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胞浆内单精子注射(intracytoplasmic sperm injection,ICSI)是一种研究种间受精机制的有效工具。本文旨在采用ICSI方法来构建水牛-猪种间显微授精卵,探讨了水牛-猪种间显微授精卵早期发育并检验了几种提高其发育潜力的方法。研究表明:(1)经ICSI方法制备的水牛-猪种间显微授精卵可以形成双原核,并能够发育到囊胚期;(2)PZM-3相对于SOF而言要更适于水牛-猪种间ICSI胚胎的培养;(3)用甲基化酶抑制剂5-azacytidine(5-Aza C)对水牛精子进行处理可以在一定程度上提高水牛-猪种间ICSI卵的原核形成率,但并不能提高其发育潜力。综上,本研究可为进一步探索水牛-猪种间受精机制及精卵互作奠定基础。
Abstract：Intracytoplasmic sperm injection(ICSI) is an effective tool for investigating the mechanism of interspecies fertilization. The present study was conducted to generate inter-species fertilized oocytes by using of buffalo sperms and porcine oocytes via ICSI. In this study,we studied the early development of buffalo-pig inter-species ICSI fertilized oocytes(termed as buffalo-pig ICSI oocytes),and also examined some methods used for improving the development of such oocytes. We found that(1) the 2 pronucleus formation was indeed occured in these buffalo-pig ICSI oocytes generated via ICSI,and such oocytes could develop to the blastocyst stage;(2) the PZM-3 was examined be more suitable than SOF for culturing buffalo-pig ICSI oocytes;(3) we comfirmed that the DNA methyltransferases(DNMTS),5-azacytidine(5-Aza C),was able to improve,at a certain extent,the 2 pronucleus formation in buffalo-pig ICSI oocytes,whereas with no significant effect on the in vitro development.Summarily,this study lay the foundation for further investigating the fertilized mechanisms of buffalo-pig inter-species fertilization and sperm-oocyte interaction.
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基本信息:
DOI：10.13417/j.gab.035.000579
中图分类号:S828;S823.83
引用信息:
[1]全守能,朱向星,聂骏宇,等.利用胞浆内单精子注射技术研究水牛与猪的种间受精:阶段性报告[J].基因组学与应用生物学,2016,35(03):579-586.DOI:10.13417/j.gab.035.000579.
基金信息:
国家自然科学基金资助项目(31260553);; 广西硕士研究生创新课题(YCSZ2013003)共同资助
2016-03-25
2016-03-25