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采用室内模拟培养,研究了甲霜灵对映体对土壤基础呼吸、诱导呼吸的选择性影响,以及甲霜灵的两种异构体分别对土壤中脲酶活性和过氧化氢酶活性影响的差异性,探讨对土壤微生物总体活性的影响。甲霜灵对映体对土壤微生物总体活性均呈现相似的抑制-激活-恢复效应。土壤微生物抑制效应随着甲霜灵对映体浓度的增大而明显,即抑制存在剂量效应关系,在一定时期内不同构型对于微生物总体活性存在选择性影响,R-甲霜灵抑制效应更为显著;试验后期,高浓度的甲霜灵对映体能激活土壤微生物总体活性,但随后活性缓慢恢复至对照水平。结果表明甲霜灵对映体对土壤微生物总体活性的影响不仅与处理的浓度和培养时间有关,同时还存在立体选择性差异。这些研究成果为全面评价手性农药对土壤生态系统的影响供了一定的科学理论依据。
Abstract：The effects of metalaxyl enantiomers on soil basal respiration, induced respiration were studied by indoor simulated culture, and the difference of the effects of the two isomers of metalaxyl on the activity of the enzyme and the activity of catalase in the soil, respectively, in order to discuss the effect on the total activity of the soil microorganism. The effects of metalaxyl enantiomers on the total activity of soil microbes showed similar inhibition-activation-recovery effect. With the increase of metalaxyl enantiomer concentration, the inhibition effect of soil microorganism became more and more obvious, that is, there is a dose-effect relationship. During a certain period, different configurations have selective effects on the overall activity of microorganisms, and the inhibitory effect of R-metalaxyl is more significant; During the later incubation time, high concentration metalaxyl enantiomers can activate soil microbial activity, but then the activity slowly returned to the control level. The results showed that the effect of metalaxyl enantiomers on soil microbial activity was not only related to the concentration and time of culture, but also to the stereoselectivity. These provide a scientific theoretical basis for the comprehensive evaluation of the effect of the chiral pesticide on the soil ecosystem.
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基本信息:
DOI：10.13417/j.gab.038.004552
中图分类号:S154.3
引用信息:
[1]陶怡,方松,王允白.甲霜灵对映体对土壤微生物总体活性的选择性影响[J].基因组学与应用生物学,2019,38(10):4552-4556.DOI:10.13417/j.gab.038.004552.
基金信息:
中国农业科学院科技创新工程(ASTIP-TRIC06);; 国家农产品质量安全风险评估重大专项(GJFP2018010)共同资助
2018-06-05
2018-06-05
2018-06-05