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运用ISSR标记对来自深圳、北海和防城港的三个细基江蓠繁枝变种群体进行遗传多样性检测,筛选出的16条ISSR引物共扩增出111条带,引物平均多态位点比例为54.95%。三个群体的多态位点比例深圳群体最高为23.42%,北海群体最低为4.50%;群体平均多态位点比例为11.12%,平均基因多样性(Hs)为0.051 3,表明细基江蓠繁枝变种群体遗传多样性较低。群体遗传分化指数为0.756 5,说明遗传变异主要存在于群体间且群体间的遗传分化水平较高。Mantel检验发现遗传距离与地理距离无关。适应生态环境选择无性繁殖模式可能是细基江蓠繁枝变种群体遗传多样性较低、群体间遗传分化大的重要因素。
Abstract：ISSR(inter-simple sequence repeats) markers were applied to study the genetic diversity of three Gracilaria tenuistipitata var Liui populations from Shenzhen,Beihai and Fangchengang.The sixteen selected ISSR primers amplified 111 bands with a average percentage of polymorphic loc of 54.95%.The percentage of polymorphic loc of three populations ranged from 23.42% to 4.50%,highest in the Shengzhen populations and lowest in the Beihai populations.The average polymorphic loc among populations was 11.12% and Hs was 0.051 3,that infers the genetic diversity of three G.tenuistipitata var Liui populations were low.The Nei's genetic differentiation index GST was 0.756 5,indicating that genetic variation mainly existed among populations and the level of genetic differentiation between populations was higher.The Mantel test showed that genetic distance was less correlated with geographic distance.Asexual reproduction might be one importance factor which cause low genetic diversity and high genetic differentiation among G.tenuistipitata var Liui populations.
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基本信息:
DOI：10.13417/j.gab.033.000121
中图分类号:S968.434
引用信息:
[1]戴晓玲,杨芳,介白飞,等.细基江蓠繁枝变种(Gracilaria tenuistipitata var.Liui)群体遗传多样性的ISSR分析[J].基因组学与应用生物学,2014,33(01):121-127.DOI:10.13417/j.gab.033.000121.
基金信息:
广西自然科学基金(2013GXNSFDA019012)资助
2014-02-28
2014-02-28