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为了深入认识低能N+注入对细菌基因组的进化与突变的作用,本研究在低能N+注入介导外源基因转化沙漠寡营养细菌DOB150的基础上,通过生物信息学方法对3株重组突变的DOB基因组的单核苷酸多态性进行了分析,共发现192 357个SNP位点。其中重组突变菌株DOB073和DOB113的SNPs类型和数量基本相等,而重组突变菌株DOB981的SNPs类型丰富且数量大。DOB073和DOB113基因组中SNP数目约为0.005个/kb,DOB981约为37.620个/kb。在3株重组突变菌的基因组的SNPs中,AG转换所占比例均为最高。根据SNPs绘制的DOB基因组系统发育树显示,重组突变菌株DOB981的进化速度快于DOB073和DOB113。
Abstract：In order to find out about evolution and mutation process of Desert Oligotrophic Bacteria(DOB) by low energy N~+implanted, the 192 357 single nucleotide polymorphism sites(SNPs) were found by using Bioinformatics.The type of SNPs in recombinant mutant strain DOB073 and DOB113 were the same, and the number of SNPs were nearly equal, but the type of SNPs in recombinant mutant strain DOB981 were rich, while the number of SNPs were large. The number of SNPs in DOB073 and DOB113 genomes were about 0.005/kb, and in DOB981 genome were about 37.620/kb. In the result of SNPs in three strains, the AG transformation type had the highest percentage. As showned in the phylogenetic tree which was predicted by compared the results of SNPs, recombinant mutant strain DOB981 evolved faster than the DOB073 and DOB113.
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基本信息:
DOI：10.13417/j.gab.035.003425
中图分类号:Q933
引用信息:
[1]李黎,毛培宏.低能N~+注入介导的沙漠寡营养细菌基因组SNP研究[J].基因组学与应用生物学,2016,35(12):3425-3429.DOI:10.13417/j.gab.035.003425.
基金信息:
国家自然科学基金(11275164)资助
2016-12-25
2016-12-25