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为了选育高产二十碳五烯酸(eicosapentaenoic acid, EPA)和二十二碳六烯酸(docosahexaenoic acid,DHA)的裂殖壶菌，本研究采用了紫外诱变的方法对原始菌株B4D1进行诱变，将诱变的突变菌株进行培养和油脂提取，然后用气相色谱仪(GC)进行脂肪酸各组分含量的分析，最终获得一株高产EPA和DHA的菌株11-9E，它的EPA和DHA含量分别达到总油脂的0.47%和41.24%，而原始菌株的EPA和DHA仅占总油脂含量的0.32%和36.92%。相对于原始菌株，突变菌株11-9E的EPA和DHA含量分别提高了46.88%和11.70%。本研究为裂殖壶菌的发酵产业提供了优良菌株，对EPA和DHA的产业化生产具有重大意义。
Abstract：In order to breed the high yield Schizochytrium limacinum about EPA(eicosapent aenoic acid) and DHA(Docosahexaenoic acid), we adopted the method of ultraviolet mutagenesis to treat the original strains B4D1.We cultivated the mutant strains to extract the oil, and then analyzed the fatty acid content of each component by gas chromatograph(GC). At last, we obtained a high yield EPA and DHA strain 11-9E whose EPA and DHA content reached 0.47% and 41.24%, respectively. However, the EPA and DHA of original strain accounted for only0.32% and 36.92% of the total fat content. Compared with the original strains, the EPA and DHA content of mutant strain 11-9E increased by 46.88% and 11.70% respectively. This experiment provides the excllent strains for Schizochytrium fermentation industry and it is of great significance to the industrialization production of EPA and DHA.
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基本信息:
DOI：10.13417/j.gab.040.003055
中图分类号:Q933
引用信息:
[1]张梦娣,赫荣琳,张东远,等.紫外诱变筛选高产EPA和DHA的裂殖壶菌[J].基因组学与应用生物学,2021,40(Z3):3055-3059.DOI:10.13417/j.gab.040.003055.
基金信息:
政府间国际科技创新合作重点专项(2018YFE0107100); 2018年天津市一二三产业融合发展科技示范工程项目(18ZXYENC00150); 2019年度吉林省与中国科学院科技合作高新技术产业化资金项目(2019SYHZ0012)共同资助
2021-10-25
2021-10-25