| 72 | 0 | 55 |
| 下载次数 | 被引频次 | 阅读次数 |
丝裂原活化蛋白激酶（mitogen-activated protein kinase,MAPK/MPK）级联反应是常见且保守的信号转导通路，在植物中发挥着重要作用，涉及多种生物和非生物胁迫反应以及生长发育过程。目前对于MAPK基因家族的鉴定与分析已有较为系统的研究，但在甘蓝型油菜（Brassica napus）中，对该基因家族成员的鉴定和分析仍不够全面。本研究以拟南芥（Arabidopsis thaliana）基因为参考，采用生物信息学手段开展研究。从芸薹属（Brassica）的三个四倍体种[埃塞俄比亚芥（B. carinata），芥菜型油菜（B. juncea）和甘蓝型油菜（B. napus）]和三个二倍体种[黑芥（B. nigra），甘蓝（B. oleracea）和白菜（B. rapa）]中，共鉴定出291个MAPK基因家族成员。对甘蓝型油菜及其二倍体祖先的MAPK基因进行了系统进化分析、蛋白质理化性质及亚细胞定位预测分析、蛋白保守基序组成、蛋白保守结构域与基因结构分析、基因的染色体定位、共线性与Ka/Ks分析以及顺式作用元件分析和转录组分析。研究结果显示：MAPK基因可划分为4个亚组。在甘蓝型油菜的进化历程中，发生了内含子/外显子的增减事件。同时，MAPK基因在进化过程中主要受到强烈的纯化选择作用的调控。此外，在其启动子区域中，发现了多种顺式调控元件，涉及光照反应、脱落酸信号传导及低氧胁迫等生物学过程。转录组数据也揭示这些基因在不同组织中呈现出特异性的表达模式，并对多种处理条件展现出差异化的响应趋势。本研究系统鉴定并解析了芸薹属多物种的MAPK基因家族的特征，为阐明该基因家族在甘蓝型油菜生长发育及逆境响应中的分子机制提供了重要的理论依据。
Abstract：The mitogen-activated protein kinase(MAPK~/MPK) cascade is a common and conserved signaling pathway that plays a crucial role in plants, involved in various responses to both biotic and abiotic stresses, as well as in growth and development processes.While systematic studies on the identification and analysis of theMAPKgene family have been conducted, comprehensive analysis of the family members inBrassica napusremains insufficient. In this study, we usedArabidopsis thalianaas a reference and employed bioinformatics approaches for research. A total of 291 MAPKgene family members were identified from three allotetraploid(Brassica carinata, Brassica juncea, andBrassica napus) and three diploid(Brassica nigra, Brassica oleracea,andBrassica rapa) species of the Brassicagenus. Systematic phylogenetic analysis, physicochemical properties and subcellular localization predictions, motif composition, conserved domain and gene structure analysis, chromosomal localization, collinearity andKa/Ksanalysis, as well ascis-element analysis and transcriptome analysis were performed on theMAPKgenes ofBrassica napusand its diploid ancestors. The results showed that theMAPKgenes could be classified into four groups. During the evolutionary process ofBrassica napus, intron~/exon gain and loss events occurred. Furthermore, theMAPKgenes were primarily regulated by strong purifying selection throughout their evolution. In addition, severalcis-regulatory elements related to light response, abscisic acid signaling, and hypoxic stress were found in their promoter regions. Transcriptome data further revealed that these genes exhibited tissue-specific expression patterns and showed differential responses to various treatment conditions. In this study, theMAPKgene family was systematically identified and analyzed across multiple Brassicaspecies. This work provides a crucial theoretical basis for elucidating the molecular mechanisms underlying the roles of this gene family in the growth, development, and stress responses ofBrassica napus.
BLUM M,ANDREEVA A,FLORENTINO L C,et al.,2025.InterPro:the protein sequence classification resource in 2025.Nucleic Acids Res.,53(d1):D444-D456.
CHEN C J,WU Y,LI J W,et al.,2023.TBtools-Ⅱ:a “one for all,all for one” bioinformatics platform for biological big-data mining.Mol.Plant,16(11):1733-1742.
CHEN Y H,WANG N N,ZHANG J B,et al.,2020.Genome-wide identification of the mitogen-activated protein kinase (MAPK) family in cotton (Gossypium hirsutum) reveals GhMPK6 involved in fiber elongation.Plant Mol.Biol.,103(4):391-407.
CHENG F,MANDÁKOVÁ T,WU J,et al.,2013.Deciphering the diploid ancestral genome of the mesohexaploid Brassica rapa.Plant Cell,25(5):1541-1554.
DE ZELICOURT A,COLCOMBET J,HIRT H,2016.The role of MAPK modules and ABA during abiotic stress signaling.Trends Plant Sci.,21(8):677-685.
FAN Z Y,ZHAO B B,ZENG Y H,et al.,2024.MaMPK19,a key gene enhancing cold resistance by activating the CBF pathway in banana.Plant Physiol.Biochem.,217:109290.
JONAK C,ÖKRÉSZ L,BÖGRE L,et al.,2002.Complexity,cross talk and integration of plant MAP kinase signalling.Curr.Opin.Plant Biol.,5(5):415-424.
JOO S,LIU Y,LUETH A,et al.,2008.MAPK phosphorylation-induced stabilization of ACS6 protein is mediated by the non-catalytic C-terminal domain,which also contains the cis-determinant for rapid degradation by the 26S proteasome pathway.Plant J.,54(1):129-140.
KATOH K,STANDLEY D M,2013.MAFFT multiple sequence alignment software version 7:improvements in performance and usability.Mol.Biol.Evol.,30(4):772-780.
NEUPANE A,NEPAL M P,PIYA S,et al.,2013.Identification,nomenclature,and evolutionary relationships of mitogen-activated protein kinase (MAPK) genes in soybean.Evol.Bioinform.Online,9:363-386.
NIU Y,LIU Q Q,HE Z S,et al.,2024.A Brassica carinata pan-genome platform for Brassica crop improvement.Plant Commun.,5(1):100725.
PIAO Y L,JIN K N,HE Y,et al.,2018.Genome-wide identification and role of MKK and MPK gene families in clubroot resistance of Brassica rapa.PLoS ONE,13(2):e0191015.
RASMUSSEN M W,ROUX M,PETERSEN M,et al.,2012.MAP kinase cascades in Arabidopsis innate immunity.Front.Plant Sci.,3:169.
REYNA N S,YANG Y,2006.Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection.Mol.Plant Microbe Interact.,19(5):530-540.
RODRIGUEZ M C,PETERSEN M,MUNDY J,2010.Mitogen-activated protein kinase signaling in plants.Annu.Rev.Plant Biol.,61:621-649.
VISION T J,BROWN D G,TANKSLEY S D,2000.The origins of genomic duplications in Arabidopsis.Science,290(5499):2114-2117.
WANG G,WANG T,JIA Z H,et al.,2018.Genome-wide bioinformatics analysis of MAPK gene family in kiwifruit (Actinidia chinensis).Int.J.Mol.Sci.,19(9):E2510.
WANG Y,TANG H,DEBARRY J D,et al.,2012.MCScanX:a toolkit for detection and evolutionary analysis of gene synteny and collinearity.Nucleic Acids Res.,40(7):e49.
WU P,WANG W L,LI Y,et al.,2017.Divergent evolutionary patterns of the MAPK cascade genes in Brassica rapa and plant phylogenetics.Hortic.Res.,4:17079.
ZHANG M M,SU J B,ZHANG Y,et al.,2018.Conveying endogenous and exogenous signals:MAPK cascades in plant growth and defense.Curr.Opin.Plant Biol.,45:1-10.
ZHANG S Q,KLESSIG D F,2001.MAPK cascades in plant defense signaling.Trends Plant Sci.,6(11):520-527.
ZHAO C Z,WANG P C,SI T,et al.,2017.MAP kinase cascades regulate the cold response by modulating ICE1 protein stability.Dev.Cell,43(5):618-629.e5.
基本信息:
DOI：10.13417/j.gab.044.001230
中图分类号:Q943.2;S565.4
引用信息:
[1]刘云彬,叶思莹,冯紫琰,等.甘蓝型油菜MAPK基因家族的全基因组鉴定及表达分析[J].基因组学与应用生物学,2025,44(12):1230-1243.DOI:10.13417/j.gab.044.001230.
基金信息:
四川省科技计划项目（2025ZNFSC1006）; 国家自然科学基金项目（32100526）; 重庆市技术创新与应用发展重大专项（CSTB2024TIADKPX0025）共同资助