.Q.,Lu W.J.,and Li J.G.,2013,Cloning of Lc-A-CO1 and its expression related to fruitlet abscission in litchi,Guoshu Xuebao(Journal of Fruit Science),30(2):207-213(吴建阳,李彩琴,陆旺金,李建国,2013,荔枝ACO1基因克隆及其与幼果落果的关系,果树学报,30(2):207-213)
Xue L.J.,Zhou J.H.,and Xing H.C.,2013,Cloning and charac terization of ACC oxidase gene(Bn ACO1)from rame(Boehmeria nivea),Zhongguo Nongye Kexue(Scientia Agricultura Scienca),46(11):2377-2385(薛丽君,周精华,邢虎成,2013,苎麻ACC氧化酶基因(Bn ACO1)的克隆及表达研究,中国农业科学,46(11):2377-2385)
Xun C.G.,Hao A.M.,Zhang R.M.,and Yang P.,2005,Research of flowering time regulation technology in pineapple flowers,Anhui Nongxue Tongbao(Anhui Agricultural Science Bulletin),11(6):102-104(徐晨光,郜爱民,张瑞明,严平,2005,凤梨科花卉花期调控技术研究,安徽农学通报,11(6):102-104)
Zhang Z.L.,Fan H.Y.,Hua M.,and He F.,2012,Induction of pineapple flowering and underlying physiological and molecular bases,Redai Zuowu Xuebao(Chinese Journal of Tropical Crops),33(5):950-995(张治礼,范鸿雁,华敏,何凡,2012,菠萝开花诱导及其生理与分子基础,热带作物学报,33(5):950-955)
Zhu Z.Y.,Yang Y.M.,Sun G.M.,and Liu S.H.,2012,Natural flowering in pineapple and its inhibition technique,Zhongguo Reidai Nongye(China Tropical Agriculture),(5):75-77(朱祝英,杨玉梅,孙光明,刘胜辉,2012,菠萝自然开花及其抑制技术,中国热带农业,(5):75-77)
基本信息:
DOI：10.13417/j.gab.037.000339
中图分类号:Q943.2;S668.3
引用信息:
[1]朱家红,李超燕,范鸿雁,等.菠萝ACC氧化酶基因AcACO1的克隆与表达分析[J].基因组学与应用生物学,2018,37(01):339-344.DOI:10.13417/j.gab.037.000339.
基金信息:
国家自然科学基金项目(31260460)资助
2018-01-25
2018-01-25