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极长链脂肪酸延伸酶蛋白家族(elongation of very-long-chain fatty acids, ELOVLs)是合成脂肪酸过程中的重要限速酶,主要调控血糖、血脂及一些代谢疾病的发生。为探究宁夏地方品种静原鸡ELOVL5基因编码蛋白的结构与功能,本试验根据GenBank中已公布的原鸡ELOVL5基因序列,针对8个外显子区域设计特异性引物,经DNA混合池扩增、测序后,对其编码区进行功能生物信息学分析。结果表明,静原鸡ELOVL5基因编码区全长1 023 bp,编码340个氨基酸;ELOVL5蛋白存在1个低复杂度保守序列及7个跨膜结构域,25个磷酸化位点,9个B细胞抗原表位,与acidPPc、BC10、RasGEFN蛋白存在部分重叠区域(41～142 aa,72～125 aa, 207～336 aa),为非分泌型的稳定水溶性蛋白,空间结构以α-螺旋和无规则卷曲为主;亚细胞定位结果显示ELOVL5蛋白可能在线粒体内行使重要的生物学功能。该研究结果为后续ELOVL5蛋白功能的深入研究提供科学依据。
Abstract：The family of extremely long-chain fatty acid extension enzymes(elong-chain fatty acids) is an important speed limit enzyme in the process of synthesizing fatty acids,which regulates the occurrence of blood sugar,lipids and some metabolic diseases.In order to explore the structure and function of the gene coding protein of Jingyuan chicken ELOVL5 of Ningxia local species,this experiment designed specific foreign primers for 8 exon regions according to the gene sequence of the original chicken ELOVL5 published in GenBank,and carried out functional bioinformatics analysis of the coding area after amplification and sequencing of the DNA mixing pool.The results showed that the full-length was 1 023 bp of ELOVL5 CDS sequence,and encoding 340 amino acids;ELOVL5 protein has 1 low complexity conservative sequence and 7 transmembrane domains,25 phosphorylation sites,9 B-cell antigen epitopes,partial overlap with acidPPc,BC10,RasGEFN protein(41 to 142 aa,72 to 125 aa,207 to 336 aa),a non-secretory stable water-soluble protein,the spatial structure dominated by Alpha-helix and random curl;Subcellular positioning results show that the ELOVL5 protein may perform important biological functions in the mitochondria.The results provide a scientific basis for the further in-depth study of ELOVL5 protein function.
Gregorio E.D.,Borroni B.,Giorgio E.,Lacerenza D.,Ferrero M.,Buono N.L.,Ragusa N.,Mancini C.,Gaussen M.,Calcia A.,Mitro N.,Hoxha E.,Mura I.,Coviello D.A.,Moon Y.A.,Tesson C.,Vaula G.,Couarch P.,Orsi L.,Duregon E.,Papotti M.G.,Deleuze J.F.,Imbert J.,Costanzi C.,Padovani A.,Giunti P.,Maillet-Vioud M.,Durr A.,Brice A.,Tempia F.,Funaro A.,Boccone L.,Caruso D.,Stevanin G.,and Brusco A.,2014,ELOVL5 mutations cause spinocerebellar ataxia 38,Am.J.Hum.Genet.,95(2):209-217
Guo P.C.,Dai S.S.,Yang H.R.,Zhao Z.H.,and Yan S.Q.,2017,ELOVL5 gene variants reveal significant effects of milk quality in holstein cows,Zhongguo Shouyi Xuebao (Chinese Journal of Veterinary Science),37(4):741-745 (郭鹏程,戴珊珊,杨浩然,赵志辉,闫守庆,2017,ELOVL5基因遗传变异与中国荷斯坦奶牛乳质性状的关联分析及功能验证,中国兽医学报,37(4):741-745)
Matsumoto H.,Shimizu Y.,Tanaka A.,Nogi T.,Tabuchi I.,Oyama K.,Taniguchi M.,Mannen H.,and Sasazaki S.,2013,The SNP in the promoter region of the bovine ELOVL5 gene influences economic traits including subcutaneous fat thickness,Mol.Biol.Rep.,40(4):3231-3237
Hwang J.Y.,Lee H.J.,Go M.J.,Jang H.B.,Choi N.H.,Bae J.B.,Castillo-Fernandez J.E.,Bell J.T.,Spector T.D.,Lee H.J.,and Kim B.J.,2018,Genome-wide methylation analysis i dentifies ELOVL5 as anepigenetic biomarker for the risk of type2 diabetes mellitus,Sci.Rep.,8(1):14862
Lee M.C.,Park J.C.,Yoon D.S.,Choi H.,Kim H.J.,Shin K.H.,Hagiwara A,Han J.,Park H.G.,and Lee J.S.,2019,Ge nome-wide characterization and expression of the elongation of very long chain fatty acid (ELOVL) genes and fatty acid profiles in the alga (Tetraselmis suecica) fed marine rotifer Brachionus koreanus,Comp.Biochem.Physiol.Part D Genomics Proteomics,30:179-185
Lin Z.D.,Huang Y.S.,Zou W.G.,Rong H.,Hao M.L.,and Wen X.B.,2018,Cloning,tissue distribution,functional characterization and nutritional regulation of a fatty acyl ELOVL5elongase in chu's croaker Nibea coibor,Gene,659:11-21
Minghetti M.,Leaver M.J.,and Tocher D.R.,2011,Transcriptional control mechanisms of genes of lipid and fatty acid metabolism in the atlantic salmon (Salmo salar L.) established cell line,SHK-1,Biochim.Biophys.Acta,1811(3):194-202
Mu T.,Gu Y.L.,Zhao P.,Feng X.F.,Chen J.P.,Chen Y.F.,Liu L.Y.,Jiang Q.F.,and Zhang J.,2018,Study on polymorphism of ELOVL2 gene in Jingyuan chicken,Zhongguo Xumu Shouyi(China Animal Husbandry and Veterinary Medicine),45(3):730-737 (母童,顾亚玲,赵平,冯小芳,陈佳萍,陈亚飞,刘丽元,蒋秋斐,张娟,2018,静原鸡ELOVL2基因遗传多态性研究,中国畜牧兽医,45(3):730-737)
Mu T.,Zhang J.,Zhao P.,Gu Y.L.,Liu L.Y.,Yang Y.J.,An K.L.,and Wang Y.,2017,Tissue-specific expression analysis of ELOVL2 and ELOVL5 genes in Jingyuan chicken,Zhejiang Nongye Xuebao (Acta Agriculturae Zhejiangensis),29(8):1290-1296 (母童,张娟,赵平,顾亚玲,刘丽元,杨彦军,安克龙,王有,2017,静原鸡ELOVL2和ELOVL5基因表达的组织特异性研究,浙江农业学报,29(8):1290-1296)
Naganuma T.,Sato Y.,Sassa T.,Ohno Y.,and Kihara A.,2011,Biochemical characterization of the very long-chain fatty acid elongase ELOVL7,FEBS Lett.,585(20):3337-3341
Qin Y.,Dalen K.T.,Gustafsson J.A.,and Nebb H.I.,2009,Regulation of hepatic fatty acid elongase 5 by LXRα-SREBP-1c,Biochim.Biophys.Acta,1791(2):140-147
Shikama A.,Shinozaki H.,Takeuchi Y.,Matsuzaka T.,Aita Y.,Murayama T.,Sawada Y.,Piao X.Y.,Toya N.,Oya Y.,Takarada A.,Masuda Y.,Nishi M,Kubota M.,Izumida Y.,Nakagawa Y.,Iwasaki H.,Kobayashi K.,Yatoh S.,Suzuki H.,Yagyu H.,Kawakami Y.,Yamada N.,Shimano H.,and Yahagi N.,2015,Identification of human ELOVL5 enhancer re gions controlled by SREBP,Biochem.Biophys.Res.Commun.,465(4):857-863
Sun X.X.,2017,Characterization and function analysis of Fads2desaturase and ELOVL5 elongase gene in Trach-inotus ovatus,Thesis for M.S.,Shanghai Ocean University,Supervisor:Zhang D.C.,pp.33-34 (孙潇潇,2017,卵形鲳鲹Fads2去饱和酶与ELOVL5延长酶基因特征与功能研究,硕士学位论文,上海海洋大学,导师:张殿昌,pp.33-34)
Wang Y.,Moises T.G.,Sasmita T.,Daniela B.,Barbara C.,and Jump D.B.,2008,Elevated hepatic fatty acid elongase-5 activity affects multiple pathways controlling hepatic lipid and carbohydrate composition,J.Lipid Res.,49(7):1538-1552
Wu F.R.,Chen D.Y.,Jiang S.L.,and Li W.Y.,2017,Application of PBL teaching method in the bioinformatics teaching practice for the master's degree students,Fuyang Shifan Xueyuan Xuebao (Journal of Fuyang Teachers College (Natural Science Edition)),34(2):109-111 (吴风瑞,陈德宇,姜双林,李文雍,2017,PBL教学法在硕士研究生生物信息学教学中的应用,阜阳师范学院学报(自然科学版),34(2):109-111)
Moon Y.,Hammer R.E.,and Horton J.D.,2009,Deletion of ELO-VL5 leads to fatty liver through activation of SREBP-1c in mice,J.Lipid Res.,50(3):412-423
Zhang J.,Mu T.,Zhao P.,Chen J.P.,Feng X.F.,Guo P.,Wu Z.W.,Liu L.Y.,Jiang Q.F.,and Gu Y.L.,2019,Polymorphism of ELOVL5 gene in Jingyuan chicken,Zhejiang Nongye Xuebao (Acta Agriculturae Zhejiangensis),31 (2):200-206 (张娟,母童,赵平,陈佳萍,冯小芳,郭鹏,武泽文,刘丽元,蒋秋斐,顾亚玲,2019,静原鸡ELOVL5基因遗传多样性研究,浙江农业学报,31(2):200-206)
Zhang P.F.,Pu L.P.,Wang H.J.,Zhao X.L.,Chen F.M.,Lu Y.Q.,and Zhang M.,2017,The application of phosphoproteomics in mammalian sperm research,Jiyinzuxue yu Yingyong Shengwuxue (Genomics and Applied Biology),36(7):2862-2866 (张鹏飞,濮黎萍,王焕景,赵秀玲,陈富美,陆阳清,张明,2017,磷酸化蛋白质组学在哺乳动物精子研究中的应用,基因组学与应用生物学,36(7):2862-2866)
Zhao X.,Zhang F.B.,Wang H.Y.,Yan F.,An M.T.,Li Y.J.,Pang N.N.,and Ding J.B.,2019,Dominant T cell and B cell epitopes in Eg A31 protein of Echinococcus granulosus by bioinformatics,Zhongguo Zuzhi Gongcheng Yanjiu (Chinese Journal of Tissue Engineering Research),23(7):1078-1083(赵骁,张峰波,王红英,闫芳,安梦婷,李玉娇,庞楠楠,丁剑冰,2019,生物信息分析细粒棘球绦虫Eg A31蛋白T细胞及B细胞的优势抗原表位,中国组织工程研究,23(7):1078-1083)
Zhu L.C.,and Li J.R.,2017,Innovative teaching pattern of bioinformatics,Anhui Nongxue Tongbao (Anhui Agricultural Science Bulletin),23(6):179-181 (朱柳村,李嘉睿,2017,生物信息学创新教学模式的探究,安徽农学通报,23(6):179-181)
基本信息:
DOI：10.13417/j.gab.039.005432
中图分类号:S831.2
引用信息:
[1]张娟,母童,虎红红,等.静原鸡ELOVL5基因功能生物信息学分析[J].基因组学与应用生物学,2020,39(12):5432-5441.DOI:10.13417/j.gab.039.005432.
基金信息:
宁夏自然科学基金项目(2019AAC03070)资助
2020-12-25
2020-12-25