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杉木(Cunninghamia lanceolata)是我国南方主要的用材树种，具有广泛的用途。MYB转录因子家族在植物的生长、发育、非生物胁迫应答以及类黄酮生物合成等方面起到重要的调控作用。本研究克隆了与拟南芥(Arabidopsis thaliana)AtMYB5基因同源的杉木ClMYB5基因cDNA,对其进行生物信息学、表达特性及互作蛋白分析。生物信息学分析结果显示，ClMYB5蛋白为既亲水又亲脂蛋白，定位在细胞核中，有信号肽，无跨膜结构域，含有一个R2-R3结构域，属于R2R3-MYB转录因子亚家族；RT-PCR结果显示，ClMYB5在杉木根表达量最高，其次为树干木质部；酵母双杂交实验表明，ClMYB5与ClbHLH30蛋白存在相互作用。通过荧光素酶蛋白互补实验，进一步验证了ClMYB5与ClbHLH30存在互作关系。RT-PCR结果显示，ClbHLH30在形成层中表达量最高。综合实验结果表明，ClMYB5主要在杉木木质部维管组织中表达，在杉木类黄酮生物合成中可能具有潜在的调控功能。本研究增进了对裸子植物MYB转录因子家族成员作用的认知。
Abstract：Cunninghamia lanceolata is a major timber tree species in southern China with a wide range of uses.The MYB family of transcription factors plays an important regulatory role in plant growth, development, abiotic stress response, and flavonoid biosynthesis. In this study, we cloned the cDNA of Cunninghamia lanceolata ClMYB5 gene, which is homologous to the Arabidopsis thaliana AtMYB5 gene, and its bioinformatics prediction, expression pattern analysis, and reciprocal proteins were performed. The results of bioinformatics analysis speculated that the ClMYB5 protein is a hydrophilic and lipophilic protein, localised in the nucleus, has a signal peptide, no transmembrane structural domains, contains an R2-R3 structural domain, and belongs to the subfamily of R2R3-MYB transcription factors; RT-PCR results showed that ClMYB5 expression was highest in Cunninghamia lanceolata roots, followed by xylem.The results of yeast two-hybridisation indicated that ClMYB5 interacted with ClbHLH30 protein, and the results of luciferase complementation assay verified the existence of interactions between ClMYB5 and ClbHLH30. RT-PCR results showed that ClbHLH30 was most highly expressed in the formation layer. Comprehensive experimental results indicate that ClMYB5 is mainly expressed in the vascular tissues of Cunninghamia lanceolata xylem, and has a potential regulatory function in the biosynthesis of Cunninghamia lanceolata flavonoids. This study enhances the knowledge of the role of MYB transcription factor family members in gymnosperms.
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基本信息:
DOI：10.13417/j.gab.044.000345
中图分类号:Q943.2;S791.27
引用信息:
[1]李翠萍,张子健,文娅婷,等.杉木ClMYB5基因cDNA的克隆、表达特性与互作蛋白研究[J].基因组学与应用生物学,2025,44(04):345-354.DOI:10.13417/j.gab.044.000345.
基金信息:
国家自然科学基金项目(32060352); 国家重点研发计划子课题(2022YFD2200201-5); 广西大学科研启动基金项目(A3360051020)共同资助
2025-04-25
2025-04-25