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为了研究烟草幼苗对弱光胁迫反应的分子机制,以‘云烟87’为材料,构建全光照和弱光处理条件下大十字期烟苗cDNA文库,利用Illumina测序技术进行转录组测序,筛选差异表达基因。结果表明:借助RNA-seq技术共筛选到2 956个DEGs,其中弱光相对于全光照表达上调的DEGs有691个,表达下调的DEGs为2 265个;通过GO功能分类和KEGG富集分析,将差异基因划分为30个功能类别和113个代谢途径,包括代谢进程、碳水化合物结合、核酸结合转录因子活性、水解酶活性、转录因子活性、催化活性、内质网蛋白质加工、植物-病原体互作、苯丙烷生物合成、苯丙氨酸代谢、胁迫反应、激素信号转导以及光合作用等。本研究将为揭示烟草对弱光胁迫反应的分子机制提供理论依据。
Abstract：In order to study the molecular mechanism of tobacco seedings response to low light intensity stress, a fully expanded cotyledon c DNA library was constructed under germination conditions at full and low light intensity during big cross seedling stage using tobacco variety 'Yunyan87' as the material. To screen differentially expressed genes(DEGs) of tobacco, the Illumina sequencing technique was used to carry out transcriptional sequencing(RNA-seq). The results showed that 2 956 DEGs were screened compared to full light intensity by RNA-seq,including 691 DEGs associated with up-regulation and 2 265 DEGs associated with down-regulation. Through GO functional classification and KEGG enrichment analysis, the differential genes were divided into 30 functional categories and 113 metabolic pathways. These include metabolic process, carbohydrate binding, catalytic activity, nucleic acid binding transcription factor activity, hydrolase activity, transcription factor activity, catalytic activity,protein processing in endoplasmic reticulum, plant-pathogen interaction, phenylpropanoid biosynthesis, phenylalanine metabolism, response to stress, hormone signal transduction and photosynthesis. This study will provide a theoretical basis for revealing the molecular mechanism of tobacco seedlings under low light intensity environment.
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基本信息:
DOI：10.13417/j.gab.040.000315
中图分类号:S572
引用信息:
[1]关晓溪,隋常玲,陈瑶,等.烟草幼苗响应弱光胁迫的转录组分析[J].基因组学与应用生物学,2021,40(01):315-324.DOI:10.13417/j.gab.040.000315.
基金信息:
遵义市科技局联合科技研发资金项目(遵市科合计[2018]10号); 遵义市创新人才团队培养项目(遵市科人才[2019]7号); 遵义师范学院服务农村产业革命项目(遵师合农村产业字201910);遵义师范学院博士基金资助项目(遵师BS[2015]14号)共同资助; 遵义师范学院2017学术新苗培养及创新探索专项项目培育项目(黔科合平台人才[2017年]5727-18号)
2019-12-04
2019-12-04
2019-12-04