华南农业大学农学院广东省微生物信号与作物病害防控重点实验室;
随着大量丝状真菌基因组序列的公布,丝状真菌基因功能研究已然成为现今分子生物学的热点领域。真菌的遗传转化是研究基因功能的重要手段,研究者不断优化和开发出新的、高效的遗传转化方法,以满足更多真菌物种研究的需要。本综述总结了丝状真菌的遗传转化体系的最新研究进展,如遗传转化方法、选择标记的类型和转化系统的应用。
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Bluhm B.H.,Zhao X.,Flah erty J.E.,Xu J.R.,and Dunkle L.D.,2007,RAS2 regulates growth and pathogenesis in Fusarium graminearum,Mol.Plant Microbe Interact,20(6):627-636
Bundock P.,Den D.A.,Beijersbergen A.,and Hooykaas P.J.,1995,Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae,Embo.J.,14(13):3206-3214
Cao Z.L.,Wang D.P.,and Zhang L.,2016,Improvement of transformation efficiency of Aspergillus niger mediated by Agrobacterium tumefaciens,Tianjin Kejidaxue Xuebao(Journal of Tianjin University of Science&Technology),31(2):20-25(曹张磊,王德培,张岚,2016,提高根癌农杆菌介导黑曲霉转化效率的研究,天津科技大学学报,31(2):20-25)
Chaure P.,Gurr S.J.,and Spanu P.,2000,Stable transformation of Erysiphe graminis an obligate biotrophic pathogen of barley,Nat.Biotechnol.,18(2):205-207
Chen Y.J.,and Wei J.F.,2009,Progress on the research of genetic transformation of filamentous fungi,Yunnan Nongyedaxue Xuebao(Journal of Yunnan Agricultural University),24(3):448-454(陈赟娟,韦建福,2009,丝状真菌遗传转化的研究进展,云南农业大学学报,24(3):448-454)
Christiansen S.K.,Knudsen S.,and Giese H.,1995,Biolistic transformation of the obligate plant pathogenic fungus,Erysiphe graminis f.sp.hordei,Curr.Genet.,29(1):100-102
Ding S.L.,Mehrabi R.,Koten C.,Kang Z.S.,Wei Y.D.,Seong K.Y.,Kistler H.C.,and Xu J.R.,2009,Transducin beta-like gene FTL1 is essential for pathogenesis in Fusarium graminearum,Eukaryot Cell,8(6):867-876
Dobinson K.F.,and Grant S.S.,2004,Cloning and targeted disruption,via Agrobacterium tumefaciens-mediated transformation,of a trypsin protease gene from the vascular wilt fungus Verticillium dahliae,Curr.Genet.,45(2):104-110
Feng X.,Lin C.H.,Kang X.,Jin Y.,Liu X.,He Q.G.,Liu W.B.,Miao W.G.,and Zheng F.C.,2017,Gene cloning and functional analysis of OhPbs2 in Oidium heveae,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),50(1):77-85(冯霞,林春花,康迅,金洋,刘晓,何其光,刘文波,缪卫国,郑服丛,2017,橡胶树白粉病菌Oh Pbs2的克隆及功能分析,中国农业科学,50(1):77-85)
Granado J.D.,Kertesz-ChaloupkováK.,Aebi M.,and Kües U.,1997,Restriction enzyme-mediated DNA integration in Coprinus cinereus,Mol.Genet.Genomics,256(1):28-36
Groot M.J.A.D.,Bundock P.,Hooykaas P.J.J.,and Beijersbergen A.G.M.,1998,Agrobacterium tumefaciens-mediated transformation of filamentous fungi,Nat.Biotechnol.,16(9):839-842
Han X.L.,Bai J.K.,Zhang W.,Zhang R.,and Sun G.Y.,2016,PEG-mediated transformation Colletotrichum fructicola,Xibei Nongye Xuebao(Acta Agriculturae Boreali-occidentalis Sinica),25(3):442-449(韩小路,白静科,张玮,张荣,孙广宇,2016,PEG介导的苹果果生刺盘孢Colletotrichum fructicola原生质体转化,西北农业学报,25(3):442-449)
Hinnen A.,Hicks J.B.,and Fink G.R.,1978,Transformation of yeast,P.Natl.Acad.Sci.Usa.,75(4):1929-1933
Huan Y.L.,Ye J.,Jiang X.L.,and Zhu C.X.,2007,Advance in genetic transformation system of fungi,Weishengwuxue Tongbao(Journal of Microbiology),34(6):1213-1217(黄亚丽,叶婧,蒋细良,朱昌雄,2007,真菌遗传转化系统的研究进展,微生物学通报,34(6):1213-1217)
Irie T.,Honda Y.,Watanabe T.,and Kuwahara M.,2001,Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA,Appl.Microbiol.Biot.,55(5):563-565
Katz M.E.,and Hynes M.J.,1989,Characterization of the amd R-controlled lamA and lamB genes of Aspergillus nidulans,Genetics,122(2):331-339
Kong D.Z.,Hua C.L.,Wang X.Z.,Liu B.C.,Zhu J.B.,and Wang A.Y.,2016,dGFP-GUS genes tagged of Verticillium dahliae of cotton in Xinjiang marking,Xinjiang Nongye Kexue(Xinjiang Agricultural Sciences),53(1):1-8(孔德真,华东来,王秀珍,刘步仓,祝建波,王爱英,2016,dGFP-GUS基因标记新疆棉花黄萎病原菌的研究,新疆农业科学,53(1):1-8)
Kong H.M.,2008,Agrobacterium tumefaciens-mediated transfor mation of Magnaporthe grisea,Thesis for M.S.,Zhejiang University,Supervisor:WANG Y.Z,pp.5-6(孔慧敏,2008,农杆菌介导的稻瘟病菌转化及致病突变体的筛选,硕士学位论文,浙江大学,导师:王逸政,pp.5-6)
Li H.Y.,Pan C.Y.,Chen H.,Zhao C.J.,Lu G.D.,and Wang Z.H.,2003,Optimization of T-DNA insertional mutagenesis and analysis of mutants of Magnaporthe grisea,Shengwu Gongcheng Xuebao(Chinese Journal of Biotechnology),19(4):419-423(李宏宇,潘初沂,陈涵,赵长江,鲁国东,王宗华,2003,稻瘟病菌T-DNA插入方法优化及其突变体分析,生物工程学报,19(4):419-423)
Li J.,Yang J.K.,Liang L.M.,and Zhang K.Q.,2006,Advance in the research on genetic transformation system of filamentous fungi,Jiangxi Nongyedaxue Xuebao(Acta Agriculturae U-niversitatis Jiangxiensis),28(4):516-520(李娟,杨金奎,梁连铭,张克勤,2006,丝状真菌遗传转化系统研究进展,江西农业大学学报,28(4):516-520)
Li L.,Xue C.,Bruno K.,Nishimura M.,and Xu J.R.,2004,Two PAK kinase genes,CHM1 and MST20,have distinct functions in Magnaporthe grisea,Mol.Plant Microbe In.17(5):547-556
Li S.Q.,Yi X.N.,Jia Y.J.,and Zhang C.X.,2014,A simple and high-efficient electroporation method for Penicillium chrysogenum,Zhongguo Kangshengsu Zazhi(Chinese Journal of Antibiotics),39(9):651-653(李树强,仪修南,贾亚娟,张春晓,2014,产黄青霉简单高效遗传转化法---电激转化法,中国抗生素杂志,39(9):651-653)
Li W.,and Zhang Y.Z.,2005,Agrobacterium tumefaciens-mediated transformation of the white-rot basidiomycete,Phanerochaete chrysosporium,Weishengwu Xuebao(Acta Microbiologica Sinica),45(5):784-787(李维,张义正,2005,根癌农杆菌介导的白腐丝状真菌-黄孢原毛平革菌的转化,微生物学报,45(5):784-787)
Liu F.,2016,Selection of medium for Aspergillus ochraceus and roles of AopacC gene involved in regulation of growth and toxin biosynthesis,Thesis for M.S.,Jiangsu University,Supervisor:Wang Y.,pp.46-50(刘菲,2016,赭曲霉培养基筛选及AopacC基因对生长和产毒调控的功能研究,硕士学位论文,江苏大学,导师:王云,pp.46-50)
Lu S.,Lyngholm L.,Yang G.,Bronson C.,Yoder O.C.,and Turgeon B.G.,1994,Tagged mutations at the Tox1 locus of Cochliobolus heterostrophus by restriction enzyme-mediated integration,P.Natl.Acad.Sci.Usa.,91(26):12649-12653
Malmierca M.G.,Cardoza R.E.,Alexander N.J.,Mccormick S.P.,Collado I.G.,Hermosa R.,Monte E.,and Gutiérrez S.,2013,Relevance of trichothecenes in fungal physiology:disruption of tri5 in Trichoderma arundinaceum,Fungal Genetics&Biology,53(2):22-33
Meyer V.,2008,Genetic engineering of filamentous fungi progress,obstacles and future trends,Biotechnol.Adv.,26(2):177-185
Meyer V.,Arentshorst M.,Elghezal A.,Drews A.C.,Kooistra R.,Ca V.D.H.,and Ram A.F.,2007,Highly efficient gene targeting in the Aspergillus niger kusA mutant,J.Biotechnol.,128(4):770-775
Michielse C.B.,Hooykaas P.J.,Ca V.D.H.,and Ram A.F.,2005,Agrobacterium-mediated transformation as a tool for functional genomics in fungi.Current,Genetics,48(1):1-17
Monschau N.,Sahm H.,and Stahmann K.P.,1998,Threonine aldolase over expression plus threonine supplementation enhanced riboflavin production in Ashbya gossypii,Applied&Environmental Microbiology,64(11):4283-4290
Robinson M.,and Sharon A.,1999,Transformation of the bioherbicide Colletotrichum gloeosporioides f.sp.aeschynomene by electroporation of germinated conidia,Curr.Genet.,36(1):98-104
Ruiz-Díez B.,2002,Strategies for the transformation of filamentous fungi,J.Appl.Microbiol.,92(2):189
Sánchez O.,Navarro R.E.,and Aguirre J.,1998,Increased transformation frequency and tagging of developmental genes in Aspergillus nidulans by restriction enzyme-mediated integration(REMI),Mol.Genet.Genomics,258(1):89-94
Schaaf A.,Langbein S.,Knoll T.,Alken P.,and Michel M.S.,2003,In vitro transfection of human bladder cancer cells by acoustic energy,Anticancer Res.,23(23):4871-4875
Sun X.W.,2016,Morphology optimization of Aspergillus niger by regulating chitin synthase gene,Thesis for M.S.,Anhui University,Supervisor:Zheng Z.M.,pp.50-54(孙小雯,2016,基于几丁质合成酶基因调控的黑曲霉发酵形态优化,硕士学位论文,安徽大学,导师:郑之明,pp.50-54)
Ward O.P.,2012,Production of recombinant proteins by filamentous fungi,Biotechnol.Adv.,30(5):1119-1139
Wang H.,2013,The priliminary study on the genetic operation of Phanerochaete chrysosporium and expression of manganese peroxidase gene in the Trichoderma reesei,Thesis for M.S.,Shandong University,Supervisor:Lu X.M.,pp.25-26(王慧,2013,黄孢原毛平革菌遗传转化初探和锰过氧化物酶基因在瑞氏木霉中的表达,硕士学位论文,山东大学,导师:卢雪梅,pp.25-26)
Wang Z.Y.,Wang Q.H.,and Li D.B.,2003,Agrobacterium tumefaciens mediated transformation filamentous fungi,Junwu Xuebao(Journal of Bacteria),22(2):339-344(王政逸,王秋华,李德葆,2003,根癌土壤杆菌介导的丝状真菌转化,菌物学报,22(2):339-344)
Wei Q.Y.,2015,Study on the transgenic wheat with fusarium head blight resistace-related genes by particle bombardment,Thesis for M.S.,Huazhong Agricultural University,Supervisor:Li H.P.,pp.43-47(韦奇勇,2015,基因枪介导的转抗赤霉病相关基因小麦的研究,硕士学位论文,华中农业大学,导师:李和平,pp.43-47)
Xu Q.J.,Hu X.P.,Chen T.,and Zhao J.X.,2012,Protoplast transformation of Fusarium oxysporum f.sp.vasinfectum mediated by polyethylene glyol,Mianhua Xuebao(Cotton Science),24(3):222-228(徐齐君,胡小平,陈婷,赵俊兴,2012,PEG介导的棉花枯萎病菌原生质体转化体系的建立,棉花学报,24(3):222-228)
Yang H.,2010,Genetic diversity of Magnaporthe grisea from rice in Jiangsu and identification of resistance rice blast gene,Thesis for M.S.,Nanjing Agricultural University,Supervisor:Zhou Y.J.,pp.13-15(杨豪,2010,江苏省稻瘟病菌遗传多样性及水稻抗瘟基因鉴定,硕士学位论文,南京农业大学,导师:周益军,pp.13-15)
Yang Y.Q.,Li M.H.,Yang M.,Li Y.,and Zhou E.X.,2010,Optimized conditions for the preparation and regeneration of protoplasts from Rhizoctonia solani Kǜhn AG-1 IA,Huazhong Nongyedaxue Xuebao(Journal of Huazhong Agricultural U-niversity),29(5):546-551(杨迎青,李明海,杨媚,李勇,周而勋,2010,水稻纹枯病菌原生质体制备与再生条件的优化,华中农业大学学报,29(5):546-551)
Zhang J.H.,Mou M.,Chang H.,Li Y.P.,Fan L.,Zhong Q.Y.,Pan C.Q.,and Zou D.T.,2016,Agrobacterium tumefaciens-mediated transformation of gfp gene and its effects on stability and pathogenicity for Rhizoctonia solani,Huazhong Nongye Kexue(Journal of Northeast Agricultural Sciences),6(41):67-74(张俊华,牟明,常浩,李云鹏,范琳,钟庆燕,潘春清,邹徳堂,2016,根癌农杆菌介导gfp基因转化水稻纹枯病菌及其对病原菌稳定性和致病力的影响,东北农业科学,6(41):67-74)
Zhang Z.G.,and Li D.P.,1998,Protoplast technique of filamentous fungi,Hunan Shifan Daxue Zirankexue Xuebao(Journal of Natural Science of Hunan Normal University),3(1):60-65(张志光,李东屏,1998,丝状真菌原生质体技术研究,湖南师范大学自然科学学报,3(1):60-65)
Zhou L.H.,Chen P.,Zhao Y.X.,Jing W.W.,Li Z.,and Ge Y.Y.,2012,Optimizing of genetic transformation conditions from Monascus anka by restriction enzyme-mediated DNA integration(REMI),Hubei Nongye Kexue(Hubei Agricultural Sciences),51(18):4129-4133(周礼红,陈平,赵永霞,荆雯雯,李祝,葛永仪,2012,REMI介导红曲霉遗传转化条件的优化,湖北农业科学,51(18):4129-4133)
Zhou L.H.,Li G.Q.,Wang Z.X.,and Zhu G.J.,2005,Preparation and regeneration of protoplasts from Monascus purpureus and genetic transformation system,Yichuan(Hereditas),27(3):423-428(周礼红,李国琴,王正祥,诸葛健,2005,红曲霉原生质体的制备、再生及其遗传转化系统,遗传,27(3):423-428)
基本信息:
DOI:10.13417/j.gab.038.001138
中图分类号:Q933
引用信息:
[1]赵美,王陈骄子,周而勋等.丝状真菌遗传转化系统的最新研究进展[J].基因组学与应用生物学,2019,38(03):1138-1143.DOI:10.13417/j.gab.038.001138.
基金信息:
广东省自然科学基金(博士启动)项目(2016A030310454)资助