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高活性且平衡的微生物群落结构是厌氧生化处理成功的关键。本研究将糖蜜酒精废水与猪粪水以COD比为3:2混合后,利用有工作积为20 L的IC厌氧反应器在自然温度(20~38℃)下进行厌氧发酵。在不添加任何营养元素以及缓冲物的条件下,最适进水负荷为3 200 mg/L,HRT为24 h,COD去除率最高约为80%,单日最高沼气产量为20.15 L。沼气产量与COD去除率呈正相关性。采用传统的分离培养法对发酵不同时期(启动期Q1,稳定期Q2,超负荷期Q3,调整期W1)的活性污泥中可培养菌进行分离纯化。通过比较发现4个阶段中好氧菌占主导地位的菌群分别为Pseudomonas(假单胞菌属)和Bacillus(芽孢杆菌属)(Q1)、Bacillus(芽孢杆菌属)(Q2)、Bacillus(芽孢杆菌属)和Advenella(产碱菌科)(Q3)、Rhodococcus(红球菌属)(W1);而厌氧菌中占主导地位的为Clostridium(梭菌属),不同阶段的污泥中微生物群落在属水平上的种类与数量均有差异。
Abstract：A balanced microbial community structure with high activity is crucial for anaerobic biological treatment. In this study, molasses alcohol wastewater mixed with pig manure with COD ratio of 3: 2. A 20 L IC anaerobic reactor was used and operated under natural temperature(20~38℃). Without adding any nutrients and buffers, the optimum OLR was 3 200 mg/L, and HRT was 24 h, the COD removal rate could reach up to 80%, the highest yield of biogas was 20.15 L daily. Biogas production and COD removal rate were positively correlated.Some culturable microorganisms were isolated and cultured from activated sludge of different periods(the starting stage(Q1), stable stage(Q2), overloaded stage(Q3) and adjustmental stage(W1)) with traditional methods. The results showed that the dominant aerobic bacteria were Pseudomonas and Bacillus(Q1), Bacillus(Q2), Bacillus and Advenella(Q3), Rhodococcus(W1), and the major anaerobic bacteria was Clostridium in the whole process. Microbial species and numbers were different in different stages.
Antonia M.J.,Rafael B.,and Antonio M.,2003,Aerobic-anaerobic biodegradation of beet molasses alcoholic fermentation waste-water,Process Biochemistry,38(9):1275-1284
Chen Z.L.,Cheng C.,Ma K.,Liu G.Q.,Li H.,and Li H.,2008,The isolation and identification of lactic acid bacteria in Yak fermented dairy products,Shipin Kexue(Food Science),29(12):408-412(陈芝兰,程池,马凯,刘光全,李红,李辉,2008,西藏地区牦牛发酵乳制品中乳酸菌的分离与鉴定,食品科学,29(12):408-412)
Martín-González L.,Font X.,and Vicent T.,2013,Alkalinity ratios to identify process imbalances in anaerobic digesters treating source-sorted organic fraction of municipal wastes,Biochemical Engineering Journal,76(15):1-5
Murugaragavan R.,and Mahimairaja S.,2009,Problems and prospects of agricultural use of distillery spentwash in India,Journal of Ecotoxicology and Environmental,19(4):375-382
Pant D.,and Adholeya A.,2007,Biological approaches for treatment of distillery wastewater:a review,Bioresource Technology,98(12):2321-2334
Satyawali Y.,and Balakrishnan M.,2008,Treatment of distillery effluent in a membrane bioreactor(MBR)equipped with mesh filter,Separation and Purification Technology,63(2):278-286
Shen P.H.,Han F.,Su S.Q.,Zhang J.Y.,Chen Z.N.,Li J.F.,Gan J.Y.,Feng B.,and Wu B.,2014,Using pig manure to promote fermentation of sugarcane molasses alcohol wastewater and its effects on micro bial community structure,Bioresource Technology,155:323-329
Shen P.H.,Zhang J.Y.,Zhang J.,Jiang C.J,Tang X.L.,Li J.F.,Zhang M.,and Wu B.,2013,Changes in microbial community structure in two anaerobic systems to treat bagasse spraying wastewater with and without addition of molasses alcohol wastewater,Bioresource Technology,131(2013):333-340
Subhankar B.,Sangeeta M.,Ankita K.,Vidya S.,and Batra M.B.,2015,Integrated treatment of molasses distillery wastewater using microfiltration(MF),Journal of Environmental Management,158:55-60
Tondee T.,Sirianuntapiboon S.,and Ohmomo S.,2008,Decolorization of molasses wastewater by yeast strain Issatchenkia orientalis No.SF9-246,Bioresource Technology,99(13):5511-5519
基本信息:
DOI：10.13417/j.gab.035.003437
中图分类号:X703;Q93
引用信息:
[1]耿三春,李园,卢铁东,等.小试厌氧混合处理糖蜜酒精废液及不同阶段活性污泥中可培养微生物的群落结构分析[J].基因组学与应用生物学,2016,35(12):3437-3444.DOI:10.13417/j.gab.035.003437.
基金信息:
广西科技计划项目(11107021-4)资助
2016-12-25
2016-12-25