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水稻(Oryza sativa L.)是世界上最重要的作物之一，而分蘖角度是水稻的重要性状，它影响着水稻的光合作用面积。LAZY1是水稻中第1个被鉴定的调控水稻分蘖角度的基因，然而LAZY1作为转录因子，它结合的下游基因尚不清楚。本研究通过染色质免疫共沉淀测序(ChIP-Seq)技术，研究LAZY1在全基因组水平的结合位点，分析其可能调控的基因。根据ChIP-Seq结果，我们发现大量的peaks除了坐落在基因间隔区外，还坐落在基因的启动子上，暗示LAZY1可能调控大量下游基因的表达。通过GO功能分析和KEGG富集分析，发现LAZY1可能调控一类影响分子代谢和修饰的基因。经过筛选，我们获得了51个LAZY1下游候选基因，根据其中一些已被报道的基因，分析了LAZY1的下游调控网络，为今后的水稻分蘖角度研究提供参考。
Abstract：Rice is one of the most important crops in the world, and tiller angle is an important trait of rice, which affects the photosynthesis area. LAZY1 is the first identified gene that regulated rice tiller angle. As a transcription factor, its downstream binding genes remain unknown. In this research, we identified the binding sites of LAZY1 in genome-wide level through Chromatinimmunoprecipitation-based sequencing(ChIP-Seq), and analyzed the genes it might regulate. We found a large number of peaks located not only in intergenic regions, but also in promoters, indicating that a large number of downstream genes were regulated by LAZY1. We also found that LAZY1 may regulate some genes involved in macromolecular metabolism and modification by GO classification and KEGG pathway enrichment analysis. After screening, we obtained 51 downstream candidate genes of LAZY1. Next, we analyzed the downstream regulating network of LAZY1 based on some reported genes. This research provided a reference for future studies on rice tiller angle.
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基本信息:
DOI：10.13417/j.gab.041.001539
中图分类号:S511
引用信息:
[1]朱亮,薛蓬勃,李国强,等.全基因组结合位点分析揭示LAZY1控制水稻分蘖角度的下游调控网络[J].基因组学与应用生物学,2022,41(07):1539-1549.DOI:10.13417/j.gab.041.001539.
基金信息:
国家自然科学基金项目(32060174、32160079); 广西自然科学基金项目(2020GXNSFAA297211)共同资助
2022-06-07
2022-06-07
2022-06-07