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通过基因芯片技术,利用Roche-NimbleGen公司制作的大鼠12×135K全基因组表达谱芯片,对日龄为6d和10d的大鼠睾丸组织进行全基因组表达差异分析。结果显示:具有2倍以上的差异表达基因有4298个,其中表达上调的基因共1878个,表达下调的基因共2420个。这些差异表达的基因中有3154个基因具有基因本体注释,参与了154个生物学通路。进一步分析表明具有8倍以上差异表达的基因有13个,这些基因参与了生物学过程、细胞组分和分子功能等基因本体分类,进一步选择3个差异表达的基因,LOC686076、Cxcl6和Trib3,做了实时定量RT-PCR检测。其结果趋势与芯片数据一致。因此,我们初步认为精原干细胞的发生与增殖在大鼠早期的发育过程中已经有大量的基因参与,是一个多基因协调表达的过程。
Abstract：In this research,differential analysis of genome-wide expression of the rat testis tissues in the age of 6 and 10 days was conducted by gene chip technology based on the 12×135K genome-wide EST microarray of rat produced by Roche-NimbleGen.The results showed that there were 4 298 genes with 2-fold or more differentially expressed in the rat testis tissues between the ages of 6 and 10 days,thereinto,1 878 genes were up-regulated expression and 2 420 genes were down-regulated expression.3 154 genes of these differentially expressed genes have been annotated with gene ontology,participating in the 154 biological pathways.Further analysis showed that there were 13 genes with more than 8 fold differential expression,those genes involved in gene ontology categories,such as biological processes,cellular components and molecular functions and so on.Three differentially expressed genes,LOC686076,Cxcl6 and Trib3,were chosen to detect by real-time quantitative RT-PCR,the results exhibited a tread toward the microarray data.Therefore,we preliminary thought that a large number of genes should be involved in the occurrence and proliferation of spermatogonial stem cell in early stages of growth and development of the rat,it might be a synchronizing process of multi-gene expression.
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基本信息:
中图分类号:Q78
引用信息:
[1]刘陶迪,罗奋华,于泊洋,等.6天龄与10天龄大鼠的睾丸组织的基因差异表达[J].基因组学与应用生物学,2012,31(01):40-44.
基金信息:
教育部“春晖计划”合作科研项目(Z2007-1-01036);; 内蒙古自然科学基金项目(2009ZD05)共同资助
2012-02-28
2012-02-28