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考察君山银针茶叶的降血脂及护肝作用。用高脂饲料饲喂小鼠建立高脂血症动物模型,以不同剂量(225 mg/kg,450 mg/kg,900 mg/kg)君山银针茶叶水提物给小鼠每天灌胃,连续4周后检测血清内下列指标的水平:高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)及总胆固醇(TC)。同时观察其对肝组织谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、丙二醛(MDA)、体重、肝体比及肝组织病理变化的影响。实验结果显示,与模型组相比,君山银针茶叶可呈剂量依赖性地降低血清中TC、TG、LDL-C的水平,提高HDL-C的含量。君山银针茶叶还可以显著降低小鼠体重、肝体比及肝组织中MDA含量,极显著提高肝组织中GSH-Px和SOD活性,并减轻了肝脏的脂肪变性程度。HE染色显示肝细胞脂肪颗粒被君山银针茶显著降低,证明了君山银针茶叶有较明显的降血脂和改善肝组织脂肪性损伤的作用。
Abstract：The aim of this study is to investigate the effect of reducing blood lipid and protecting liver of Junshanyinzhen tea on rats. The model of mice with hyperlipidemia was established by feeding with high-fat diet,and Junshanyinzhen tea(225 mg/kg, 450 mg/kg, 900 mg/kg) were intragastrically administered daily to those mice for 4 consecutive weeks. The value of Junshanyinzhen for high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C), triglyceride(TG) and total cholesterol(TC) in blood were evaluated and monitored. Meanwhile, the effect on the glutathione peroxidase(GSH-Px), superoxide dismutase(SOD),malondialdehyde(MDA) of liver tissue, the change of body weight, liver-to-body weight ratio and the pathological change of hepatic tissue were examined. The results showed that, compared with model group, Junshanyinzhen tea decreased the serum TC, TG and LDL-C levels, and increased the level of HDL-C in a dose-dependent manner. In addition, Junshanyinzhen tea substantially reduced the body weight, liver-to-body weight ratio and the level of MDA in hepatic tissue, and increased the GSH-Px and SOD activity. HE staining showed that the fat particles in liver cytoplasm were remarkably reduced by Junshanyinzhen tea. We conclude that Junshanyinzhen tea had good hypolipidemic effect and improved liver tissue damage.
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基本信息:
DOI：10.13417/j.gab.036.003346
中图分类号:R285.5
引用信息:
[1]马蕊,林勇,阳琼芳,等.君山银针茶对高脂血症小鼠的降血脂和改善肝组织损伤作用[J].基因组学与应用生物学,2017,36(08):3346-3351.DOI:10.13417/j.gab.036.003346.
基金信息:
2016年度广西壮族自治区中青年教师基础能力提升项目:茶树花保健作用及其综合开发研究(桂教科研[2016]3号KY2016YB615)资助
2017-08-25
2017-08-25