| 1,671 | 40 | 100 |
| 下载次数 | 被引频次 | 阅读次数 |
病毒诱导的基因沉默(virus induced gene silencing,VIGS)是指携带目标基因片段的重组载体在侵染植株后,可诱导植物内源基因发生沉默。该技术已经广泛用于植物功能基因研究。本文针对VIGS技术分子机理、载体来源、载体选择标记、技术局限性以及其在植物基因功能研究中的应用做一概述,展望了VIGS技术作为植物功能研究的前景和需要改进的方向。
Abstract：Virus-induced gene silencing(VIGS) is a new technology which has been widely used in functional analysis for plants. The recombinant virus vector which has target gene of plant can induce plant endogenous gene silence. The technology has been widely used in the research of plant gene function. In this paper, advance about molecular mechanism, vector source and selected marker, limitations of VIGS, and its application in functional research about plant genes, was reviewed. And the prospects of VIGS in plant functional genes and the direction of progressing are introduced.
Angell S.M.,and Baulcombe D.C.,1997,Consistent gene silencing in transgenic plants expressing a replicating potato virusX RNA,EMBO J.,16(12):3675-3684
Bachan S.,and Dinesh-Kumar S.P.,2012,Tobacco rattle virus(TRV)-based virus-induced gene silencing,Methods inMolecular Biology(Clifton,N.J.),894:83-92
Burch-Smith T.M.,Anderson J.C.,Martin G.B.,and Dinesh-Kumar S.P.,2004,Applications and advantages of virus-induced gene silencing for gene function studies in plants,ThePlant Journal,39(5):734-746
Burch-Smith T.M.,Schiff M.,Liu Y.,and Dinesh-Kumar S.P.,2006,Efficient virus-induced gene silencing in Arabidopsis,Plant Physiol.,142(1):21-27
Cai X.,Wang C.,Xu Y.,Xu Q.,Zheng Z.,and Zhou X.,2007,Efficient gene silencing induction in tomato by a viral satellite DNA vector,Virus Research,125(2):169-175
Chen J.C.,Jiang C.Z.,and Reid M.S.,2005,Silencing a prohibitin alters plant development and senescence,Plant J.,44(1):16-24
Chen J.C.,Jiang C.Z.,Gookin T.E.,Hunter D.A.,Clark D.G.,and Reid M.S.,2004,Chalcone synthase as a reporter invirus-induced gene silencing studies of flower senescence,Plant Molecular Biology,55(4):521-530
Fofana I.B.,Sangare A.,Collier R.,Taylor C.,and Fauquet C.M.,2004,A geminivirus-induced gene silencing system for genefunction validation in cassava,Plant Molecular Biology,56(4):613-624
Gossele V.,Fache I.,Meulewaeter F.,Cornelissen M.,and Metzlaff M.,2002,SVISS-a novel transient gene silencing system for gene function discovery and validation in tobaccoplants,The Plant Journal,32(5):859-866
Guo Y.,Huang C.,Xie Y.,Song F.,and Zhou X.,2010,A tomatoglutaredoxin gene Sl GRX1 regulates plant responses to oxidative,drought and salt stresses,Planta,232(6):1499-1509
Ho F.I.,Chen Y.Y.,Lin Y.M.,Cheng C.P.,and Wang J.F.,2009,A tobacco rattle virus-induced gene silencing system for asoil-borne vascular pathogen Ralstonia solanacearum,Botanical Studies,50:413-424
Kjemtrup S.,Sampson K.S.,Peele C.G.,Nguyen L.V.,ConklingM.A.,Thompson W.F.,and Robertson D.,1998,Gene silencing from plant DNA carried by a geminivirus,The PlantJournal,14(1):91-100
Kramer E.M.,Holappa L.,Gould B.,Jaramillo M.A.,Setnikov D.,and Santiago P.M.,2007,Elaboration of B gene function toinclude the identity of novel floral organs in the lower eudicot Aquilegia,Plant Cell,19(3):750-766
Kumagai M.H.,Donson J.,della-Cioppa G.,Harvey D.,HanleyK.,and Grill L.K.,1995,Cytoplasmic inhibition ofcarotenoid biosynthesis with virus-derived RNA,Proc.Natl.Acad.Sci.USA,92(5):1679-1683
Liu Y.L.,Schiff M.,and Dinesh-Kumar S.P.,2002,Virus-inducedgene silencing in tomato,Plant Journal,31(6):777-786
Maimbo M.,Ohnishi K.,Hikichi Y.,Yoshioka H.,and Kiba A.,2010,S-glycoprotein-like protein regulates defense responses in Nicotiana plants against Ralstonia solanacearum,PlantPhysiol.,152(4):2023-2035
Orzaez D.,Medina A.,Torre S.,Fernandez-Moreno J.P.,RamblaJ.L.,Fernandez-Del-Carmen A.,Butelli E.,Martin C.,andGranell A.,2009,A visual reporter system for virus-inducedgene silencing in tomato fruit based on anthocyanin accumulation,Plant Physiol.,150(3):1122-1134
Ratcliff F.,Martin-Hernandez A.M.,and Baulcombe D.C.,2001,Tobacco rattle virus as a vector for analysis of gene functionby silencing,Plant Journal,25(2):237-245
Re D.A.,Dezar C.A.,Chan R.L.,Baldwin I.T.,and BonaventureG.,2011,Nicotiana attenuata Na HD20 plays a role in leafABA accumulation during water stress,benzylacetone emission from flowers,and the timing of bolting and flower transitions,J.Exp.Bot.,62:155-166
Scofield S.R.,Huang L.,Brandt A.S.,and Gill B.S.,2005,Development of a virus-induced gene-silencing system forhexaploid wheat and its use in functional analysis of theLr21-mediated leaf rust resistance pathway,Plant Physiology,138(4):2165-2173
Turnage M.A.,Muangsan N.,Peele C.G.,and Robertson D.,2002,Geminivirus-based vectors for gene silencing in Arabidopsis,The Plant Journal,30(1):107-114
Voinnet O.,2001,RNA silencing as a plant immune system against viruses,Trends in Genetics,17:449-459
Wang C.C.,Cai X.Z.,Wang X.M.,and Zheng Z.,2006,Optimisation of tobacco rattle virus-induced gene silencing in Arabidopsis,Functional Plant Biology,33:347-355
Wang X.,Cao A.,Yu C.,Wang D.,Wang X.,and Chen P.,2010,Establishment of an effective virus induced gene silencingsystem with BSMV in Haynaldia villosa,Molecular BiologyReports,37(2):967-972
Wu C.,Jia L.,and Goggin F.,2011,The reliability of virus-induced gene silencing experiments using tobacco rattle virusin tomato is influenced by the size of the vector control,Molecular Plant Pathology,12(3):299-305
Yamanouchi U.,Yano M.,Lin H.,Ashikari M.,and Yamada K.,2002,A rice spotted leaf gene,Spl7,encodes a heat stresstranscription factor protein,Proc.Natl.Acad.Sci.USA,99(11):7530-7535
Zheng S.J.,Snoeren T.A.,Hogewoning S.W.,van Loon J.J.,andDicke M.,2010,Disruption of plant carotenoid biosynthesisthrough virus-induced gene silencing affects oviposition behaviour of the butterfly Pieris rapae,New Phytologist,186(3):733-745
基本信息:
DOI：10.13417/j.gab.033.001401
中图分类号:Q943
引用信息:
[1]李小龙,谢伶俐,焦靖芝,等.VIGS技术在基因功能研究中的应用进展[J].基因组学与应用生物学,2014,33(06):1401-1404.DOI:10.13417/j.gab.033.001401.
基金信息:
国家863计划项目(2011AA10A104)资助
2014-12-28
2014-12-28