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本研究利用同源克隆的方法从东农50中克隆得到一个钾离子通道相关基因,命名为GmA KT1。生物信息学分析Gm AKT1含有一个长2 628 bp的开放阅读框,编码875个氨基酸,推测为亲水跨膜蛋白。组织特异性表达分析发现GmA KT1在大豆根部表达量最高。根据TA连接特征将克隆片段分别正向、反向插入PCXSN-1250载体,得到植物过表达载体PCXSN-GmA KT1(+)及反义表达载体PCXSN-GmA KT1(-),为进一步研究大豆GmA KT1的转化、分析功能,及改良大豆品种提供科学依据。
Abstract:K+ Channel Gene GmA KT1 was isolated from soybean DN50 by homology cloning.GmA KT1 contains a 2 628 bp open reading frame,encoding a 875 amino acids protein.The GmA KT1 protein was found to be more hydrophilic,and transmembrane protein.The mR NA abundance of GmA KT1 in roots was higher than that of other tissues.According to TA connection characteristic,we constructed the expression vector of PCXSN-GmA KT1(+)and PCXSN-GmA KT1(-),this might lay foundation for further studying the functional of GmA KT1.
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基本信息:
DOI:10.13417/j.gab.034.000143
中图分类号:S565.1
引用信息:
[1]王英琪,李永光,王涛,等.大豆GmAKT1基因的克隆及其正反义植物表达载体构建[J].基因组学与应用生物学,2015,34(01):143-148.DOI:10.13417/j.gab.034.000143.
基金信息:
抗逆转基因大豆新品种培育(2014ZX08004002)资助
2015-01-25
2015-01-25