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通过检测慢性复合应激小鼠在Morris水迷宫实验中记忆能力及其杏仁体和海马神经元电生理特性的变化探讨情绪性记忆相关脑区的作用机制及神经元网络可塑性变化的机理。水迷宫实验中两组小鼠的逃避潜伏期逐渐缩短,具有显著性差异,6个训练周期中,应激组有5个训练周期的逃避潜伏期较对照组缩短,存在显著性差异;应激组小鼠在目标象限游泳停留时间显著长于其在其它3个象限的时间,同时显著长于对照组,而在对侧象限游泳时间显著短于对照组。小鼠在慢性负性刺激下学习和记忆能力得到提升,其杏仁体内GABA能神经元功能出现下降、海马锥体神经元功能出现增强,表明情绪性记忆可能与海马和杏仁体的神经元网络整体兴奋性的可塑性变化有关。
Abstract：The objective of this study is to investigate the mechanism underlying the brain regions associated with emotional memory and the plasticity of neural network by detecting the changes of memory ability in the Morris water maze test after chronic compound stress in mice and the electrophysiological characteristics of the neurons in amygdala and hippocampus. Morris water maze test showed that the escape latency of the two groups decreased significantly. During six training cycles, the escape latency of five training cycles in stress group was shorter than that in control group. The mice in the stress group stayed longer in the target quadrant than those in the other three quadrants, and significantly longer in the control group, while the swimming time in the opposite quadrant was significantly shorter than that in the control group. Chronic compound stress improves the ability of spatial learning and memory retention in mice, deteriorates the function of GABAergic neurons in amygdala and elevates the function of pyramidal neurons in hippocampus. It is suggested that emotional memory may be related to the plasticity of the global excitability of neurons in hippocampus and amygdala.
Abriel H.,2007,Roles and regulation of the cardiac sodium channel Nav1.5:Recent insights from experimental studies,Cardiovasc.Res.,76(3):381-389
Annecchino L.A.,Morris A.R.,Copeland C.S.,Agabi O.E.,Chadderton P.,and Schultz S.R.,2017,Robotic automation of in vivo two-photon targeted whole-cell patch-clamp electrophysiology,Neuron,95(5):1048-1055
Baczkowski B.M.,van Zutphen L.,Siep N.,Jacob G.A.,Domes G.,Maier S.,Sprenger A.,Senft A.,and Willenborg B.,2017,Deficientamygdala-prefrontal intrinsic connectivity after effortfulemotionregulation in borderline personality disorder,Eur.Arch.Psychiatry Clin.Neurosci.,267(6):551-565
Chen C.C.,Cang C.,Fenske S.,Butz E.,Chao Y.K.,Biel M.,Ren D.,Wahl-Schott C.,and Grimm C.,2017,Patch-clamptechnique to characterize ion channels in enlarged individual endolysosomes,Nat.Protoc.,12(8):1639-1658
Chen N.,Chen X.,Yu J.D.,and Wang J.H.,2006,Afterhyperpolarization improves spike programming through lowering threshold potentials and refractory periods mediated by voltage-gated sodium channels,Biochem.Bioph.Res.Co.,346(3):938-945
Emery A.A.,Heath N.L.,and Mills D.J.,2016,Basic psychological need satisfaction,emotion dysregulation,and non-suicidal self-Injury engagement in young adults:An application of self-determination theory,J.Youth Adolescence,45(3):612-623
Guan S.D.,Ma S.F.,Zhu Y.,and Wang J.H.,2006,The intrinsic mechanisms underlying the maturation of programming sequential spikes at cerebellar Purkinje cells,Biochem.Bioph.Res.Co.,345(1):175-180
Hoeijmakers L.,Amelianchik A.,Verhaag F.,Kotah J.,Lucassen P.J.,and Korosi A.,2018,Early-Life stress does not aggravate spatial memory or the process of hippocampal neurogenesis in adult and middle-aged APP/PS1 mice,Front.Aging.Neurosci.,21(4):235-249
Kark S.M.,Slotnick S.D.,and Kensinger E.A.,2016,Repetition enhancement of amygdalaand visual cortex functional connectivity reflects nonconscious memoryfor negative visual stimuli,J.Cogn.Neurosci.,28(12):1933-1946
Kiernan M.C.,Kirshnan A.V.,Lin C.S.,Burkke D.,and Berkovic S.F.,2005,Mutation in the Na+channel subunit SCN1B produces paradoxical changes in peripheral nerve excitability,Brain,128(8):1841-1846
Kragel P.A.,and LaBar K.S.,2014,Advancingemotion theorywith multivariate pattern classification,Emot.Rev.,6(2):160-174
Larkum M.E.,Waters J.,Sakmann B.,and Helmchen F.,2007,Dendritic spikes in apical dendrites of neocortical layer 2/3pyramidal neurons,Neurosci,27(34):8999-9008
Li H.,Zhong Z.H.,Yang J.,and Dong J.,2015,Research on hippocampal synaptic plasticity and traumatic stress disorder(PTSD),Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(3):658-662(李航,钟志鸿,杨进,董靖,2015,海马突触可塑性与创伤后应激障碍(PTSD)相关研究,基因组学与应用生物学,34(3):658-662)
Marie A.,Meunier J.,Brun E.,Malmstrom S.,Baudoux V.,Flaszka E.,Naert G.,Roman F.,Cosnier-Pucheu S.,and Gonzalez-Gonzalez S.,2018,N-acetylcysteine treatment reduces age-related hearing loss and memory impairment in the senescence-accelerated prone 8(SAMP8)mouse model,Aging.Dis.,9(4):664-673
Mathew R.S.,Mullan H.,Blusztajn J.K.,and Lehtinen M.K.,2016,Comment on"Multiple repressive mechanisms in the hippocampus during memory for mation",Science,353(6298):453
Molinarolo S.,Lee S.,Leisle L.,Lueck J.D.,Granata D.,Carnevale V.,and Ahern C.A.,2018,Cross-kingdom auxiliary subunit modulation of a voltage-gated sodium channel,J.Biol.Chem.,293(14):4981-4992
Mothersill O.,Knee-Zaska C.,and Donohoe G.,2016,Emotion and theory of mind in schizophrenia-investigating the role of the cerebellum,Cerebellum,15(3):357-368
Ohgidani M.,Kato T.A.,Sagata N.,Hayakawa K.,Shimokawa N.,Sato-Kasai M.,and Kanba S.,2016,TNF-αfrom hippocampal microglia induces working memory deficits byacutestressinmice,Brain.Behav.Immun.,55:17-24
Patel D.,Anilkumar S.,Chattarji S.,and Buwalda B.,2018,Repeated social stress leads to contrasting patterns of structural plasticity in the amygdala and hippocampus,Behav.Brain Res.,347:314-324
Piantadosi P.T.,Yeates D.C.M.,Wilkins M.,and Floresco S.B.,2017,Contributions of basolateralamygdalaand nucleus accumbens subregions to mediating motivational conflict during punished reward-seeking,Neurobiol.Learn.Mem.,140:92-105
Quas J.A.,Rush E.B.,Yim I.S.,Edelstein R.S.,Otgaar H.,and Smeets T.,2016,Stress andemotionalvalence effects on children's versus adolescents'true and falsememory,Memory,24(5):696-707
Reagh Z.M.,and Ranganath C.,2018,What does the functional organization of cortico-hippocampal networks tell us about the functional organization ofmemory?Neurosci.Lett.,680:69-76
Sebastian C.L.,McCrory E.J.,BritoD.S.,and Viding E.,2017,Modulation of amygdala response to task-irrelevant emotion,SocCogn Affect Neurosci,12(4):643-650
Tan Y.X.,and Jia Y.C.,2017,Differential expression and regulation of two critical periods of transcription and translation in hippocampus during memory formation,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(8):3187-3193(谭月茜,贾怡昌,2017,记忆形成时海马区两个转录翻译关键期的差异表达及调控,基因组学与应用生物学,36(8):3187-3193)
Voss J.L.,Bridge D.J.,Cohen N.J.,and Walker J.A.,2017,ACloser Look at the hippocampusand memory,Trends Cogn.Sci.,21(8):577-588
Wang J.H.,and Kelly P.T.,2001,Calcium-calmodulin signalingpathway up-regulates glutamatergic synaptic function innon-pyramidalfast spiking rat hippocampal CA1 neurons,Physiol.,533(2):407-422
Yu J.Y.,Fang P.,Wang C.,Wang X.X.,Li K.,Gong Q.,Luo B.Y.,and Wang X.D.,2018,Dorsal CA1 interneurons contribute toacutestress-induced spatial memory deficits,Neuropharmacol.,135:474-486
Zhang M.,Hung F.,Zhu Y.,Xie Z.,and Wang J.,2004,Calcium signal dependent plasticity of neuronal excitability developed postnatally,Neurobiol.,61(2):277-287
Zhou Y.,Ma C.,Li B.M.,and Sun C.,2018,Polygala japonica Houtt.reverses depression-like behavior and restores reduced hippocampal neurogenesis inchronic stressmice,Biomed.Pharmacother.,99:986-996
基本信息:
DOI：10.13417/j.gab.039.002394
中图分类号:R338
引用信息:
[1]刘冬播,石彦,陈传好,等.负性刺激诱导小鼠情绪性记忆脑区神经元编程的可塑性[J].基因组学与应用生物学,2020,39(05):2394-2403.DOI:10.13417/j.gab.039.002394.
基金信息:
蚌埠医学院自然科学基金项目(BYKY1652);蚌埠医学院科技发展基金项目(BYKF1754);; 蚌埠市级科技创新指导类项目(20160306)共同资助
2019-04-10
2019-04-10
2019-04-10