| 392 | 14 | 143 |
| 下载次数 | 被引频次 | 阅读次数 |
为了解团水虱不同生长阶段的肠道微生物多样性,采用Mi Seq高通量测序技术和生物信息学分析手段,构建团水虱幼体、稚体和成体三个生长阶段肠道微生物的16S r RNA基因测序文库,分析三个样品中的菌群组成和生物多样性。结果表明,成体(T1)、稚体(T2)、幼体(T3)三个生长阶段肠道微生物样品共产生794个OTU,分别含646个、686个和156个OTU。分析显示,共检测到25个细菌门类,其中包括变形菌门、拟杆菌门、梭杆菌门等3个优势菌门。变形菌门为三个样品的共同优势菌,在幼体、稚体和成体中分别占51.7%、55.1%和51.6%;拟杆菌门为稚体(30.21%)和成体(38.57%)的次优势菌门,在幼体肠道内低于5%;梭杆菌门在幼体体内占37.8%,是幼体的次优势菌门,但在稚体和成体体内均低于2%。幼体与其他两个生长阶段的肠道细菌群落结构差异显著,与稚体和成体的共有OTU数量,占检测到的OTU总数的比例均低于25%。其中稚体肠道细菌有着最高丰富度及多样性,其Chao指数为697.15、Shannon指数为4.69,最低的Simpson优势度指数为0.035 1,而幼体肠道细菌丰富度和多样性最低。研究结果表明,团水虱肠道菌群种类丰富,幼体肠道菌群种类及其多样性与稚体、成体差异明显;菌群变化与各生长阶段团水虱生活习性关系密切。
Abstract：To understand the the intestinal microbial diversity of Sphaeroma at different growth stages, we constructed intestinal microbial 16 S r RNA gene sequencing library of three growth stages of Sphaeromathe(larvae),juvenile body and adult and analyzed composition of strains and biodiversity of tree samples using Mi Seq high-throughput sequencing technology and bioinformatics analysis tool. The results showed that intestinal microbial samples of three growth stages(adult(T1), juvenile body(T2), larvae(T3)) totally produced 794 operational taxonomic units(OTU), including 646 OTU, 686 OTU, 156 OTU, respectively. The analysis showed that the 25 categories bacteria were detected, including Proteobacteria, Bacteroidetes and Fusobacteria three predominant phylum.Proteobacteria was the common advantage bacterium of these three samples, which respectively accounted for51.7%, 55.1% and 51.6% in larvae, juvenile and adult. Bacteroidetes was the second advantage bacterium in juvenile and the adults, accounting for 30.21% and 38.57% respectively while accounting for less than 5% in the larva; Fusobacteria was the second advantage bacterium in the larva, accounting for 37.8%, but it accounted for less than 2% in both juvenile and the adult. The larvae intestinal bacterial community structure had a significant difference from other two growth stages, but the OTU number them all had was less than 25% in the total number.The juvenile intestinal bacteria had the highest juvenile abundance and diversity, which chao index was 697.15,shannon index was 4.67 and lowest simpson index was 0.035 1, but the larvae body had the lowest richness and diversity. The results of this study showed that intestinal microflora of Sphearoma had abundant species, and the larvae body was significant difference from the juvenile and adult body in intestinal flora species and diversity. So it could speculated that bacterial flora changes were closely related to life habits of Sphearoma at each growth stage.
Abdallah Ismail N.,Ragab S.H.,Abd Elbaky A.,Shoeib A.R.S.Alhosary Y.,and Fekry D.,2011,Frequency of firmicute and bacteroidetes in gut microbiota in obese and norma weight egyptian children and adults,Archives of Medica Science Ams.,7(3):501-507
Benson L.K.,Rice S.A.,and Johnson B.R.,1999,Evidence o cellulose digestion in the wood boring isopod Sphaerom terebrans,Florida Scientist,62(2):128-144
Bergmann G.T.,Bates S.T.,Eilers K.G.,Lauber C.L.,Caporaso J.G.,Walters W.A.,Knight R.,and Fierer N.,2011,The un der-recognized dominance of Verrucomicrobia in soil bac terial communities,Soil Biology and Biochemistry,43(7)1450-1455
Broderick N.A.,Raffa K.F.,Goodman R.M.,and Handelsman J.2004,Census of the bacterial community of the gypsy moth larval midgut by using culturing and culture-independen methods,Appl.Environ.Microbiol.,70(1):293-300
Brooks R.A.,2004,Discovery of Sphaeroma terebrans,a wood boring isopod,in the red mangrove,Rhizophora mangle habitat of northern Florida bay,Ambio.,33(3):171-173
Cheng S.S.,Chang H.T.,Wu C.L.,and Chang S.T.,2007,An ti-termitic activities of essential oils from coniferous trees a gainst Coptotermes formosanus,Bioresour.Technol.,98(2)456-459
Davidson T.M.,Hewitt C.L.,and Campbell M.,2008,Distribu tion,density,and habitat use among native and introduced populations of the Australasian burrowing isopod Sphaero ma quoianum,Biol.Invasions,10(4):399-410
Du S.,Zhang W.F.,and Shi H.T.,2013,16S r RNA-base analysi of bacteroidetes and firmicutes diversity in the microbial flo ra of the red-eared slider(Trachemys scripta elegans)intesti nal tract,Jiyinzuxue Yu Yingyong Shengwuxue(Genomic and Applied Biology),32(6):700-706(杜爽,张文飞,史海涛,2013,基于16S r RNA序列分析红耳龟肠道拟杆菌和厚壁杆菌菌群多样性,基因组学与应用生物学,32(6)700-706)
Egert M.,Stingl U.,Bruun L.D.,Pommerenke B.,Brune A.,and Friedrich M.W.,2005,Structure and topology of microbia communities in the major gut compartments of Melolonth melolontha larvae(Coleoptera:Scarabaeidae),Applied and Environmental Microbiology,71(8):4556-4566
Engelbektson A.,Kunin V.,Wrighton K.C.,Zvenigorodsky NChen F.,Ochman H.,and Hugenholtz P.,2010,Experimenta factors affecting PCR-based estimates of microbial specie richness and evenness,The ISME Journal,4(5):642-647
Flint H.J.,Bayer E.A.,Rincon M.T.,Lamed R.,and White B.A.2008,Polysaccharide utilization by gut bacteria:potentia for new insights from genomic analysis,Nature Review Microbiology,6(2):121-131
Gao F.,Li F.H.,Tan J.,Yan J.P.,and Sun H.L.,2014,Bacteria community composition in the gut content and ambient sedi ment of sea cucumber Apostichopus japonicus revealed b16S r RNA gene pyrosequencing,PLo S One,9(6):e100092
Gao Q.X.,Wu T.X.,and Wang J.B.,2010,Advance in research on symbiotic relationship between intestinal bacterial and their host,Dongwu Yingyang Xuebao(Chinese Journal o Animal Nutrition),22(3):519-526(高权新,吴天星,王进波,2010,肠道微生物与寄主的共生关系研究进展,动物营养学报,22(3):519-526)
Hongoh Y.,Deevong P.,Inoue T.,Moriya S.,Trakulnaleamsai S.Ohkuma M.,Vongkaluang C.,Noparatnaraporn N.,and Ku do T.,2005,Intra-and interspecific comparisons of bacteria diversity and community structure support coevolution o gut microbiota and termite host,Applied and Environmenta Microbiology,71(11):6590-6599
La Rosa T.,Mirto S.,Marino A.,Alonzo V.,Maugeri T.L.,and Mazzola A.,2001,Heterotrphic bacteria community and pollution indicators of mussel-farm impact in the gulf o gaeta(Tyrrhenian Sea),Marine Environmental Research,52(4):301-321
Li C.Y.,Xu Y.J.,Liu X.Z.,Yang H.J.,Shi B.,Shi X.W.,and Zhu X.W.,2015,Comparative analysis of composition,diversity and origin of intestinal bacterial community in pond-and in door tank-culture Japanese flounder(Paralichthys olivaceus)Shuichan Xuebao(Journal of Fisheries of China),39(2)245-255(李存玉,徐永江,柳学周,杨洪军,史宝,史学营,朱学武,2015,池塘和工厂化养殖牙鲆肠道菌群结构的比较分析,水产学报,39(2):245-255)
Li K.,Guan W.,Wei G.,Liu B.,Xu J.,Zhao L.,and Zhang Y.2007,Phylogenetic analysis of intestinal bacteria in the Chi nese mitten crab(Ercheioir sinensis),Journal of Applied Mi crobiology,103(3):675-682
Lin H.W.,and Lin W.H.,2013,The damages and controlling strategies of Sphaeroma in Dongzhaigang mangroves,Reda Linye(Tropical Forestry),41(4):35-36,13(林华文,林卫海,2013,团水虱对东寨港红树林的危害及防治对策,热带林业,41(4):35-36,13)
Liu C.,Li J.B.,Rui J.P.,An J.X.,and Li X.Z.,2015,The applica tions of the 16S r RNA gene in microbial ecology:curren situation and problems,Shengtai Xuebao(Acta Ecologica Sinica),35(9):2769-2788(刘驰,李家宝,芮俊鹏,安家兴李香真,2015,16S r RNA基因在微生物生态学中的应用生态学报,35(9):2769-2788)
Logares R.,Haverkamp T.H.,Kumar S.,Lanzén A.,Nederbragt A.JQuince C.,and Kauserud H.,2012,Environmental microbiol ogy through the lens of highthroughput DNA sequencing:syn opsis of current platforms and bioinformatics approaches Journal of Microbiological Methods,91(1):106-113
Moran D.,Turner S.J.,and Clements K.D.,2005,Ontogeneti development of the gastrointestinal microbiota in the marin herbivorous fish Kyphosus sydneyan us,Microbial Ecology49(4):590-597
Ohkuma M.,Noda S.,Hongoh Y.,and Kudo T.,2002,Divers bacteria related to the bacteroides subgroup of the CFB phy lum within the gut symbiotic communities of various ter mites,Biosci.Biotechnol.Biochem,66(1):78-84
Oikonomou G.,Teixeira A.G.,Foditsch C.,Bicalho M.L.Machado V.S.,and Bicalho R.C.,2013,Fecal microbial di versity in pre-weaned dairy calves as described by pyrose quencing of metagenomic 16S r DNA,associations of faecal ibacterium species with health and growth,Plo S One,8(4)e63157
Prato E.,Danieli A.,Maffia M.,and Biandolino F.,2012,Lipid contents and fatty acid compositions of Idotea baltica and Sphaeroma serratum(Crustacea:Isopoda)as indicators of food sources,Zoological Studies,51(1):38-50
Qiu Y.,2013,Study on digestive enzyme andpopulation ecology of Splraeroma retrolaevis,Thesis for M.S.,Hainan Universi ty,Supervisor:Huang B.,pp.1,18-22(邱勇,2013,光背团水虱消化酶及其种群生态学研究,硕士学位论文,海南大学,导师:黄勃,pp.1,18-25)
Shi J.,Wang Y.,Xu H.R.,Yan G.Z.,Liang H.Z.,and Zhang Q.S.2003,Performance of Lymantria dispar L.'s growth and d velopment on different food plants,Beijing Linye Daxu Xuebao(Journal of Beijing Forestry University),25(5):47-50(石娟,王月,徐洪儒,闫国增,梁洪柱,张秋霜,2003,不同食料植物对舞毒蛾生长发育的影响,北京林业大学学报25(5):47-50)
Shinzato N.,Muramatsu M.,Matsui T.,and Watanabe Y.,2007Phylogenetic analysis of the gut bacterial microflora of the fungus-growing termite Odontotermes formosanus,Biosci Biotechnol.Biochem.,71(4):906-915
Sun D.L.,Jiang X.,Wu Q.L.,and Zhou N.Y.,2013,Intragenomi heterogeneity of 16S r RNA genes causes overestimation o prokaryotic diversity,Applied and Environment Microbiolo gy,79(19):5962-5969
Thiel M.,1999,Reproductive biology of a wood-boring isopod Sphaeroma terebrans,with extended parental care,Marine Biology,135(2):321-333
Wang S.Y.,2010,A generation of high throughput sequencing and its application prospect in clinical,Guangdong Yixue(Medical Journal),31(3):269-272(王升跃,2010,新一代高通量测序技术及其临床应用前景,广东医学,31(3)269-272)
Wang X.H.,Li H.R.,Zhang X.H.,Li Y.,Ji W.S.,and Xu H.S.2000,Microbial flora in the digestive tract of adult penaeid shrimp(Penaeus chinensis),Qingdao Haiyang Daxue Xueba(Journal of Ocean University of Qingdao),30(3):493-498(王祥红,李会荣,张晓华,李筠,纪伟尚,徐怀恕,2000,中国对虾成虾肠道微生物区系,青岛海洋大学学报,30(3)493-498)
Xiang H.,and Zhou Z.H.,2009,Lignocellulolytic enzymes in ter mite and its symbiotic microbes,Kunchong Zhishi(Chines Bulletin of Entomology),46(1):32-40(相辉,周志华,2009白蚁及共生微生物木质纤维素水解酶的种类,昆虫知识46(1):32-40)
Yu H.Y.,and Li X.Z.,2003,Study on the species of sphaeromati dae from Chinese waters,Haiyang Kexue Jikan(Studia Ma rina Since),(45):239-259(于海燕,李新正,2003,中国近海团水虱科种类记述,海洋科学集刊,(45):239-259)
Zhao K.,Chang Z.W.,Zhang X.Y.,Hao Y.,Wu T.,Ping W.X.and Zhou D.P.,2012,Recent advances in diversity of symbi otic microbes in termite gut and termite control methods Yingyong Yu Huanjing Shengwu Xuebao(Chinese Journa of Applied and Environmental Biology),18(2):331-337(赵凯,常志威,张小燕,郝妍,吴桐,平文祥,周东坡,2012,白蚁肠道共生微生物多样性及其防治方法研究现状,应用与环境生物学报,18(2):331-337)
Zhou X.G.,Smith J.A.,Oi F.M.,Koehler P.G.,Bennett G.W.and Scharf M.E.,2007,Correlation of cellulase gene expres sion and cellulolytic activity throughout the gut of the ter mite Reticulitermes flavipes,Gene,395(1-2):29-39
基本信息:
DOI：10.13417/j.gab.035.000406
中图分类号:Q938.1
引用信息:
[1]胡亚强,丁敬敬,黄勃,等.团水虱不同生长阶段肠道微生物多样性分析[J].基因组学与应用生物学,2016,35(02):406-413.DOI:10.13417/j.gab.035.000406.
基金信息:
国家自然科学基金项目(41176084);; 海南GEF项目;; 国家科技支撑计划项目(2009BADB2B0404-02);; 国家海洋公益项目(201105008-7);; 中医药行业科研专项(201207002-03);; 海南省重点科技项目(20080137);; 教育部海南大学中西部计划学科重点领域建设项目(ZXBJH-XK006);; 海南大学植物学国家重点学科课题(071001)共同资助
2016-02-25
2016-02-25