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以贵州宗地花猪和贵州从江香猪2个地方猪种为研究对象,构建基因组DNA池,扩增GDF9基因第1外显子和第2外显子序列,采用直接测序法对2个品种的GDF9基因进行单核苷酸多态性进行检测,利用生物信息学软件预测不同多态性位点对GDF9基因m RNA二级结构、蛋白质二级结构的影响。结果表明,在2个品种中共检测到T997A、C1009T、T1014C、G1137T和A1196T 5个SNPs位点,其全位于第二外显子上。T997A、C1009T和A1196T均为错义突变。T997A导致编码的苯丙氨酸(Phe)变为异亮氨酸(Ile)、C1009T导致编码的亮氨酸(Leu)变为苯丙氨酸(Phe)、A1196T导致编码的赖氨酸(Lys)变为甲硫氨酸(Met)。SNPs位点对于GDF9基因的m RNA二级结构、蛋白质二级结构有一定影响。
Abstract：In this study, Zongdihua pig and Congjian pig from two local breeds of Guizhou were selected as experimental materials. By constructing DNA pools to amplify the all sequences of extron 1 and extron 2 on GDF9 gene, taking direct sequencing method to detect the single nucleotide polymorphisms on GDF9 gene of two varieties, the effects of different polymorphisms loci on m RNA and protein secondary structure of GDF9 gene were predicted by bioinformatics software. The results presented that 5 SNPs including T997 A, C1009 T, T1014 C, G1137 T and A1196 T were discovered to locate in extron 2, and among these SNPs, T997 A, C1009 T, and A1196 T were missense mutation, T997 A mutation leaded to phenylalanine(Phe) into isoleucine(Ile), C1009 T mutation leaded to leucine(Leu)into phenylalanine(Phe), A1196 T mutation leaded to lysine(Lys) into methionine(Met). SNPs sites might have some effects on m RNA secondary structure and protein secondary structure of GDF9 gene.
Cepica S.,Procházka R.,CivánováK.,Knoll A.,NěmcováL.,Masopust M.,KubíckováS.,MusilováP.,and Rube觢J.,2004,Partial molecular characterization and mapping of the GDF9 gene to porcine chromosome 2,Anim.Genet.,35(3):261-262
China National Commission of Animal Genetic Resources,ed.,2011,Animal genetic resources in China.Poultry-Pig,Beijing,China,pp.321-324(国家畜禽遗传资源委员会,主编,2011,中国畜禽遗传资源志.猪志,中国农业出版社,中国,北京,pp.321-324)
Cui J.X.,Du H.L.,and Zhang X.Q.,2005,Rapidly screening SNPs and estimating allelic frequencies by DNA pooling and sequencing,Yichuan Xuebao(Journal of Genetics and Genomics),32(4):372-377(崔建勋,杜红丽,张细权,2005,利用DNA池和测序技术快速筛查SNPs及估算基因频率,遗传学报,32(4):372-377)
Du H.L.,Chen J.,Zhang Y.S.,Cui J.X.,and Zhang X.Q.,2008,Expression differences in genes related to reproduction between Erhualian and Duroc Pigs,Huanan Nongye Daxue Xuebao(South China Agricultural University),29(2):99-103(杜红丽,陈静,张玉山,崔建勋,张细权,2008,二花脸与杜洛克猪繁殖相关基因表达差异,华南农业大学学报,29(2):99-103)
Elvin J.A.,Yan C.,and Mabuk M.M.,2002,Oocyte-exprssd TGF-βsuperfamily members in female fertility,Mol.Cell Endocrinol.,187:5-9
Feng W.H.,Yan Z.H.,and Mao T.H.,2008,Carcass and meat quality traits measured preliminary report of Zongdihua Pigs in Guizhou,Guizhou Xumu Shouyi(Guizhou Animal Science and Veterinary Medicine),32(2):9-10(冯文豪,燕志宏,毛同辉,2008,贵州宗地花猪胴体肉质性状测定初报,贵州畜牧兽医,32(2):9-10)
Incerti B.,Dong J.,Borsani G.,and Matzuk M.M.,1994,Sturcutre of the mouse growth/differentiation factor 9 gene,Biochim.Biophys.Acta,1222(l):125-128
Mao T.H.,Yan Z.H.,and Feng W.H.,Determination of the reproductive performance of the conservation and breeding bace population of Zongdihua Pig,(Guizhou Xvmu Shouyi),33(3):7-8(毛同辉,燕志宏,冯文豪,2009,宗地花猪产区保种选育基础群繁殖性能测定,贵州畜牧兽医,33(3):7-8)
Meng H.L.,2012,Gene polymorphism of GDF9,BMP15,BMPR-IB,OPN genes and their correlation with reproductive traits in pig,Dissertation for Ph.D.,Fujian Agriculture and Forestry University,Supervisor:Lin J.R.,pp.18-21(孟洪莉,2012,猪繁殖相关基因多态性检测及其关联分析,硕士学位论文,福建农林大学,导师:林洁荣,pp.18-21)
Sadighi M.,Bodensteiner K.J.,Beattie A.E.,and Galloway S.M.,2002,Genetic mapping of ovine growth differentiation factor-9(GDF9)to sheep chromosome 5,Anim.Genet.,33(3):244-245
Yang H.,Sun J.,Qiu G.H.,Song R.M.,Yang K.M.,Zhu M.G.,Li J.,and Zhang Y.,2014,Polymerphism detection and bioinformatics analysis of NKX2-5 gene of three local chicken breeds in Guizhou,(Zhongguo Xvmu Shouyi),12:209-214(杨红,孙鹃,裘贵华,宋汝谋,杨开明,朱明贵,李俊,张勇,2014,3个贵州地方鸡种NKX2-5基因多态性及生物信息学分析,中国畜牧兽医,12:209-214)
Yang J.L.,Zhang C.P.,Li L.,Huang L.,Yang J.L.,and Lu C.L.,2010Testosterone induces redistribution of forkhead box-3a and down-regulation of growth and differentiation factor 9 messenger ribonucleic acid expression at early stage of mouse folliculogenesis,Endocrinology,151(2):774-782
Yoon S.J.,Shin C.S.,and Baek K.H.,2000,Comparison of the GDF9 m RNA and protein expression between in vivo and in vitro cultured mouse ovaries,Fertil.Steril.,74:194-195
Zhang L.,Cao H.Z.,Lu C.L.,and Hu C.B.,2009,An Alu insertion polymophism in the porcine FSHβand GDF9 gene,(Xvmu Shouyi Xuebao),44(4):481-486(张璐,曹洪战,芦春莲,胡成波,2009,猪FSHβ和GDF9基因中Alu插入序列多态性,畜牧兽医学报,44(4):481-486)
基本信息:
DOI：10.13417/j.gab.034.002402
中图分类号:S828
引用信息:
[1]任丽群,燕志宏,顾丽菊,等.贵州宗地花猪的GDF9基因多态性及生物信息学分析[J].基因组学与应用生物学,2015,34(11):2402-2407.DOI:10.13417/j.gab.034.002402.
基金信息:
贵州省科技厅农业攻关项目(20123013,20127032)资助
2015-12-08
2015-12-08
2015-12-08