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组蛋白去乙酰化酶(histone deacetylases,HDACs)可以将组蛋白尾部发生乙酰化的位点去除,进而改变染色体的表观遗传状态。其中,HDAC11是组蛋白去乙酰化酶家族Ⅳ类中唯一成员。为了探究马氏珠母贝组蛋白去乙酰化酶PmHDAC11基因的序列及表达特征,本研究利用RACE技术克隆得到PmHDAC11基因全长并进行结构和表达分析。结果显示,PmHDAC11的cDNA序列全长为2 143 bp,其中5′-UTR为124 bp,3′-UTR为516 bp,包含29 bp polyA尾巴。开放阅读框(ORF)长度为1 503 bp,编码500个氨基酸。根据推导的氨基酸序列预测其相对分子量为56 371.52 Da,理论等电点为7.66,不稳定系数为38.97,总平均亲水性为-0.284。Signal 4.1和TMHMM Server 2.0显示,PmHDAC11无信号肽与跨膜结构域。系统进化树显示,PmHDAC11与美洲牡蛎(Crassostrea virginica)聚为一簇,与传统分类结果基本一致。多序列比对显示,PmHDAC11与其他物种的HDA C11有较高的保守性,与牡蛎(Crassostrea gigas)的同源序列达66.31%。LPS刺激后,PmHDAC11基因在马氏珠母贝血液中的相对表达量在6 h上调,后在12 h恢复到正常水平,在24 h后降低,说明其可能与马氏珠母贝机体的免疫反应有关。本研究可为进一步探讨PmHDAC11基因在马氏珠母贝中的生物学特性及功能提供依据。
Abstract：Histone deacetylases(HDACs) could remove the acetylation sites on the histones and change the epigenetic state of chromosomes.Among them,HDAC11 is the only member of the histone deacetylase family Ⅳ class.In order to explore the characteristics of histone deacetylases 11 gene in Pinctada fucata martensii(PmHDA C11),using RACE technology,we have got the cDNA full length of HDAC11.The results showed that the full-length of cDNA sequence of PmHDAC11 was 2 143 bp,of which the 5'-UTR was 124 bp,the 3'-UTR was 516 bp,containing a 29 bp polyA tail.The open reading frame(ORF) is 1 503 bp in length and encodes 500 amino acids.According to the deduced amino acid sequence,the relative molecular weight was predicted to be 56 371.52 Da,the theoretical isoelectric point was 7.66,the instability coefficient was 38.97,and the total average hydrophilicity was -0.284.The results of Signal 4.1 Server and TMHMM Server 2.0 indicated that PmHDAC11 had no signal peptide and transmembrane domain.The phylogenetic tree showed that HDAC11 were clustered together with that from Crassostrea virginica,which was consistent with the traditional classification results.Multiple sequence alignment showed that PmHDAC11 was highly conserved with other species of HDAC11,and the homologous identity with oyster(Crassostrea gigas) reached to 66.31%.After LPS stimulation,the relative expression of PmHDAC11 gene in the blood of P.f.martensii at 6 h and then decreased to normal level at 12 h,indicating that it may be related to the immune response of P.f.martensii.This study provides basis for the further study of the biological characteristics of PmHDA C11 gene in P.f.martensii.
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基本信息:
DOI：10.13417/j.gab.038.004885
中图分类号:S917.4
引用信息:
[1]杨帅,潘晓燕,曹艳飞,等.马氏珠母贝组蛋白去乙酰化酶HDAC11基因的克隆与表达分析[J].基因组学与应用生物学,2019,38(11):4885-4893.DOI:10.13417/j.gab.038.004885.
基金信息:
国家自然科学基金(31672626);; 广东省海洋与渔业局项目(Z2015004);; 广东省高等学校优秀青年教师培养计划(YQ2015090);; 广东海洋大学创新强校(GDOU2016050249)共同资助
2018-09-25
2018-09-25
2018-09-25