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甘蔗镰孢菌(Fusarium sacchari)引起的甘蔗梢腐病严重影响了甘蔗的产量和质量。编码β-酮酯酰-辅酶A还原酶(β-ketoacyl-CoA reductase, KCR)的基因与脂肪酸合成有关，并为生物体提供超长链脂肪酸。本文对甘蔗镰孢菌KCR的相关基因FsKCR1侵染阶段的表达模式进行了分析，利用同源重组与基因互补的方法获得敲除突变体和互补菌株，同时对上述菌株的主要生物学特性进行研究。与野生型菌株FF001和互补菌株Com-△FsKCR1相比，敲除突变体△FsKCR1的生长速率、小型分生孢子产量和萌发速率以及致病力均显著降低。此外，缺失FsKCR1导致菌丝体疏水性下降。脂肪酸组分分析表明，FsKCR1参与调控甘蔗镰孢菌超长脂肪酸的合成。本研究结果为深入解析甘蔗镰孢菌致病机制提供重要依据。
Abstract：Pokkah boeng disease(PBD) caused by Fusarium sacchari seriously affects the yield and quality of sugarcane. The gene encoding β-ketoacyl-CoA reductase(KCR) is implicated in fatty acid synthesis and provides very long chain fatty acids for organisms. In this study, we analyzed the expression pattern of the KCR gene FsKCR1 in the infestation stage of F. sacchari. Gene deletion mutants and complementation strains were obtained using homologous recombination and gene complementation methods. Simultaneously, research was conducted on the primary biological characteristics of the aforementioned fungal strains. Compared with the wild-type strain FF001 and the complementation strain Com-△FsKCR1, the growth rate, production of microconidium, germination rate, and pathogenicity of the deletion mutant △FsKCR1 were significantly reduced. In addition, the hyphae of the mutant showed reduced hydrophobicity. Fatty acid composition analysis revealed that FsKCR1 is involved in the regulation of very long chain fatty acid synthesis in F. sacchari. These results provide important information for understanding of the pathogenic mechanism of pokkah boeng disease caused by F. sacchari.
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基本信息:
DOI：10.13417/j.gab.042.001283
中图分类号:S435.661
引用信息:
[1]玉权,曾泉,黄振鑫,等.甘蔗镰孢菌β-酮酯酰-辅酶A还原酶基因FsKCR1功能研究[J].基因组学与应用生物学,2023,42(12):1283-1294.DOI:10.13417/j.gab.042.001283.
基金信息:
国家自然科学基金项目(31960031); 广西大学甘蔗专项科研项目(2022GZB010)共同资助
2023-12-02
2023-12-02
2023-12-02