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本研究旨在探究海南文昌海域稀杯盔形珊瑚(Galaxea astreata)、角蜂巢珊瑚(Favites complanata)、滨珊瑚(Porites lichen)、瘿叶蔷薇珊瑚(Montipora aequituberculata)以及叶状蔷薇珊瑚(Montipora foliosa)共附生放线菌物种多样性。本研究提取珊瑚样品总DNA以扩增16S rRNA基因的V3-V4高变区，通过Miseq高通量测序对珊瑚的共附生放线菌群落结构和多样性进行研究分析。结果表明，6个珊瑚样品中存在丰富的放线菌类群，选取的放线菌门序列归为656个不同的OTU，包括放线菌门下的6个纲，19个目，33个科以及50个属。此外在6个珊瑚样品中均检测到大量的未知类群，有407个OTU没有指定到已知的分类水平，其中最高的是叶状蔷薇珊瑚及其共附生放线菌文库，未分类的属达到了94.17%，很可能代表着新的放线菌类群。多样性指数分析结果显示，稀杯盔形珊瑚的共附生微生物丰度和多样性水平最高，叶状蔷薇珊瑚为最低。本研究发现，珊瑚样品之间存在明显的共附生放线菌类群差异性，这种差异性不仅存在于珊瑚的种类之间，也表现在同种珊瑚的个体之间。
Abstract：The aim of this research is to study the diversity of Actinomycetes associated with corals Galaxea astreata, Favites complanata, Porites lichen, Montipora aequituberculata and Montipora foliosa from Wenchang sea area of Hainan. The genomic DNA of coral samples was extracted to amplify the V3-V4 hypervariable regions of the 16S rRNA gene that was sequenced with Illumina MiSeq platform to analyze the diversity and communities of Actinomycetes associated with the corals. There were abundant Actinomycetes groups in the corals,the selected sequences of Actinomycetes were grouped into 656 distinct OTUs, including 6 classes, 19 orders, 33families and 50 genera. In addition, a large number of unknown groups were detected in all corals and 407 OTUs were not assigned to a known taxonomic level, which may represent the new Actinomycetes groups and the highest of which was coral Montipora foliosa with 94.17% of unknown groups. The results of diversity index analysis showed that the coral Galaxea astreata possessed the highest level of diversity, and the lowest was the coral Montipora foliosa. There were obvious differences in coincidental Actinomycetes groups among different coral samples, which existed not only among species of corals but also among individual corals.
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基本信息:
DOI：10.13417/j.gab.040.003528
中图分类号:Q958.8
引用信息:
[1]李芬发,周双清,许云,等.海南文昌海域珊瑚非培养共附生放线菌的多样性[J].基因组学与应用生物学,2021,40(Z4):3528-3537.DOI:10.13417/j.gab.040.003528.
基金信息:
2019年海南省基础与应用基础研究计划（自然科学领域）高层次人才项目（2019RC069; 2019RC133）共同资助
2021-05-24
2021-05-24
2021-05-24