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本研究以人胃癌SGC-7901细胞为实验对象,探讨白花蛇舌草对人胃癌SGC-7901细胞凋亡的影响。使用台盼蓝拒染法检测人胃癌SGC-7901细胞活性,通过普通光学显微镜、荧光显微镜和共聚焦显微镜,观察细胞的形态结构变化,DNA laddy检测DNA片段,Western blotting检测bax和Bcl-2基因的表达,流式细胞术检测细胞周期及线粒体膜电位。结果显示,在一定浓度范围内白花蛇舌草能抑制人胃癌SGC-7901细胞增殖,呈时间和剂量依赖性。24 h、48 h和72 h的半数抑制浓度(IC50)分别为135.886μg/m L,45.84μg/m L和42.56μg/m L。显微镜下观察到细胞皱缩、核裂变、凋亡小体、细胞核裂解、染色质形态改变等现象,DNA出现片段化,细胞阻滞于G1期,Bax蛋白表达上调、Bcl-2的蛋白表达下调。上述表明白花蛇舌草能抑制人胃癌SGC-7901细胞增殖并诱导其凋亡。
Abstract：In this research, the effect of Herba hedyotis diffusae on apoptosis of human gastric cancer SGC-7901 cells was investigated by using the human gastric cancer SGC-7901 cell as the experimental object. The activity of human gastric cancer SGC-7901 cells was detected by using trypan blue staining method. The morphological changes of the cells were observed though ordinary optical microscope, fluorescence microscope and confocal microscopy. DNA laddy detected DNA fragment. Western blot detected the expression of Bax and bcl-2 gene.Flow cytometry detected cell cycle and mitochondrial membrane potential. The results showed that in a certain concentration range of Hedyotis diffusa could inhibit human gastric proliferation. The proliferation of cancer SGC-7901 cells presented a time and dose dependent. The half inhibitory concentration(IC50) of 24 h, 48 h and72 h were 135.886 g/m L, 45.84 g/m L, 42.56 g/m L, respectively. Cell shrinkage, nuclear fission, apoptotic bodies,nuclear fragmentation, chromatin morphology change and other phenomena were observed by microscope. DNA presented fragmentation and cell cycle were arrested in G1 phase. The expression of Bax was up-regulated, while the expression of Bcl-2 protein decreased. The above showed that Hedyotis diffusa could inhibit the proliferation of human gastric cancer SGC-7901 cells and induce apoptosis.
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基本信息:
DOI：10.13417/j.gab.037.003060
中图分类号:R285.5
引用信息:
[1]焦凯贺,邵淑丽,陈丽,等.白花蛇舌草诱导胃癌SGC-7901细胞凋亡[J].基因组学与应用生物学,2018,37(07):3060-3065.DOI:10.13417/j.gab.037.003060.
基金信息:
黑龙江省自然科学基金(No.C201241;C200624);; 黑龙江省教育厅科学技术项目(No.12511611;No.11511447);黑龙江省教育厅基本业务专项(No.135109104)共同资助
2018-07-25
2018-07-25