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旨在利用浙江地区生长正常和生长滞缓两种生长状态下的罗氏沼虾,通过转录组测序技术,比对基因表达量的差异,探究生长滞缓的原因。本研究采用Illumina HiSeqTM4000测序平台,对存在生长差异的罗氏沼虾的鳃、肝胰腺、肌肉三种组织共18个样本进行了转录组测序分析,将得到的基因片段组装后与NR、GO、KEGG、eggNOG、Swiss-Prot共五大数据库比对注释,然后进行表达差异分析。其中从肝胰腺组织共得到至少35 942 944条Clean read,鳃组织共得到至少34 309 548条Clean read,肌肉组织得到至少34 695 288条Clean read,拼接得到243 414条。通过对三种组织生长正常和生长滞缓样本文库分别进行两两对比,本研究在肝胰腺中发现2 323个基因表达呈显著差异(P<0.05),肌肉中1 505个基因表达呈显著差异(P<0.05),鳃组织中1 896个基因表达呈显著差异(P<0.05)。三种组织中得到存在共同差异的基因27个,其中24个基因在三种组织中表达趋势一致,其中14个基因被成功注释,新发现10个未被注释的基因,可能与罗氏沼虾生长密切相关的基因。本研究为进一步探究罗氏沼虾生长缓慢原因提供了基础信息。
Abstract:The aim of this study was to compare the difference of gene expression by transcriptome sequencing technology to explore the reasons of slow growth in the two growth states of Macrobrachium rosenbergii. In this study,using the Illumina HiSeqTM4000 sequencing platform, a transcriptome sequencing analysis was performed on 18 samples of gill, hepatopancreas, and muscle tissues of Macrobrachium rosenbergii with growth differences. The resulting gene fragments were assembled and compared with NR, GO, KEGG, eggNOG and Swiss-Prot for a total of 5 major database annotations followed by expression difference analysis. Of these, at least 35 942 944 were obtained from hepatic and pancreatic tissues, at least 34 309 548 from gill tissues, at least 34 695 288 from muscle tissues, and 243 414 Unigenes by splicing. The expression of 2 323 genes was significantly different in hepatopancreas(P<0.05),the expression of 1 505 genes in muscle was significantly different(P<0.05), and the expression of 1 896 genes in gill tissues was significantly different(P<0.05). There were 27 genes with common differences in the three tissues, 24 of which showed consistent expression trends in the three tissues, 14 of which were successfully annotated, and 10 new unannotated genes, possibly closely related to the growth of Macrobrachium rosenbergii. This study provided basic information for further exploring the reasons for the slow growth of Macrobrachium rosenbergii.
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基本信息:
DOI:10.13417/j.gab.040.000089
中图分类号:S917.4
引用信息:
[1]李雅慧,刘志伟,戴习林.生长滞缓与正常罗氏沼虾转录组差异分析[J].基因组学与应用生物学,2021,40(01):89-100.DOI:10.13417/j.gab.040.000089.
基金信息:
上海市虾类产业技术体系建设项目(沪农科产字(2014)第5号)资助
2021-01-25
2021-01-25