| 407 | 33 | 146 |
| 下载次数 | 被引频次 | 阅读次数 |
本研究以去卵巢骨质疏松症模型大鼠为研究对象,采用比较分析与去卵巢骨质疏松症相关的多项因子的方法,试图探究左、右归丸的效应机理。将雌性SD大鼠随机分为空白组、假手术组、模型组(OVX组)、己烯雌酚组、左归丸组、右归丸组,每组12只。除空白组、假手术组外,将各组大鼠双侧卵巢切除制备绝经后骨质疏松症大鼠模型。造模一周后,分别给予各组相应的蒸馏水或药物,持续治疗12周。测定各组大鼠腰椎骨密度、血清钙、血清碱性磷酸酶(ALP)含量和大鼠胫骨骨髓中OPG、RANKL、Wnt1蛋白的表达量。与空白组、假手术组相比,模型组大鼠OPG、骨密度均显著下降(p<0.01),而ALP、RANKL、Wnt1明显升高(p<0.01);各给药组较OVX组,OPG、骨密度不同程度提高,ALP、RANKL、Wnt1则有不同程度降低(p<0.05)。结果表明,左、右归丸能够抑制骨吸收,降低骨转化率,使骨形成和骨吸收趋于平衡,可有效防治去卵巢骨质疏松症模型大鼠的骨流失并改善骨组织形态,并且右归丸疗效优于左归丸;本研究为中医防治PMOP提供坚实的实验依据,并从分子水平揭示左、右归丸的治疗机理,推动了左、右归丸治疗PMOP作用机制的研究。
Abstract：This study took the postmenopausal osteoporosis rats model as the research object to explore the mechanism of Zuogui pill and Yougui pill by the method of comparative analysis and multinomial factors which is relevant to the osteoporosis of ovaries-removed. Healthy female rats were randomly divided into blank control group, sham operation group, ovariectomized model group, diethylstilbestrol group, Zuogui pill group and Yougui pill group, with 12 rats in each group. Ovariectomy was performed to create the rat osteoporosis model except rats in the sham operation group and blank control group. After the models were established for 1 week,diethylstilbestrol group, Zuogui pill group, Yougui pill group were given corresponding distilled water or drugs respectively, and the treatment persisted for 12 weeks. The rats of every group were tested BMD of the lumvar vertebrae, serum Ca content, Serum alkaline phosphatase(ALP) content and the protein expression of OPG,RANKL, Wnt1 of tibia bone marrow. Compared with the blank control group and sham operation group, BMD and OPG expression of rats in model group significantly decreased(p<0.01), while ALP and the expression of RANKL and Wnt1 increased(p<0.01). Compared with the model group, diethylstilbestrol group, Zuogui pill group and Yougui pill group could significantly increase expression of BMD and OPG, reduction of ALP,expression of RANKL and Wnt1(p<0.05). The result showed that Zuogui pill and Yougui pill could inhibit bone resorption and reduce bone turnover rates in a certain extent. It could make the bone formation and bone resorption into balance, which could prevent the bone loss of ovariectomized rats model of osteoporosis, and improve bone tissue form. In addition, Zuogui pill was more effective. This study might provide the experimental bases to cure PMOP using traditional Chinese medicine and reveal the therapeutic mechanism of Zuogui pill and Yougui pill from the molecular level, promoting the mechanism research of curing PMOP using Zuogui pill and Yougui pill.
Boumaza I.,Srinivasan S.,Witt W.T.,Feghali-Bostwick C.,Dai Y.,Garcia-Ocana A.,and Feili-Hariri M.,2009,Autologous bone marrow-derived rat mesenchymal stem cells promote PDX-1 and insulin expression in the islets,alter T cell cytokine pattern and preserve regulatory T cells in the periphery and induce sustained normoglycemia,Journal of Autoimmunity,32(1):33-42
Fu X.W.,Song C.H.,Zhang Z.G.,Yu Z.,Liu H.,Pang J.H.,Li Y.,Wang S.J.,Wang W.L.,Li Y.Z.,Wu J.Y.,Zhao H.Y.,Liu M.J.,and Ju D.H.,2016,Discuss the gender differences of kidney advocate bone and its mechanism from the OPG-RANKL osteoclast regulation pathways and Wnt osteoblast regulation pathways,Zhongguo Zhongyi Jichu Yixue Zazhi(Chinese Journal of Basic Medicine in Traditional Chinese Medicine),22(1):66-72(付小卫,宋长恒,张治国,于峥,刘红,潘静华,李艳,王少君,汪文来,李岳泽,吴佳莹,赵宏艳,刘梅洁,鞠大宏,2016,从OPG-RANKL破骨细胞调控通路和Wnt成骨细胞调控通路探讨“肾主骨”的性别差异及其机制,中国中医基础医学杂志,22(1):66-72)
Huang W.,Yang S.,Shao J.,and Li Y.P.,2007,Signaling and transcriptional regulation in osteoblast commitment and differentiation,Front.Biosci.,12(8):3068-3092
Kong X.H.,Niu Y.B.,Li Y.H.,and Mei Q.B.,2011,Progress of research on the relationship between OPG/RANK/RANKLsystem and Osteoporosis,Shengming Kexue Yanjiu(Life Science Research),15(1):80-85(孔祥鹤,牛银波,李宇华,梅其炳,2011,OPG/RANK/RANKL系统与骨质疏松研究最新进展,生命科学研究,15(1):80-85)
Lewiecki E.M.,2011,New targets for intervention in the treatment of postmenopausal osteoporosis,Nature Review Rheumatology,7(11):631-638
Liu H.,Li L.F.,Shi W.M.,Yang J.Q.,and Wu L.H.,2016,Progress on the role of estrogen receptor in bone formation and metabolism,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(7):1656-1611(刘海,李林福,施伟梅,杨建琼,吴龙火,2016,雌激素受体在骨形成代谢中的研究进展,基因组学与应用生物学,35(7):1656-1611)
Li X.J.,Harold M.F.,Zhu S.S.,and Ke H.Z.,2001,The new con cept of bone biology,Zhongguo Guzhi Shusong Zazhi(Chinese Journal of Osteoporosis),7(2):152-174(黎小坚,Harold M Frost,朱绍舜,柯华珠,2001,基础骨生物学新观,中国骨质疏松杂志,7(2):152-174)
Nusse R.,2001,Making head or tail of Dickkopf,Nature,411(6835):255-256
O'Neill S.,Mac Lennan A.,Diamond T.,Ebeling P.,Findlay D.,Flicker L.,Markwell A.,Nowson C.,Pocock N.,Sambrook P.,and Fiatarone-Singh M.,2004,Guidelines for the management of postmenopausal osteoporosis for GPs,Aust.Fam.Physician.,33(11):911-917
Pritchett J.W.,2001,Statin therapy decreases the risk of osteonecrosis in patients receiving steroids,Clinical Orthopaedics and Related Research,386:173-178
Schoppet M.,Preissner K.T.,and Hofbauer L.C.,2002,RANKligand and osteoprotegerin:paracrine regulators of bone metabolismand vascular function,Arteriosclerosis,thrombosis,and vascular biology,Arterioscler.Thromb.Vasc.Biol.,22(4):549-553
Sharma S.M.,Hu R.,Bronisz A.,Meadows N.,Lusby T.,Fletcher B.,Hume D.A.,Cassady A.I.,and Ostrowski M.C.,2006,Genetics and genomics of osteoclast differentiation integrating cell signaling pathways and gene networks,Crit.Rev.Eukaryot.Gene Expr.,16(3):253-277
Sun Y.J.,Ren Y.L.,Qu N.N.,Meng Y.,and Li S.J.,2015,The effect of Zuogui pill,Yougui pill,and their disassembled prescriptions on ovariectomized osteoporotic rats,Zhongguo Guzhi Shusong Zazhi(Chinese Journal of Osteoporosis),21(8):910-913(孙月娇,任艳玲,曲宁宁,孟玥,李书娟,2015,左、右归丸及其拆方对去卵巢骨质疏松模型大鼠的比较研究,中国骨质疏松杂志,21(8):910-913)
Yi W.L.,Liao D.Q.,Lin B.Y.,Xiao Y.J.,and Yuan H.Y.,2012,The changes and rela tionship with sex hormone,cytokine and bone metabolic index in postmenopausal osteoporosis patients,Jianyan Yixue(Laboratory Medicine),27(4):296-298(易伟莲,廖德权,林柏云,肖亚景,袁汉尧,2012,绝经后骨质疏松症患者性激素细胞因子及骨代谢指标的变化及关系,检验医学,27(4):296-298)
Yu Z.F.,Gu S.Z.,Gao J.J.,Zhou Y.,and Jin W.F.,2004,Changes of serum alkaline phosphatase levels and expressions of osteogenesis-related genes in bone marrow cells of ovariecfomized rats,Zhongguo Guzhi Shusong Zazhi(Chinese Journal of Osteoporosis),10(2):152-154(于志峰,顾淑珠,高建军,周轶,金慰芳,2004,去势大鼠血清ALP动态及骨髓细胞骨形成相关基因表达研究,中国骨质疏松杂志,10(2):152-154)
Zheng L.R.,and Huang H.J.,2012,The research of Zuogui pill for the treatment of osteoporosis,Zhongguo Guzhi Shusong Zazhi(Chinese Journal of Osteoporosis),18(6):576-578(郑鲤榕,黄惠娟,2012,左归丸治疗绝经后骨质疏松症研究概况,中国骨质疏松杂志,18(6):576-578)
Zhang H.,Chen G.F.,Wu J.Z.,and Chen Y.X.,2008,Significance of detecing Leptin/NO/IL-6 BGP/E2 in serum of Postmenopausal osteoporosis,Zhongguo Zhongyi Gushake Zazhi(Chinese Journal of Traditional Medical Traumatology and Orthopedics),16(2):1-3(张华,陈敢峰,吴俊哲,陈燕娴,2008,绝经后骨质疏松症血清中Leptin、NO、IL-6BGP及E2的检测意义,中国中医骨伤科杂志,16(2):1-3)
基本信息:
DOI：10.13417/j.gab.037.001104
中图分类号:R285.5;R-332
引用信息:
[1]王如然,冯艳华,张玉杰,等.左归丸、右归丸对去卵巢骨质疏松模型大鼠的效用[J].基因组学与应用生物学,2018,37(03):1104-1109.DOI:10.13417/j.gab.037.001104.
基金信息:
北京市中医药科技项目(课题号:JJ2014-13)资助
2018-03-25
2018-03-25