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通过敲除球孢白僵菌(Beauveria bassiana)菌株bm01的PacC基因,研究了PacC基因敲除对球孢白僵菌毒力和分生孢子萌发速率的影响。利用农杆菌(Agrobacterium tumefaciens)介导的转化方法转化Bb_bm01,通过同源重组敲除Bb_bm01的PacC基因,筛选ΔPacC基因敲除菌株,获得了遗传稳定的ΔPacC基因敲除菌株。通过毒力测定,对比了野生菌株和ΔPacC基因敲除菌株对家蚕和大蜡螟毒力的差异,发现ΔPacC基因敲除菌株对家蚕和大蜡螟毒力都减弱,差异具有统计学意义(p<0.01),表明在Bb_bm01菌株中的PacC基因与它的致病性相关;另外,比较ΔPacC基因敲除菌株与野生型菌株Bb_bm01在SDB液体培养基中的孢子萌发率,发现ΔPacC基因敲除菌株孢子萌发明显减慢,表明PacC基因参与调控孢子萌发。
Abstract：By deletion the PacC gene in Beauveria bassiana bm01, the effect of PacC deletion on toxicity determination and conidia germination rate was assayed. The gene PacC in Bb_bm01 was deleted by homologous recombination, subsequently toxicity experiments to silkworm and Galleria mellonella were carried out and conidia germination rate was compared between wild type(WT) and ΔPacC deletion strain. A stable PacC deletion strain was obtained, which showed significantly reduced in the toxicity compared with WT, indicating that there was obvious effect on the toxicity when PacC was deleted. Moreover, the conidia germinated in SDB with lower rates than WT, indicating that the PacC gene in Bb_bm01 involved in the regulation conidia germination.
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基本信息:
DOI：10.13417/j.gab.035.002064
中图分类号:S476.12
引用信息:
[1]翟素珍,王吉平,陈晶晶,等.球孢白僵菌Bb_bm01菌株PacC基因突变体的构建及其毒力和孢子萌发率的测定[J].基因组学与应用生物学,2016,35(08):2064-2070.DOI:10.13417/j.gab.035.002064.
基金信息:
贵州省科技厅项目黔科合LH字[2014]7085号基金;; 贵州省教育厅项目黔教合KY[2012]39号基金共同资助
2016-08-25
2016-08-25