基因组学与应用生物学

本研究旨在研究未受运动训练的女学生,进行有氧游泳运动后,补充谷氨酰胺对于恢复期血液中一氧化氮(nitric oxide,NO)与同型半胱胺酸的影响。选取60位健康的女性大学生为研究对象,随机分成两组,一组补充安慰剂,一组补充L-谷氨酰胺,补充剂量皆为0.1 g每kg体重。然后进行单次游泳运动,连续运动2 h,运动强度为55%~75%最大心率。每位受试者在运动前及运动后0 h、1 h、2 h,分别采血以测量一氧化氮(NO_x,NO₂~-,NO₃~-)、同型半胱胺酸、血糖、胰岛素、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)、甘油三脂及总胆固醇含量。研究显示,运动后两组间受试者血液中一氧化氮、胰岛素、HDL-C、LDL-C、甘油三脂及总胆固醇含量并没有显著性差异,而补充谷氨酰胺组受试者运动后其血液中同型半胱胺酸及葡萄糖浓度水平升高。因此本研究认为谷氨酰胺能够帮助提高运动后恢复期血液中同型半胱胺酸及血糖的含量水平,但目前还没有足够证据能够显示补充谷氨酰胺能够增加耐力运动恢复期的一氧化氮水平。

This study aimed to investigate the effects of glutamine supplementation on nitric oxide(NO) and homocysteine in blood during recovery after aerobic swimming in untrained female students. Sixty healthy female university students were selected as the research object, who were randomly divided into two groups. One group received placebo or and the other received L-glutamine, with the supplement doses at 0.1 g/kg-wt. Then the students performed a single bout of aerobic swimming at an estimated speed corresponding to the 55% ~75%HRmax for 2 hours. Blood samples from each student were collected before exercise, and at 0 h, 1 h and 2 h after exercise to detect the concentrations of NO( NO_x, NO₂~-, NO₃~-), homocysteine, glucose, insulin, HDL-C( high-density lipoprotein cholesterol), LDL-C( low-density lipoprotein cholesterol), triglycerol, and total cholesterol in blood. The results showed that there were no differences in the levels of NOx, insulin, HDL-C,LDL-C, triglycerol, and total cholesterol between the groups after swimming. However, the concentrations of homocysteine and glucose were increased in the glutamine group after swimming. In conclusion, this study suggested that glutamine could increase the level of homocysteine and blood glucose in the blood at the recovery stage after exercise, but there was not enough evidence to demonstrate that glutamine supplementation couldincrease levels of nitric oxide in endurance exercise recovery.