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建立测定发酵液中L-天冬氨酸的一种紫外分光光度测定方法,研究金属离子、反应时间、反应温度等对测定的影响,确定测定的最适条件,L-天冬氨酸溶液与茚三酮显色剂反应显蓝紫色,用紫外分光光度计对L-天冬氨酸反应溶液进行测定,得到最大吸收峰,根据最大吸收峰所对应的波长确定L-天冬氨酸吸光度与浓度间关系曲线。研究表明,L-天冬氨酸最佳吸收波长为510 nm,吸光度与浓度间工作曲线为:Y=0.067 8 X+0.013 5,r2=0.999 5,反应时间和反应温度对检测无影响,其他金属离子均不干扰L-天冬氨酸的测定,紫外分光光度法测定发酵液中L-天冬氨酸的操作简便、快捷、准确。
Abstract：An ultraviolet spectrophotometric detection method for L-aspartic acid content of the fermentation broth was established. The study of metal ions, reaction time and reaction temperature on the measurement of L-aspartic acid was researched. L-aspartic acid solution was mixed with ninhydrin, and reagent reaction was blue-violet. L-aspartic acid was determined by ultraviolet spectrophotometry. The maximum absorption wavelength of L-aspartic was 510 nm, The standard curve is: Y=0.067 8 X+0.013 5, r2=0.999 5. The reaction time and reaction temperature have little effect on detection, and other metal ions did not interfere with the determination of L-aspartate. The spectrophotometric method is simple rapid and accurate to determine the L-aspartate in fermentation broth.
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基本信息:
DOI：10.13417/j.gab.039.001692
中图分类号:O657.32;TQ922
引用信息:
[1]徐礼生,孙玥,柴存宝,等.紫外分光光度法测定发酵液中L-天冬氨酸[J].基因组学与应用生物学,2020,39(04):1692-1697.DOI:10.13417/j.gab.039.001692.
基金信息:
宿州市科技计划项目(SZ2017GG18);; 安徽省高校自然科学研究重点项目(KJ2017A440);; 宿州学院学术带头人项目(2018XJXS02)共同资助
2020-04-25
2020-04-25