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为了解二毛期时滩羊串子花型裘皮与其它类型裘皮中蛋白质的差异,本试验采用iTRAQ技术及LC-MS/MS蛋白质组学研究方法,对串子花型、软大花型、绿豆丝型及其它不规则型花穗裘皮蛋白质进行鉴定和筛选,并运用Proteome Discoverer l.4软件进行定量分析,结合数据库搜索,鉴定出具有显著表达差异的蛋白,同时应用生物学技术对其进行GO和Pathway分析。结果显示4类花穗型裘皮共检测出2 886个蛋白,其中有135个、142个、113个差异蛋白分别存在于软大花型与串子花型、绿豆丝型与串子花型、其它不规则型与串子花型3个对比组中。对有表达差异的蛋白进行分析,发现膜联蛋白与血管内皮生长因子可能与软大花型毛股形成相关,KAP3和KAP6可能与滩羊串子花型毛股结构相关。研究发现滩羊不同二毛裘皮蛋白水平上的差异,可为选育优良的滩羊串子花型二毛裘皮提供理论基础。
Abstract：In order to realize the difference between the expression of the protein in the er-mao ChuanZiHua type and other different types of fur,LC-MS/MS and iTRAQ proteomics methods were used to identify and screen the flower fur proteins on the ChuanZiHua type,RuanDaHua type,Lv DouSi type,other irregularities type.Proteome Discoverer l.4 software was used to study the quantitative analysis.In conjunction with database searching,the differential protein was identified by database search and GO and Pathway analysis were used to study the differential protein.A total of 2 886 proteins were identified in the four kinds of spike-type fur,of which 135,142 and 113 different proteins existed in the three contrast groups,includeing RuanDaHua type VS ChuanZiHua type,Lv DouSi type VS ChuanZiHua type and other irregularities type VS ChuanZiHua type.The expression of differential protein was analyzed.It was found that Annexin and vascular endothelial growth factor may be related to the formation of RuanDaHua type.KAP3 and KAP6 may be related to the structure of ChuanZiHua type of Tan sheep.It was found that the difference in protein level of Tan sheep's er-mao fur provided the theoretical basis for the study of the development of Tan sheep ChuanZiHua type.
Aguago A.,Kantarjian H.M.,Estey E.H.,Giles F.J.,Verstovsek S.,Manshouri T.,Gidel C.,O'Brien S.,Keating M.J.,and Albitar M.,2002,Plasma vascular endothelial growth factor levels have prognostic significance in patients with acute myeloid leukemia but not in patients with myelodysplastic syndromes,Cancer,95(9):1923-1930
Araújo V.R.,Silva G.M.,Duarte A.B.,Magalhúes D.M.,Almeida A.P.,Gon觔alves R.F.,Bruno J.B.,Silva T.F.,Campello C.C.,Rodrigues A.P.,and Figueiredo J.R.,2011,Vascular endothelial growth factor-A165(VEGF-A165)stimulates the in vitro development and oocyte competence of goat preantral follicles,Cell Tissue Res.,346(2):273-281
Bruno J.B.,Celestino J.J.,Lima-Verde I.B.,Lima L.F.,Matos M.H.T.,Araújo V.R.,Saraiva M.V.A.,Martins F.S.,Name K.P.O.,Campello C.C.,Báo S.N.,Silva J.R.V.,and Figueiredo J.R.,2009,Expression of vascular endothelial growth factor(VEGF)receptor in goat ovaries and improvement of in vitro caprine preantral follicle survival and growth with VEGF,Reproduction,Fertility and Development,21(5):679-687
Cockett N.E.,Shay T.L.,and Smit M.,2001,Analysis of the sheep genome,Physiological Genomics,7(2):69-78
Gao J.J.,Xie T.T.,Li S.W.,and Wang H.J.,2014,Prokaryotic expression and bioinformation analysis of wool follicle KAP7 of Hetian sheep,Zhongguo Xumu Zazhi(Chinese Journal of Animal Science),50(21):63-68(高建军,谢婷婷,李树伟,王海军,2014,和田羊毛囊KAP7基因的原核表达及其生物信息学分析,中国畜牧杂志,50(21):63-68)
Gu B.,Sun L.M.,Chang Q.,and Jiang H.Z.,2013,Study on VEGF in the expression of skin hair follicle and the relationship with microvessel density by liaoning cashmere goat in fetal period,Zhongguo Xumu Shouyi(China Animal Husbandry&Veterinary Medicine),40(6):158-161(谷博,孙丽敏,常青,姜怀志,2013,血管内皮生长因子基因在辽宁绒山羊胎儿期皮肤毛囊发育中表达及其与微血管密度关系的研究,中国畜牧兽医,40(6):158-161)
Hastie C.,Masters J.R.,Moss S.E.,and Naaby-Hansen S.,2008,Interferon reduces cell surface expression of Annexin2 and suppresses the invasive capacity of prostate cancer cells,Biol.Chem.,283(18):12595-12603
Jalali S.,del Pozo M.A.,Chen K.,Miao H.,Li Y.S.,Schwartz M.A.,Shyy J.Y.J.,and Chien S.,2001,Integrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix(ECM)ligands,Proc.Nat.Acad.Sci.,98(3):1042-1046
Jiang H.Z.,Chen Y.,and Chang Q.,2010,Effect of vascular endothelial growth factor(VEGF)on regulation of angiogenesis around skin follicle of mammal,Zhongguo Xumu Shouyi(China Animal Husbandry&Veterinary Medicine),37(5):47-49(姜怀志,陈洋,常青,2010,血管内皮生长因子在哺乳动物皮肤毛囊周围血管新生过程中的调控作用,中国畜牧兽医,37(5):47-49)
Jiang H.Z.,2012,Hair follicle structure traits and developmental mechanisms of Chinese cashmere goat,Jilin Nongye Daxue Xuebao(Journal of Jilin Agricultural University),34(5):473-482(姜怀志,2012,中国绒山羊的毛囊结构特性与发育机制,吉林农业大学学报,34(5):473-482)
Li J.B.,Yu C.H.,Guo N.N.,Tang J.,Liu Y.,and Zhang J.H.,2015,Research of enzyme-linked protein A2 and cathepsin B expression in non-small cell lung cancer,Linchuang Feike Zazhi(Journal of Clinical Pulmonary Medicine),20(5):815-817(李璟波,于长海,郭楠楠,唐健,刘颖,张建华,2015,膜联蛋白A2及组织蛋白酶B在肺鳞癌和腺癌中表达的研究,临床肺科杂志,20(5):815-817)
Liu C.J.,Fu X.F.,Tian K.C.,Huang X.X.,Xu X.M.,Zhan Z.H.,and Yu L.J.,2015,Screening and expression of keratin ESTin Xinji fine wool sheep,Zhongguo Xumu Shouyi(China Animal Husbandry&Veterinary Medicine),42(11):2873-2879(刘春洁,付雪峰,田可川,黄锡霞,徐新明,詹振宏,于丽娟,2015,新吉细毛羊角蛋白的EST筛选及表达研究,中国畜牧兽医,42(11):2873-2879)
Liu G.F.,Tian K.C.,Zhang E.P.,Huang X.X.,and Zhang Y.H.,2007,Candidate gene analysis of high quality merino sheep,Yichuan(Genetics),29(1):70-74(刘桂芬,田可川,张恩平,黄锡霞,张艳华,2007,优质细毛羊羊毛细度的候选基因分析,遗传,29(1):70-74)
Liu H.Y.,2007,Influence of nucleotide variations in KAP8,KA-P6.3 and FGF5 genes on fibre traits and change law of fibre quality,Dissertation for Ph.D.,China Agricultural University,Supervisor:Jia Z.H.,pp.61-79(刘海英,2007,KAP8,KAP6.3和FGF5基因变异对绒毛性状的影响及绒毛品质变化规律的研究,博士学位论文,中国农业大学,导师:贾志海,pp.61-79)
Liu Y.X.,Shi G.Q.,Wang H.X.,Wan P.C.,Tang H.,Yang H.,and Guan F.,2014,Polymorphisms of KAP6,KAP7,and KAP8 genes in four Chinese sheep breeds,Gene.Mol.Res.,13(2):3438-3445
Nash A.D.,Baca M.,Wright C.,and Scotney P.D.,2006,The biology of vascular endothelial growth factor-B(VEGF-B),Pulm.Pharmacol.Ther.,19(1):61-69
Nie J.Q.,Wang G.Z.,and Liu C.Q.,2012,Prokaryotic expression and purification of the human annexinA2 protein and preparation of its polyclonal antibody,Zhongguo Mianyixue Zazhi(Chinese Journal of Immunology),28(1):67-70(聂纪芹,汪龚泽,刘朝奇,2012,annexinA2基因片段的克隆,表达,纯化及多克隆抗体的制备,中国免疫学杂志,28(1):67-70)
Plowman J.E.,Debchoudhury S.,Bryson W.G.,Clerens S.,and Dyer J.M.,2009,Protein expression in orthocortical and paracortical cells of merino wool fibers,J.Agric.Food Chem.,57(6):2174-2180
Plowman J.E.,Harland D.P.,Ganeshan S.,Woods J.L.,Van Shaijik B.,Deb-Choudhury S.,Thomas A.,Clerens S.,and Scobie D.R.,2015,The proteomics of wool fibre morphogenesis,J.Structural Biol.,191(3):341-351
Rogers G.E.,2006,Biology of the wool follicle:an excursion into a unique tissue interaction system waiting to be rediscovered,Exp.Dermatol.,15(12):931-949
Saito A.,Sugawara A.,Uruno A.,Kudo M.,Kagechika H.,Sato Y.,Owada Y.,Kondo H.,Sato M.,Kurabayashi M.,Lmaizumi M.,Tsuchiya S.,and Ito S.,2007,All-trans retinoic acid induces in vitro angiogenesis via retinoic acid receptor:Possible involvement of paracrine effects of endogenous vascular endothelial growth factor signaling,Endocrinol.,148(3):1412-1423
Wagatsuma A.,2007,Endogenous expression of angiogenesis-related factors in response to muscle injury,Mol.Cell Biochem.,298(1):151-159
Wang J.,Dai Y.J.,Wang Y.,Zi X.D.,Ouyang X.,and Liu L.S.,2010,KAP 6.1 and KAP 6.2 loci and its relation to down traits of tibetan goats,Xinan Minzu Daxue Xuebao(Journal of Southwest University for Nationalities(Natural Science Edition)),36(6):962-966(王杰,代怡倩,王永,字向东,欧阳熙,刘鲁蜀,2010,高原型藏山羊KAP6.1和KAP6.2位点与产绒性状的关系研究,西南民族大学学报(自然科学版),36(6):962-966)
Wang Z.Y.,Chen Y.L.,Xu Q.L.,Ma Z.R.,and Qi Q.Q.,2011,Polymorphism of KAP3.2 gene and its effect on partial economic traits in Tibetan sheep,Xumu Shouyi Xuebao(Chinese Journal of Animal and Veterinary Sciences),42(2):284-288(王志有,陈玉林,徐秋良,马忠仁,祁全青,2011,藏系绵羊KAP3.2基因多态性及其对部分经济性状的影响,畜牧兽医学报,42(2):284-288)
Yang K.W.,Hu L.,Luo B.,Liu S.,Zheng Y.C.,and Zi X.D.,2014,Cloning and tissue expression of KAP3.2 gene in tibetan sheep,Xinan Minzu Daxue Xuebao(Journal of Southwest University for Nationalities(Natural Science Edition)),40(6):809-813(杨珂伟,胡亮,罗斌,刘霜,郑玉才,字向东,2014,藏绵羊KAP3.2基因的cDNA克隆,序列分析及组织表达的研究,西南民族大学学报(自然科学版),40(6):809-813)
Yang Z.Q.,Tao J.Z.,Li Y.K.,Ding W.,Liu Y.B.,and Wei B.D.,2017,Study on differentially expressed proteins of Tan sheep skin using i TRAQ technology,Zhongguo Xumu Shouyi(China Animal Husbandry&Veterinary Medicine),44(9):2682-2691(杨佐青,陶金忠,李颖康,丁伟,刘永斌,魏炳栋,2017,应用i TRAQ技术对滩羊裘皮差异表达蛋白的研究,中国畜牧兽医,44(9):2682-2691)
Yao J.Y.,Bao H.X.,Luan W.M.,and Jiang H.Z.,2010,Study on expression of VEGF gene in hair follicles of Liaoning cashmere goats,Zhongguo Xumu Shouyi(China Animal Husbandry&Veterinary Medicine),37(12):139-141(姚纪元,包红喜,栾维民,姜怀志,2010,辽宁绒山羊皮肤毛囊血管内皮生长因子基因的表达研究,中国畜牧兽医,37(12):139-141)
Yi J.,Zhu Y.,Jia Y.,Jiang H.D.,Zheng X.,Liu D.J.,Gao S.X.,Sun M.J.,Hu B.,Jiao B.H.,Wang L.H.,and Wang K.H.,2016,The annexin a2 promotes development in arthritis through neovascularization by amplification hedgehog pathway,PLo S One,11(3):e0150363
Zhao J.Y.,2013,Study on the growth-development and productivity of Tan sheep in Ningxia,Thesis for M.S.,Northwest A&F University,Supervisor:Luo J.,pp.1-6(赵金宇,2013,宁夏滩羊早期生长发育规律和生产性能研究,硕士学位论文,西北农林科技大学,导师:罗军,pp.1-6)
Zhao Z.C.,Liu G.B.,Li X.Y.,Huang J.,Xiao Y.J.,Du X.Y.,and Yu M.,2016,Characterization of the promoter regions of two sheep keratin associated protein genes for hair cortexspecific expression,PLoS One,11(4):e0153936
Zhao L.P.,and Lu X.Y.,2010,Expression of annexin A2 and Bcl-2in cervical cancer and its significance,Xuzhou Yixueyuan Xuebao(Acta Academiae Medicinae Xuzhou),30(8):507-510(赵丽萍,陆晓媛,2010,Annexin A2,Bcl-2在宫颈癌中的表达及意义,徐州医学院学报,30(8):507-510)
Zhou H.,Gong H.,Li S.,Luo Y.,and Hickford J.G.H.,2015,A57 bp deletion in the ovine KAP6-1 gene affects wool fibre diameter,Journal of Animal Breeding and Genetics,13(4):301-307
Zhou H.T.,Gong H.,Wang J.Q.,Dyer J.M.,Luo Y.Z.,and Hickford J.G.H.,2016,Identification of four new gene members of the KAP6 gene family in sheep,Sci.Rep.,6:24074
基本信息:
DOI：10.13417/j.gab.039.000018
中图分类号:S826
引用信息:
[1]陈永宏,杨佐青,陶金忠,等.应用iTRAQ技术分析滩羊二毛期不同花穗型裘皮差异蛋白[J].基因组学与应用生物学,2020,39(01):18-29.DOI:10.13417/j.gab.039.000018.
基金信息:
宁夏农业育种专项项目(2013NYYZ0402)资助
2020-01-25
2020-01-25